Tlr1 (NM_030682) Mouse Untagged Clone

SKU MC221701

Tlr1 (untagged) - Mouse toll-like receptor 1 (Tlr1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Tlr1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221701 representing NM_030682; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACTAAACCAAATTCCCTCATCTTCTACTGTATCATTGTTTTAGGACTGACACTTATGAAAATCCAAT; TATCTGAGGAATGTGAGCTTATCATAAAGAGGCCAAACGCAAACCTTACCAGAGTGCCCAAGGACCTACC; CTTGCAAACAACTACTTTAGATCTATCACAAAACAATATATCTGAGCTTCAGACTTCTGACATCCTCTCA; TTGTCCAAGCTGAGGGTCCTGATAATGTCCTACAACAGACTCCAGTATCTTAATATCAGTGTTTTCAAAT; TCAACACAGAGCTGGAATATTTGGATTTGTCCCACAATGAGCTAAAGGTGATCTTGTGCCACCCAACAGT; CAGCCTCAAGCATTTGGACCTCTCCTTTAATGCCTTTGATGCCCTGCCTATATGCAAAGAATTTGGCAAC; ATGTCCCAACTACAGTTCCTGGGGTTGAGCGGTTCTCGGGTACAAAGTTCAAGTGTGCAGCTGATTGCTC; ATTTGAACATCAGTAAGGTTTTGCTGGTGTTAGGAGATGCTTATGGGGAAAAAGAAGACCCCGAATCTCT; TCGGCACGTTAGCACTGAGACTCTGCATATTGTTTTCCCGTCGAAAAGAGAATTCCGTTTTCTTCTGGAT; GTGTCCGTCAGCACTACGATCGGTTTGGAACTGTCTAACATCAAGTGTGTGCTTGAAGACCAGGGCTGCT; CTTATTTCTTACGTGCTTTGTCAAAGCTTGGAAAGAATCTGAAGCTCTCAAATCTTACCCTGAACAATGT; GGAAACAACGTGGAATTCCTTCATTAATATCCTCCAGATAGTTTGGCATACGCCAGTCAAATATTTCTCA; ATTTCAAATGTGAAGCTACAAGGTCAACTTGCCTTCAGGATGTTCAATTATTCTGACACTTCTCTGAAGG; CTTTGTCGATACATCAAGTTGTCACTGATGTCTTCAGCTTCCCCCAAAGTTACATATACAGTATCTTTGC; CAATATGAACATCCAAAACTTTACAATGTCTGGAACACACATGGTCCACATGCTGTGCCCGTCCCAAGTT; AGCCCATTTCTGCATGTGGACTTTACAGATAACCTTTTAACAGACATGGTTTTTAAAGACTGTAGAAACT; TAGTTAGATTGAAAACACTTAGTTTACAAAAGAATCAGTTAAAAAACCTTGAGAATATAATCCTCACATC; TGCAAAGATGACATCCCTACAAAAACTAGACATTAGCCAGAATTCTCTAAGGTACAGCGATGGGGGAATC; CCATGCGCCTGGACCCAGAGTTTGTTAGTTTTAAATTTGTCTTCGAATATGCTTACAGGCTCTGTCTTCA; GATGCTTACCTCCCAAAGTCAAGGTCCTTGACCTTCACAACAACAGGATAATGAGCATCCCTAAAGATGT; CACCCACCTGCAGGCTTTGCAGGAACTCAATGTAGCATCCAACTCCTTAACTGACCTTCCTGGGTGCGGG; GCCTTCAGCAGCCTTTCTGTGCTGGTCATCGACCATAACTCAGTTTCCCATCCCTCTGAGGATTTCTTCC; AGAGCTGTCAGAATATTAGATCCCTAACAGCGGGAAACAACCCATTCCAATGCACATGTGAGCTGAGGGA; CTTTGTCAAGAACATAGGCTGGGTAGCAAGAGAAGTGGTGGAGGGCTGGCCTGACTCTTACAGGTGTGAC; TACCCAGAAAGCTCTAGGGGAACTGCACTGAGGGACTTCCACATGTCTCCACTATCCTGTGATACTGTTC; TGCTGACTGTCACCATCGGGGCCACTATGCTGGTGCTGGCTGTCACTGGGGCTTTCCTCTGTCTCTACTT; TGACCTGCCCTGGTATGTGAGGATGCTGTGTCAGTGGACACAGACCAGGCACAGGGCCAGGCACATCCCC; TTAGAGGAACTCCAGAGAAACCTCCAGTTCCATGCTTTTGTCTCATACAGTGGGCATGATTCTGCCTGGG; TGAAGAACGAATTACTACCCAACCTAGAGAAAGATGACATCCAGATTTGCCTCCATGAGAGGAACTTTGT; CCCTGGCAAGAGCATTGTGGAGAACATCATCAATTTCATTGAGAAGAGTTACAAGTCCATCTTTGTGCTG; TCTCCCCACTTCATCCAGAGTGAGTGGTGTCATTATGAACTCTATTTTGCCCATCACAATCTCTTCCATG; AAGGCTCTGATAACTTAATCCTCATCTTGCTGGCACCCATTCCCCAGTACTCCATCCCTACCAATTACCA; CAAGCTCAAAACTCTCATGTCACGAAGGACCTATCTGGAATGGCCCACAGAGAAGAACAAGCATGGACTT; TTTTGGGCAAACCTAAGAGCATCCATTAATGTTAAGCTGGTTAACCAGGCAGAAGGAACGTGTTACACAC; AGCAATAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_030682
• Insert Size: 2388 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030682.2, NP_109607.1
• RefSeq Size: 2721 bp
• RefSeq ORF: 2388 bp
• Locus ID: 21897
• UniProt ID: Q9EPQ1
• Cytogenetics: 5 33.53 cM
• Summary: Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (By similarity). Acts as a coreceptor for M.tuberculosis lipoproteins LprG, LpqH and PhoS1 (pstS1), in conjunction with TLR2 and for some but not all lipoproteins CD14 and/or CD36. The lipoproteins act as agonists to modulate antigen presenting cell functions in response to the pathogen (PubMed:19362712).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.