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Bard1 (NM_007525) Mouse Untagged Clone

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SKU
MC221420
Bard1 (untagged) - Mouse BRCA1 associated RING domain 1 (Bard1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $701.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Bard1
Synonyms ENSMUSG00000060893; ENSMUSG00000073653
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221420 representing NM_007525
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACGCCGGCCGCCGAGGGTCTGCTCTGGGAACCAGCCTGCTCCCGTGCCCGCCATGGAGCCGGCTA
CCGACGGGCTTTGGGCCCACAGCCGCGCGGCGCTTGCCCGCCTGGAGAAGCTGCTGCGCTGCTCCCGCTG
TGCTAATATTCTGAAGGAGCCCGTGTGCTTAGGAGGATGTGAGCACATCTTCTGTAGTGGTTGTATAAGT
GACTGTGTTGGCTCAGGATGCCCAGTGTGTTACACCCCAGCCTGGATCCTAGACCTCAAGATAAACCGAC
AATTGGACAGCATGATCCAGCTTTCTAGTAAGCTCCAAAATTTGCTCCATGACAATAAAGATTCAAAAGA
CAACACATCTAGGGCAAGTTTATTTGGTGATGCAGAAAGGAAGAAGAATTCAATAAAAATGTGGTTTAGT
CCTCGAAGTAAGAAGGTTAGATATGTTGTGACTAAAGTGTCAGTACAAACACAGCCTCAAAAGGCAAAGG
ACGACAAAGCCCAGGAAGCCTCAATGTATGAATTTGTTTCCGCAACTCCCCCTGTAGCTGTTCCTAAGAG
TGCTAAAACAGCTTCTAGAACATCTGCAAAAAAGCATCCAAAGAAATCTGTAGCTAAGATCAACAGGGAG
GAGAATTTAAGGCCAGAAACAAAGGATAGTAGATTTGATTCCAAAGAGGAGCTGAAGGAAGAGAAGGTTG
TCTCCTGTAGCCAAATACCAGTTATGGAGAGACCACGGGTAAATGGTGAAATAGACTTATTAGCAAGTGG
TTCTGTTGTAGAACCTGAATGTTCTGGAAGCTTGACTGAAGTCTCTTTACCATTGGCTGAGCATATAGTG
TCTCCAGACACTGTGAGCAAGAATGAAGAGACTCCGGAGAAGAAGGTCTGTGTAAAAGATCTTCGTTCAG
GAGGGAGTAATGGAAATCGTAAAGGCTGCCACAGGCCTACCACTTCTACTTCTGACAGTTGTGGGAGCAA
CATTCCGAGCACCAGCAGAGGCATCGGTGAGCCAGCATTGCTTGCAGAAAACGTAGTGTTGGTTGACTGC
TCTTCACTGCCTTCAGGCCAGCTACAGGTTGATGTCACACTCAGGAGAAAGAGTAACGCATCAGATGACC
CCCTTAGCCTTTCACCGGGCACACCCCCACCTCTGCTGAACAATTCAACTCACAGACAAATGATGTCAAG
CCCCTCCACAGTGAAGCTGTCTTCTGGTATGCCAGCCAGGAAAAGAAATCACAGAGGAGAGACCTTACTT
CATATTGCTTCTATTAAGGGTGATATACCTTCTGTTGAATACCTCTTGCAAAATGGAAACGACCCAAATG
TTAAAGACCATGCTGGATGGACACCGTTGCATGAAGCCTGCAGTCATGGGCACCTGAAGGTAGTGGAGTT
GCTGCTCCAGCATAATGCCCTGGTGAACACCCCTGGCTATCAGAATGACTCGCCACTCCACGATGCCGTC
AAGAGTGGCCACATCGATATAGTCAAGGTGTTACTGTCCCACGGTGCTTCCAGGAATGCTGTTAACATAT
TTGGTGTGCGGCCTGTGGACTATACAGACAATGAGAATATAAGATCATTATTGCTGCTGCCAGAGGAGAA
TGAATCATTCTCAACTAGCCAGTGCTCTATCGTGAACACCGGGCAGCGAAAGAATGGGCCGCTGGTATTT
ATAGGCAGTGGGCTTTCTTCACAGCAGCAGAAAATGCTCAGCAAACTTGAGACAGTTCTTAAGGCTAAAA
AGTGTATGGAATTTGACAGTACAGTAACTCATGTCATTGTTCCTGATGAGGAAGCGCAGAGTACTCTGAA
GTGTATGCTTGGGATTCTCAGTGGATGCTGGATCCTGAAGTTTGATTGGGTGAAAGCCTGTCTGGACAGC
AAAGTACGTGAGCAGGAAGAAAAGTATGAAGTTCCTGGAGGTCCACAGAGGAGCAGGCTCAACAGAGAGC
AGCTGTTGCCAAAGCTGTTTGATGGATGCTACTTCTTTTTGGGGGGAAACTTCAAGCATCATCCAAGGGA
TGACCTCCTTAAGCTCATTGCTGCAGCAGGAGGCAAAGTCCTGAGTCGCAAGCCCAAGCCAGACAGTGAT
GTGACTCAGACCATCAACACGGTTGCATACCATGCCAAGCCTGAGTCGGATCAGCGCTTCTGTACGCAGT
ACATCGTCTACGAAGACCTGTTTAACTGTCACCCAGAGAGGGTTCGGCAGGGCAAAGTCTGGATGGCTCC
GTCCACCTGGCTTATCAGCTGTATAATGGCCTTTGAATTGCTTCCTCTTGACAGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_007525
Insert Size 2298 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC128370, AAI28371
RefSeq Size 2386 bp
RefSeq ORF 2298 bp
Locus ID 12021
UniProt ID O70445
Cytogenetics 1 C3
Summary E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007525" in other vectors (4)
SKU Description Size Price
MG217719 Bard1 (tGFP-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1), (10ug) 10 ug
$901.00
MR217719 Bard1 (Myc-DDK-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) 10 ug
$589.00 $701.00
MR217719L3 Lenti ORF clone of Bard1 (Myc-DDK-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) 10 ug
$1,001.00
MR217719L4 Lenti ORF clone of Bard1 (mGFP-tagged) - Mouse BRCA1 associated RING domain 1 (Bard1) 10 ug
$1,001.00

file-alt Citations

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