Mmel1 (NM_013783) Mouse Untagged Clone

SKU MC221441

Mmel1 (untagged) - Mouse membrane metallo-endopeptidase-like 1 (Mmel1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Mmel1
• Synonyms: Mell1; NEP2; NEPII; Nl1; SEP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC221441 representing NM_013783; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTGGAGAGAGCAGGCTGGTGTCGGAAGAAGTCCCCAGGCTTCGTGGAGTATGGGCTGATGGTGCTGC; TGCTGCTGCTGTTGCTGGGAGCCATAGTGACTCTGGGTGTCTTCTACAGCATAGGGAAGCAGCTGCCCCT; CTTAACTAGCCTGCTACACTTCTCCTGGGATGAGAGGACGGTTGTAAAACGAGCCCTCAGGGATTCATCA; CTGAAAAGTGACATCTGCACCACCCCAAGCTGTGTGATAGCAGCTGCCAGAATCCTCGAAAACATGGACC; AATCGAGGAACCCCTGTGAAAACTTCTACCAGTACGCTTGCGGAGGCTGGCTGAGGCACCACGTGATCCC; AGAGACCAACTCCAGATACAGCGTCTTTGACATCCTTCGGGACGAGCTGGAGGTTATCCTCAAAGGGGTG; CTGGAGGATTCCACTTCCCAGCATCGCCCGGCCGTGGAGAAGGCCAAGACACTATATCGCTCCTGCATGA; ACCAAAGTGTGATCGAGAAGAGAGACTCTGAGCCCCTGCTGAGCGTCTTAAAAATGGTAGGAGGTTGGCC; TGTGGCCATGGATAAGTGGAACGAGACCATGGGCCTCAAGTGGGAACTGGAGCGACAGTTGGCTGTGTTG; AACTCGCAGTTCAACAGGCGGGTCCTCATCGACCTCTTCATCTGGAATGACGACCAGAACTCCAGCCGGC; ATGTCATCTACATAGACCAGCCCACCTTGGGCATGCCATCCCGGGAGTACTATTTCCAGGAGGACAACAA; CCACAAGGTACGGAAAGCCTACCTGGAGTTCATGACGTCAGTGGCCACTATGCTTAGGAAAGACCAGAAC; CTGTCCAAGGAGAGCGCCATGGTGCGGGAGGAGATGGCGGAGGTGCTGGAACTGGAGACGCATCTGGCCA; ACGCCACAGTCCCCCAGGAGAAAAGGCATGATGTCACTGCCCTGTACCACCGAATGGACCTGATGGAGCT; ACAGGAAAGGTTTGGTCTGAAGGGGTTTAACTGGACTCTCTTCATACAAAACGTGTTGTCTTCTGTGGAA; GTCGAGCTGTTCCCAGATGAGGAGGTGGTGGTCTACGGCATCCCCTACCTGGAGAATCTGGAGGATATCA; TTGATAGCTACTCAGCACGGACCATGCAGAACTACCTGGTATGGCGCCTGGTGCTAGATCGAATTGGCAG; CCTGAGCCAGAGATTCAAAGAGGCGCGTGTGGACTACCGCAAGGCGCTGTACGGCACGACCGTGGAGGAG; GTACGCTGGCGAGAGTGTGTCAGCTATGTCAACAGTAACATGGAGAGCGCCGTGGGCTCCCTCTACATCA; AGCGGGCCTTCTCCAAGGACAGCAAGAGCACGGTCAGAGAGCTGATTGAGAAGATAAGGTCCGTGTTTGT; GGATAACCTGGATGAGCTGAACTGGATGGACGAGGAATCCAAGAAGAAGGCCCAGGAAAAGGCCATGAAT; ATACGGGAACAGATTGGCTACCCTGACTACATTTTGGAAGATAACAATAAACACCTGGATGAGGAATACT; CCAGTTTGACTTTCTATGAGGACCTGTATTTTGAGAACGGACTTCAGAACCTCAAGAACAATGCCCAGAG; GAGCCTCAAGAAGCTTCGGGAAAAGGTGGACCAGAATCTCTGGATCATCGGGGCTGCAGTGGTCAATGCA; TTCTACTCCCCAAACAGAAACCAGATCGTCTTTCCAGCAGGGATTCTCCAGCCGCCCTTCTTCAGCAAGG; ACCAACCACAGTCCTTGAATTTTGGGGGCATCGGGATGGTGATTGGGCACGAGATCACACACGGCTTTGA; TGATAATGGTCGTAACTTTGACAAGAACGGCAACATGCTGGACTGGTGGAGTAACTTCTCGGCCCGGCAC; TTCCAACAGCAGTCGCAATGCATGATCTATCAGTACGGCAACTTCTCTTGGGAACTAGCAGACAACCAGA; ATGTGAACGGATTCAGTACCCTCGGGGAGAACATTGCCGACAACGGAGGTGTGCGACAGGCATACAAGGC; TTACCTACGGTGGCTGGCTGATGGCGGCAAAGATCAGCGACTGCCGGGACTGAACCTGACCTATGCCCAG; CTTTTCTTCATCAACTATGCCCAGGTGTGGTGTGGGTCCTATAGGCCGGAGTTCGCCGTCCAGTCCATCA; AGACGGACGTCCACAGTCCTCTTAAGTACAGGGTGCTGGGCTCACTACAGAACCTGCCAGGCTTCTCTGA; GGCATTCCACTGCCCACGAGGCAGCCCCATGCACCCCATGAAGCGATGTCGCATCTGGTAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013783
• Insert Size: 2301 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013783.2, NP_038811.2
• RefSeq Size: 2948 bp
• RefSeq ORF: 2301 bp
• Locus ID: 27390
• UniProt ID: Q9JLI3
• Cytogenetics: 4 E2
• Summary: Metalloprotease involved in sperm function, possibly by modulating the processes of fertilization and early embryonic development. Degrades a broad variety of small peptides with a preference for peptides shorter than 3 kDa containing neutral bulky aliphatic or aromatic amino acid residues. Shares the same substrate specificity with MME and cleaves peptides at the same amide bond.[UniProtKB/Swiss-Prot Function]