Adam17 (NM_009615) Mouse Untagged Clone

SKU MC221841

Adam17 (untagged) - Mouse a disintegrin and metallopeptidase domain 17 (Adam17), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Adam17
• Synonyms: CD156b; Tac; Tace
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221841 representing NM_009615; Red=Cloning site Blue=ORF; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGCGGCGTCTCCTCATCCTGACCACTTTGGTGCCTTTCGTCCTGGCACCCCGACCTCCGGAGGAAG; CAGGCTCTGGCTCCCATCCGCGACTTGAGAAGCTTGATTCTTTGCTCTCAGACTACGACATCCTCTCCTT; AGCTAATATTCAGCAGCACTCCATAAGGAAAAGGGATCTACAGTCTGCGACACACTTAGAAACATTACTA; ACTTTTTCAGCTTTGAAAAGACATTTTAAATTATACTTGACATCAAGTACCGAACGCTTTTCACAAAACT; TGAGAGTCGTGGTGGTGGACGGGAAAGAAGAAAGCGAGTACAGCGTGAAGTGGCAGAACTTCTTCAGTGG; TCACGTGGTTGGTGAGCCTGACTCTAGGGTTCTAGCCCACATAGGAGATGATGATGTTACAGTGAGAATC; AACACAGATGGGGCAGAATATAACGTAGAGCCACTTTGGAGGTTTGTCAATGATACTAAAGATAAACGAA; TGCTGGTGTATAAGTCTGAAGATATCAAGGATTTTTCACGTTTGCAGTCTCCAAAAGTATGTGGTTATTT; AAATGCAGATAGTGAAGAGCTGCTTCCAAAAGGGCTCATAGACAGAGAGCCATCTGAAGAGTTTGTTCGT; CGAGTGAAGAGACGAGCTGAACCTAACCCCTTGAAGAATACTTGTAAATTACTGGTGGTAGCAGATCATC; GATTTTATAAATACATGGGCCGTGGAGAAGAGAGCACCACTACAAATTACTTAATAGAGCTAATTGACCG; AGTTGATGACATATACCGGAACACGTCGTGGGATAATGCAGGGTTTAAAGGGTATGGAGTGCAGATAGAG; CAGATTCGAATTCTCAAGTCTCCACAAGAGGTAAAACCTGGTGAAAGACACTTCAATATGGCAAAAAGTT; TCCCAAACGAAGAGAAGGATGCTTGGGATGTGAAGATGCTATTAGAGCAATTTAGCTTTGATATAGCTGA; AGAAGCATCTAAAGTCTGCCTGGCTCATCTTTTCACGTACCAGGATTTTGATATGGGAACTCTTGGATTA; GCTTACGTTGGTTCTCCCAGAGCAAACAGTCATGGAGGGGTTTGTCCAAAAGCTTATTACAACCCAACTG; TGAAGAAAAACATCTATTTAAATAGTGGTCTGACTAGTACTAAAAATTATGGCAAAACTATTCTCACAAA; GGAAGCTGACCTGGTTACAACTCATGAATTGGGACATAATTTTGGAGCAGAACATGACCCTGATGGGCTA; GCAGAATGTGCCCCAAATGAGGACCAAGGAGGAAAGTATGTCATGTATCCCATAGCTGTGAGCGGTGACC; ACGAGAATAATAAGATGTTTTCAAACTGCAGTAAACAGTCCATCTACAAGACCATAGAAAGTAAGGCTCA; AGAGTGCTTCCAGGAGCGCAGCAACAAGGTGTGTGGCAACTCCAGGGTGGATGAAGGAGAGGAGTGTGAC; CCGGGTATTATGTACCTGAACAACGACACCTGCTGCAATAGTGACTGCACACTGAAGCCGGGTGTGCAGT; GCAGTGATAGGAACAGTCCTTGCTGTAAAAACTGTCAGTTTGAGACGGCGCAGAAGAAGTGCCAGGAGGC; TATTAATGCTACATGCAAAGGAGTGTCTTACTGCACAGGGAATAGCAGTGAGTGCCCCCCACCCGGAGAT; GCTGAAGATGACACTGTGTGCTTGGACCTTGGCAAGTGCAAGGCTGGGAAATGCATCCCTTTCTGCAAGA; GGGAGCAGGAGCTGGAGTCCTGCGCATGCGTTGACACTGACAACTCGTGCAAGGTGTGCTGCAGGAACCT; TTCTGGCCCGTGTGTGCCGTACGTCGATGCAGAGCAAAAGAACTTGTTTTTGAGGAAAGGGAAGCCATGT; ACAGTAGGGTTTTGCGACATGAATGGCAAATGTGAGAAACGAGTACAGGACGTAATTGAGCGATTTTGGG; ATTTCATTGACCAGCTGAGCATCAACACTTTTGGGAAGTTTCTGGCAGATAACATCGTTGGGTCTGTTCT; GGTTTTCTCCTTGATATTTTGGATTCCTTTCAGCATTCTTGTCCACTGTGTGGATAAGAAACTGGACAAG; CAGTATGAATCCCTGTCTCTGTTTCATCACAGTAACATTGAGATGCTGAGCAGCATGGACTCAGCATCTG; TTCGCATCATCAAGCCCTTTCCTGCACCCCAGACTCCAGGTCGTCTGCAGGCCCTGCAGCCAGCTGCCAT; GATGCCGCCAGTACCTGCAGCTCCAAAACTGGACCACCAGAGGATGGATACCATCCAGGAAGACCCCAGC; ACAGACTCACATGCAGATGATGACGGTTTTGAGAAGGACCCCTTCCCCAACAGCAGCACAGCTGCCAAGT; CCTTTGAGGATCTCACAGACCACCCAGTCACCAGGAGCGAAAAGGCGGCCTCATTCAAGCTGCAGCGTCA; GAGCCGAGTTGACAGCAAAGAGACAGAGTGCTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009615
• Insert Size: 2484 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC094655, AAH94655
• RefSeq Size: 4118 bp
• RefSeq ORF: 2484 bp
• Locus ID: 11491
• UniProt ID: Q9Z0F8
• Cytogenetics: 12 8.3 cM
• Summary: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the encoded protein die in utero or fail to survive beyond one week of age. Alternative splicing results in multiple transcript variants encoding different isoforms, some of which may undergo similar processing. [provided by RefSeq, May 2016]; Transcript Variant: This variant (1) encodes the predominant isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.