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Lca5 (NM_027448) Mouse Untagged Clone

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SKU
MC220846
Lca5 (untagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $710.00
5 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Lca5
Synonyms 4930431B11Rik; 5730406O13Rik; AV274874; ORF64
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220846 representing NM_027448
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGAAAGAGCCCGAAGTCCAGATATTGAACAAGGAAAAAAAGGAGGCAAACACCCATACTCTCATT
ATTCGTCTGACTTTGGGTCCTCACCACAGTCCTCTGGCCCCTCATCACCTGTCAACACGACGCCATGTGC
CAGTACTAGGGAGAAGAATCCCAAAAGACACTTGTCAGACAACCAAGTGCATCACCCAACCGTTAGGAAA
GTAAGTCCTAAGGCTGTACCCAGCAAAAAGGGCATCCGAGTGGGATTTCGGTCCCAAAGCCTCAACAGAG
AGCCACTTCGGAAAGATCCAGATATCGTAACAAAGCGGGTTCTTTCTGCCAGACTTCTGAAAATCAACGA
GCTGCAGAACGAAGTGTCAGAACTACAGGTCAAGTTAGCCCAGCTGCTCAAAGAGAACAAGGCTTTGAAA
AGCCTTCAGTATCGTCAGGAGAAAGCGCTGAATAAGTTTGAAGATGCGGAGAACGAGATCTCACAACTTA
TACACCGCCATAACAATGAGATTACTGCGCTCAAAGAACGCTTACGAAAATCTCAGGAGAAGGAGCGGGC
GACAGAGAAAAGAGTGAAAGAGACAGAAGGTGAACTATTTAGGACGAAATTTTCCTTACAGAAACTGAAA
AAGATCTCCGAGGCCAGACACCTACCGGAGCGAGACGATTTGGCCAAGAAACTGGTTTCGGCAGAGTTAA
AATTAGATGACACGGAGAGAAAGATTAAGGAGCTATCGAAGAACCTGGAGCTGAGCACGAACAGTTTCCA
GCGGCAGCTGCTCGCTGAAAGGAAAAGAGCATTCGAGGCTTACGATGAAAATAAAGTTCTTCAAAAGGAG
CTGCAGCGGCTCCATCACAAGCTCAAGGAAAAGGAGAAAGAATTGGATATAAAAAACATCTATGCTAATC
GCCTGCCCAAATCCTCTCCAAAGAAAGAGAAAGAAATCGAAAGGAAACATGTATCCTGCCAGAGTGACTT
TACAGACCAGTGTACAAAAGGAGTACAGACCGCTGAAGACTTTGAGCTGGAGGACTTTCCTTTCACAGCC
CAGACAGTTCTCTGCTATGAAAACAGATGGGATGAACCAGAATATCTCTCTTCGTACCTGGAGTACCAGG
ATCTCAACAAACATGGATCAGAGATGCTAAGTTCAGTGTTGGGACAAGAAGGAAAGTATGATGAAGATGA
GGACCCATGCTCTGCAAAGCAGGAGGCCAGGAAGCCTGAGAGTGAATGGGCAAGAGAAGAACTTGATAAG
GTGAAAGGCAAGTCAGCTTTGCTAGGGAGAGCAGAGAAGCTGGCATTGGAAGCTGGGAGATTCCCAACGG
AAAATTATCAAGCTCAGTCTGTCGATAAATTCGAAGATGAGGCAGAAAGACTAAAGACAGAAATGCTCCT
TGCCAAACTGAACGAAATCAACAAAGAACTCCAAGATCCTCAGAACCTCGGGCGGGCCCCTTTGCCATTG
CTGCCCAACTTCGAATCCAAGCTGCACTCTCCAGACAGAAGTACCAGACCTTACTCGTTCCCCGAGTCCT
TAGACCGATCGTTTAATGGACAACACTTGCAGGACTTGAGTTTTTTAACTCCCAGGGGAGAAGGCGGGAG
TCCAGGACCGATCCGAAGTCCAGGACAGATCCGAAGTCCAGCCCCTCTAGATGAGTTTTCATTTGGTAGC
TATGTGCCTTCGTTTGGAAAAACATTAGGGAAGTCAAATCCACCTAGCCAAAAAAGTAGCCTTTTAGATT
TCCAAAGCAACAGTTCTGAAAGCCCGAGTAAAGACAGTCTTGATTTAATGTCGAGGAAAGAGAAAAAGGC
GACTCTGATGGAGCAGCTGTTTGGCCCCAGCGCCAGCAACACCAGTGTCTCCTCCAAGAGCACCGACCCA
CACTTCCCTGCTGCCAGCAGAGGTGACATGGACCCTCTTCATTTCCTCTCTGGGGACAGAAACAGCAGAG
TCCGGGAGCCTGGCGATGAAGAGGAAGACCTTTTCCTCAGGGAAGGAAGAAGTTTTAATCCAAATAGACA
CCGGTTGAAGCACGCAAGCAATAAGCCAACAGTCACGGCCGTTGACTCTGTTGATGAGGACATTGAAGAG
GTTACCCTCAGATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_027448
Insert Size 2115 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027448.2, NP_081724.1
RefSeq Size 4235 bp
RefSeq ORF 2115 bp
Locus ID 75782
UniProt ID Q80ST9
Cytogenetics 9 E2
Summary Involved in intraflagellar protein (IFT) transport in photoreceptor cilia.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) represents the longer transcript and encodes the longer protein (isoform b).
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Documents
Resources
"NM_027448" in other vectors (4)
SKU Description Size Price
MG210151 Lca5 (tGFP-tagged) - Mouse RIKEN cDNA 4930431B11 gene (4930431B11Rik), transcript variant 2 10 ug
$846.00
MR210151 Lca5 (Myc-DDK-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 10 ug
$589.00 $646.00
MR210151L3 Lenti ORF clone of Lca5 (Myc-DDK-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 10 ug
$946.00
MR210151L4 Lenti ORF clone of Lca5 (mGFP-tagged) - Mouse Leber congenital amaurosis 5 (human) (Lca5), transcript variant 2 10 ug
$946.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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