OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Git2 (NM_019834) Mouse Untagged Clone

Be the first to review this product
SKU
MC220773
Git2 (untagged) - Mouse G protein-coupled receptor kinase-interactor 2 (Git2), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $714.00
5 Weeks*
Bulk Requests & Clone Modifications

Frequently Bought Together (3)

CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
TF81001 TurboFectin Transfection Reagent (1 mL in 1 vial)
1 ml in 1 vial
$530.00

Other products for "Git2 or NM_019834"

Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Git2
Synonyms 1500036H07Rik; 5830420E16Rik; 6430510B20Rik; 9630056M03Rik; B230104M05Rik; CAT2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220773 representing NM_019834
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAAGCGGCTCCGGAGCAGCGACGTGTGCGCCGACTGCAACGGGCCTGATCCTTCCTGGGCGTCAG
TAAATAGGGGAACTTTCATATGTGACGAATGTTGCAGTGTCCATCGGAGCCTGGGGCGCCATATCTCCCA
AGTGAGGCATCTTAAACACACAGCATGGCCTCCAACCCTGCTCCAGATGGTTGAGACCTTGTACAACAAT
GGTGCTAACTCTATATGGGAACACTCTCTGCTGGACCCTGCCTCTATCATGAGTGGGAGGCGGAAAGCTA
ACCCACAGGACAAAGTTCACCCCAATAAAGCAGAATTCATCAGAGCCAAGTATCAGATGTTAGCGTTCGT
CCACCGATTGCCCTGCCGCGAGGACGACAGCGTGACGGCCAAAGATCTCAGTAAGCAACTCCATTCAAGT
GTGAGAACAGGGAATCTTGAGACCTGTCTGAGACTGTTGTCTTTAGGAGCCCAAGCCAACTTCTTTCATC
CTGAAAAAGGAAGCACTCCACTCCACGTCGCCTCCAAAGCAGGACAGATCTTACAGGCTGAATTGCTGGC
CGTGTATGGAGCTGACCCAGGCACACAAGATTCTAGTGGGAAGACTCCTGTTGACTATGCAAGGCAAGGA
GGGCACCATGAGTTGGCAGAACGCCTCATAGAAATACAGTACGAGCTGACGGATAGACTGGCCTTCTACC
TCTGCGGCCGGAAACCAGATCATAAAAGCGGACAGCACTTTCTAATTCCTCAGAGGGCAGACAGCCTGGA
TTTGTCTGAGTTGGCAAAAGCTGCGAAGAAGAAACTTCAATCTCTGAGTAATCATTTGTTTGAAGAACTT
GCCATGGATGTGTACGACGAAGTTGACAGGCGAGAGACTGACGCAGTCTGGCTTGCCACGCAGAACCACA
GTACCTTGGTAACCGAGACGACCGTCGTCCCTTTCCTTCCGGTCAACCCTGAGTACTCCTCCACACGAAA
CCAGGGCAGACAGAAATTAGCTCGGTTTAACGCCCACGAGTTTGCTACGCTGGTCATCGACATCCTCAGT
GATGCCAAGAGGAGACAACAGGGCAGTCCTCTGTCCAGGTCAAAAGACAATGTGGAGCTCATATTGAGAA
CAGTCAGTACCCAGCACAGCACTGAGAGCCAAGACAACGACCAGCCAGACTATGACAGTGTGGCCTCCGA
TGAAGACACAGACGTGGAGACGAGGGCAAGCAAGGCAAACCGGCAGAAGCTTCAAACTCTCCAGAGTGAG
AACTCAAGCCTCAGGAGACAGGCCACAGCCAGTGCCTGTCAGGTGCAGACTGGCTCTGACCACAAGGACA
CTGCAAGCCACTCGTCCCTAAAGCGACGGCCATCTGCCCGGGGCAGTAGACCCATGTCCATGTACGAGAC
TGGATCAGGCCAGAAGCCATACCTCCCAATGGGAGAAGCCAGCCACCCTGAGGAGAGCCGGACGAGACTT
CAACCCTTCCCCACGCATATTGGGAGGAGTGCACTTGTGACCTCCTCTTCGTCTCTGCCCTCCTTCCCCT
CCACACTTTCCTGGTCCAGGGACGAAAGCGCCCGAAGGGCCTCCAGACTGGAGAAGCAGAACAGCACCCC
GGAGAGTGATTATGACAACACTGCCTGTGACCCTGAGCCAGACGACACGGGGTCAACCCGGAAGGGACGG
CAGCGGAGCATGCTCTGGCAAGGTGATGGCTTATTGCCAGATACAGCAGAACCCCACTCGGTCCCCAGCC
CCACCCTCCCCAGCACAGAAGACGTCATCCGGAAGACCGAGCAGATCACCAAAAACATCCAGGAGCTCCT
GCGAGCAGCCCAGGAGAACAAGCATGACAGCTATATTCCCTGCTCAGAGAGGATACACGTTGCTGTAACC
GAGATGGCAGCCCTGTTCCCCAAAAAACCCAAGTCTGACACGGTGAGGACTTCCCTCCGCCTACTCACGT
CCAGTGCCTACCGGCTCCAGTCGGAGTGCAGAAAGGCTCTCCCAGGAGACTCCAGCTTACCCACAGATGT
GCAGCTGGTCACACAGCAGGTCATCCAGTGTGCCTATGACATCGCCAAGGCCGCCAAACAGCTGGTCACA
ATCACCACCAAAGAGAACAGCAGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_019834
Insert Size 2127 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019834.3, NP_062808.3
RefSeq Size 5027 bp
RefSeq ORF 2127 bp
Locus ID 26431
UniProt ID Q9JLQ2
Cytogenetics 5 F
Summary GTPase-activating protein for the ADP ribosylation factor family.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_019834" in other vectors (4)
SKU Description Size Price
MG222164 Git2 (tGFP-tagged) - Mouse G protein-coupled receptor kinase-interactor 2 (Git2) transcript variant 1, (10ug) 10 ug
$789.00 $913.00
MR222164 Git2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase-interactor 2 (Git2), transcript variant 1 10 ug
$589.00 $713.00
MR222164L3 Lenti ORF clone of Git2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor kinase-interactor 2 (Git2), transcript variant 1 10 ug
$1,013.00
MR222164L4 Lenti ORF clone of Git2 (mGFP-tagged) - Mouse G protein-coupled receptor kinase-interactor 2 (Git2), transcript variant 1 10 ug
$1,013.00

