Gcfc2 (NM_177884) Mouse Untagged Clone

SKU MC221471

Gcfc2 (untagged) - Mouse expressed sequence AW146020 (AW146020), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gcfc2
• Synonyms: A130099G21; AW146020; GCF2; Tcf9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221471 representing NM_177884; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTCTCAGGCCCCAGAGGACTTTCAGGCGGCGCCAGGTCGAGTCCAGCGATAGCGATAGCGATAGCG; ACGGCGCGAAGGAGCAGAGTGCGGAGGAGCCGGCGTCTGCCGGCGGCCGAACAGAGGGAGCGGAGCGGCC; GCGCGGGGCCCGCAGCGCCCGGGGCCGAGGTCGGGTCTGGGCGAGTTCCCGCCGCAGCCCGGGAGCTGCT; CCCCGCGGGGACGGCGGAGCAGAATGCAGAACAGCTGAGCTCTCAACGGATGAAGAGGAAGGAACACATA; CCTTGACAGGAAGTAAGGGTGATCGGAGTCCGTCCTCTGATAGCTCTTGCTCTCTGGAAGAAAGAGACGT; TTCACCCATAGTTGAGATCCCCGATGCAGCTTTTATTCAGGCAGCCCGCAGAAAACGGGAGTTGGCCAGG; ACCCCAGGGGACTACATTTCATTGGATGTAAACCACTCCTGCTCCACTTCTGACTGTAAGAGAAGCAATG; AAGAGGACCCAGAGAGCGATCCTGATGACCACGAAAAGAGAATACTGTTTACCCCAAAGCCTCAAACACT; CCGGCAAAGAATGGCTGAAGAAACGTCAATCAGGAGTGAGGAATCAAGTGAAGAGAGTCAGGAAGATGAA; AACCAAGACATCTGGGAGCAGCAGCAGATGAGGAAAGCAGTCAGGATCCCGGCGGGACAAAATACAGACC; TTTCCCACAGCAGTAAGTCTCAAACCCTGAAGAAGTTTGATACTTCCATTTCATTTCCACCGGTAAATTT; GGAAATTATAAAGAAACAGTTAAATAATAGATTAACATTACTACAGGAGTCTCATCGCTCACACCAGAGA; GAATATGAAAAATATGAACAAGACATCAAGAGTTCAAAGACGGCTATCCAGAATCTTGAGAGTGCATCAG; ACCATGCTCAAAACTACAGATTCTACAGGGGCATGAAAAGTTATGTGGAAAATATAATTGATTGTCTAAA; TGAAAAGATTGTCAGCATTGTGGAACTAGAATCATCCATGTACACGTTGCTTTTAAAACGGTCAGAAGCA; CTTTTGAAACGCAGGCAAGATGAATTAAAATGCGAATCATCATATTTGCAGCAGTTATCACGCAAAGATG; AGACATCGGCAAATGGAAGCTTGGCTGTGGATGAAAAGGATCAACGGATTTTAGAAGAGATTGAAGCTCG; GAGGATGCAGAGAAGACAGGCCAGGGAGCTCTCGGGCAGCTGTGACCATCAGGAAGGGATGTCTAGTGAT; GATGAGCTGTCTCCTGCAGAGATGACTAACTTCCACAAGTGCCAAGGTGACATTTTACAGGATTGTAAGA; AAGTTTTTGAAGATGTGCATGATGATTTTTGTAACGTCCAGAATATTTTATTGAAATTTCAGCAGTGGCG; AGAAAAGTTTCCTGATTCCTACTATGAAGCCTTCGTTGGTTTCTGCTTACCAAAGCTTTTAAGTCCCCTG; ATACGAGTTCAGTTGCTTGACTGGAATCCTCTTAAGATGGACTCCATAGGTCTAGACAAGATGCCCTGGT; TCACAGCTATAACAGAGTTTATGGAGAGCAGCATGGACGATATAGGGAAGGAAGATGGTTCTGATAAAAA; AATCTTGGCTGCTGTCATCAACAAAACAGTTGTTCCTCGACTTACAGATTTTGTAGAGACCATTTGGGAT; CCCCTGTCAACCTCACAGACAAGAAGTTTAACAGTGCACTGCAGAGTGGCTTTTGAACAGTTCGCTTCTG; AAAATGAAGTTAGTAAAAATAAACAGGATTTACTTAAATCTATTGTTGCAAGAATGAAGAAGTCAATAGA; AGATGATATTTTTATTCCTCTATACCCAAAGAGCTCTGAAGAAGGAAAAATGTCACCACACTCCAAGTTC; CAAGAAAGACAGTTCTGGGGAGCTCTAAAGCTCTTCCGAAATATTCTTCTTTGGAATGGCCTTCTCCCAG; ATGACACCTTGCAAGATCTGGGACTGGGGAAGCTGCTGAATCGCTACCTTATCATCTCTCTTACTAATGC; CGTCCCTGGACCAGATGTGGTTAAAAAGTGCAGCCAGATAGCAGCATGTCTACCAGAAAGATGGTTTGAG; AACTCTGCTATGAGGACATCAATTCCCCAGCTAGAAAACTTCATTAAGTTTTTGTTGCAGTCTGCACAGA; AATTATCCTCAAGTGAATTCAGGAATGAAGTCAGTGAAATAATTCTTATCCTGGTGAAGGTAAAAGCTCT; GACTCAAGCCGAATCCCTCAGAGAAGAGCGTCCCCTGGAGCCCCTCCCAGCCCAGAGTACAGGTGTTTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177884
• Insert Size: 2310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_177884.3, NP_808552.2
• RefSeq Size: 3375 bp
• RefSeq ORF: 2310 bp
• Locus ID: 330361
• UniProt ID: Q8BKT3
• Cytogenetics: 6 C3
• Summary: Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons (By similarity).[UniProtKB/Swiss-Prot Function]