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Krit1 (NM_030675) Mouse Untagged Clone

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SKU
MC221186
Krit1 (untagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $742.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Krit1
Synonyms 2010007K12Rik; A630036P20Rik; AA432855; AI450393; AI643869; BB155247; BB235701; Ccm1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC221186 representing NM_030675
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAATCCAGAAAACATCGAAGATGCTTACGTTGCAGTTATTCGTCCAAAGAACACTGCTAGTCTCA
ACTCCCGGGAGTATAGAGCTAAGTCCTATGAAATTTTATTGCATGAAGTTCCCATTGAAGGACAGAAAAA
AAAGCGAAAGAAAGTTTTGCTGGAAACTAAACTTCAAAGCAACAGTGAAATAGCACAAGGCATATTGGAC
TATGTAGTAGAAACTACCAAACCAATTTCTCCTGCAAACCAGGGGATTAAAGGGAAACGAGTGGTGCTGA
TGAGGAAGTTTCCTCTGGACGGAGAGAAGACAGGCAGAGAAGCAGCACTGTTTATCGTGCCATCAGTTGT
CAAAGATAATACTAAATATGCATATACTCCTGGATGCCCAATTTTTTACTGCTTACAAGATATTATGAGA
GTTTGTAGTGAATCCAGTACTCACTTTGCAACACTTACAGCAAGGATGTTAATAGCCTTGGATAAGTGGT
TAGATGAACGTCATGCGCAGTCTCACTTTATTCCAGCTTTATTCCGACCTTCTCCCCTTGAACGGATAAA
GACAAATGTCATAAACCCTGCGTATGCTGCTGAATTAGGCCAGGTAGACAATTCACTACATATGGGCTAT
AGTGCACTAGAAATAAAGAGTAAAATGCTAGCCCTAGAGAAAGCAGACACCTGCATTTACAACCCTTTGT
TTGGATCAGATCTTCAGTATACAAATCGGGTAGATAAAGTGGTAATAAATCCATACTTTGGTCTCGGAGC
TCCAGACTACTCAAAAATCCAAATTCCCAAACAGGAAAAATGGCAGCGAAGCATGAGCAGCGTTGTGGAA
GACAAAGAACGACAGTGGGTTGATGACTTTCCTTTACATCGAAATGCCTGTGAAGGAGATTCAGAATTAC
TGAGCCATCTTCTCGATAAAGGACTTTCAGTCAACCAACTAGATAATGACCACTGGGCACCCATTCATTA
TGCATGCTGGTATGGAAAAGTTGAGGCCACTCGCATATTATTAGAGAAAGGAAAGTGCAATCCAAACCTT
TTAAATGGGCAGCTCAGCTCACCGCTTCACTTTGCTGCTGGAGGCGGCCATGCTGAAATAGTGCAGATCC
TCCTGACTCACCCAGACATTGACAGGCACATAACAGATCAACAAGGAAGATCCCCATTAAATGTTTGTGA
AGAAAACAAACAAAATAACTGGGAAGAAGCTGCAAAATTGTTGAAAGACGCCATTAACAAGCCATATGAA
AAAGTTCGAATCTATAGAATGGATGGATCATACCGTTCTGTTGAACTAAAGCATGGCAATAATACCACAG
CACAGCAGATAATGGAGGGAATGCGGCTCTCTCAGGAAACTCAGCGATATTTCACTATTTGGATCTGTTC
AGAAAATCTTAGTCTTCAGTTCAAGCCTTATCATAAACCCTTGCAACAAGTTCATGACTGGCCAGAAATA
CTTGCTGAATTGACTAATTTGGATCCACAAAGAGAAACACCACAGCTTTTCCTAAGAAGAGATGTGGGAC
TTCCTTTAGAAGTTGAGAAAAAGATTGAAGACCCACTAGCTATTCTTATTCTCTTTGATGAAGCCAGATA
TAATTTACTGAAGGGCTTTTATACAGCTCCTGATGCTAAACTGATAACACTGGCAAGTCTACTGTTACAA
ATAGTTTATGGGAATTATGAGAGTAAAAAGCACAAACAAGGTTTCTTAAATGAAGAAACTCTGAAATCCA
TCGTACCTATTACTAAACTGAAAAGTAAGGCGCCTCACTGGATAAACCGAATACTCCATGAGTACAAGAA
TCTGAGTCTGAGTGAGGGCGTCAGTAAGGAAATGCACCACCTGCAGCGCATGTTCCTACAGAACTGCTGG
GAGATCCCTACGTACGGAGCCGCCTTCTTCACAGGACAGATATTTACAAAGGCAAGCCCAAGCAATCATA
AAGTCATCCCTGTGTATGTAGGAGTGAATATAAAAGGACTTCATCTCCTGAACATGGAAACTAAGGCATT
ACTCATCAGTCTCAAGTACTGTTGCTTTACGTGGCAGCTGGGAGATGCTGGTACTTGTTTTCAAATCCAT
AGTATGGAAAATAAAATGAGCTTTATAGTACACACAAAACAGGCTGGCCTTGTGGTAAAGCTGCTAATGA
AGTTAAATGGACAACTAATGCCCTCTGAAAGAAATTCATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_030675
Insert Size 2211 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030675.3, NP_109600.2
RefSeq Size 6105 bp
RefSeq ORF 2211 bp
Locus ID 79264
UniProt ID Q6S5J6
Cytogenetics 5 2.26 cM
Summary Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. Negative regulator of angiogenesis. Inhibits endothelial proliferation, apoptosis, migration, lumen formation and sprouting angiogenesis in primary endothelial cells. Promotes AKT phosphorylation in a NOTCH-dependent and independent manner, and inhibits ERK1/2 phosphorylation indirectly through activation of the DELTA-NOTCH cascade. Acts in concert with CDH5 to establish and maintain correct endothelial cell polarity and vascular lumen and these effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction, and cell junction stabilization. Plays a role in integrin signaling via its interaction with ITGB1BP1; this prevents the interaction between ITGB1 and ITGB1BP1. Microtubule-associated protein that binds to phosphatidylinositol 4,5-bisphosphate (PIP2)-containing membranes in a GTP-bound RAP1-dependent manner (By similarity). Plays an important role in the maintenance of the intracellular reactive oxygen species (ROS) homeostasis to prevent oxidative cellular damage. Regulates the homeostasis of intracellular ROS through an antioxidant pathway involving FOXO1 and SOD2. Facilitates the down-regulation of cyclin-D1 (CCND1) levels required for cell transition from proliferative growth to quiescence by preventing the accumulation of intracellular ROS through the modulation of FOXO1 and SOD2 levels.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Isoform 1 is also known as Krit1A.
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Documents
Resources
"NM_030675" in other vectors (4)
SKU Description Size Price
MG226187 Krit1 (tGFP-tagged) - Mouse KRIT1 ankyrin repeat containing (Krit1) transcript variant 1, (10ug) 10 ug
$874.00
MR226187 Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1 10 ug
$589.00 $674.00
MR226187L3 Lenti ORF clone of Krit1 (Myc-DDK-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1 10 ug
$974.00
MR226187L4 Lenti ORF clone of Krit1 (mGFP-tagged) - Mouse KRIT1, ankyrin repeat containing (Krit1), transcript variant 1 10 ug
$974.00

file-alt Citations

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