file-alt Citations

[High-Content siRNA Screen of the Kinome Identifies Kinases Involved in Git2-Induced Mesenchymal-Epithelial Transition]
Cao, Xu, Yang et al
Mol. Biol. (Mosk.) (2017) 51 (4), 696-703 DOI: 10.7868/S0026898417040073
Snippet: cDNA and plasmid transfection. Git2 cDNA was obtained from Origene (#MC220773). Two micro- grams of Git2 cDNA or control plasmids were mixed with 3 mL of FuGENER 6 Transfection Reagent (#E2691;Promega, USA) and incubated for 15 min at room temperature
High-content siRNA screen of the kinome identifies kinases involved in Git2-induced mesenchymal-epithelial transition
Cao, Xu, Yang et al
Molecular Biology (2017)
AI Summary: OriGene's Git2 cDNA (#MC220773) was used for plasmid transfection experiments to study the role of Git2 in mesenchymal-epithelial transition in the referenced paper.
Snippet: cDNA and plasmid transfection. Git2 cDNA was obtained from Origene (#MC220773). Two micro- grams of Git2 cDNA or control plasmids were mixed with 3 mL of FuGENER 6 Transfection Reagent (#E2691;Promega, USA) and incubated for 15 min at room temperature ... Cite SaveSaving
Showing 1-2 of 2 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products