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Ikbke (BC037446) Mouse Untagged Clone

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SKU
MC220696
Ikbke (untagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $699.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ikbke
Synonyms Ikki, IKK-i
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC037446
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGTACCACTAACTACCTGTGGCATACTGATGACCTGCTAGGGCAGGGGGCCACTGCCAGTGTGT
ACAAGGCCCGAAACAAGAAATCCGGGGAGGTGGTTGCTGTAAAGGTCTTCAACTCAGCCAGCTATCGGCG
ACCTCCTGAGGTTCAGGGAGGCAGCCGGCAGAAGGTGCTAATCATGGAGTACTGCTCCAGTGGGAGCCTG
CTGAGCGTGCTGGAAGACCCTGAGAACACGTTCGGGCTTTCTGAAGAGGAGTTCCTAGTGGTGCTGCGCT
GTGTGGTGGCTGGCATGAACCACCTGCGGGAGAATGGCATTGTCCATCGGGACATCAAACCTGGGAACAT
CATGCGCCTGGTGGGCGAGGAGGGGCAGAGCATCTATAAGCTGTCTGACTTCGGGGCTGCCCGCAAGCTG
GACGATGATGAGAAGTTTGTTTCTGTCTATGGTACAGAGGAATACCTGCACCCTGACATGTATGAGCGTG
CAGTGCTGCGCAAACCCCAGCAAAAGGCATTTGGTGTGACTGTGGATCTCTGGAGTATTGGGGTGACCCT
GTACCACGCAGCCACAGGCAGTCTGCCCTTCATCCCCTTCGGTGGGCCCCGGCGCAACAAAGAGATCATG
TACAGAATCACCACAGAGAAGCCAGCCGGGGCCATTTCAGGGACTCAGAAGCAGGAAAATGGTCCCTTGG
AGTGGAGCTACAGCCTCCCCATCACCTGTAGACTGTCCATGGGGCTGCAGAACCAGCTGGTGCCCATCCT
GGCCAACATCCTGGAGGTGGAAGAGGATAAGTGCTGGGGCTTTGATCAGTTCTTCGCGGAGACCAGTGAC
ATTCTGCAGCGAACGGTCATCCACGTCTTTTCCCTACCCCAGGCCGTTTTGCATCATGTCTACATCCACG
CCCACAACACGATTGCCATCTTTTTGGAGGCTGTATATGAGCAGACCAACGTGACCCCCAAACACCAGGA
GTACCTCTTCGAGGGTCACCCTTGTGTCCTTGAGCCAAGCCTCTCAGCCCAGCACATCGCCCACACAGCT
GCCAGCAGCCCTCTAACTCTGTTCAGCATGTCCAGCGACACACCTAAGGGGCTGGCCTTCAGGGACCCTG
CTCTGGATGTCCCAAAGTTCGTCCCTAAGGTTGACCTACAGGCCGATTACAGCACAGCTAAGGGGGTGCT
GGGCGCTGGCTACCAGGCCCTGTGGCTGGCGCGGGTCCTGCTGGATGGACAGGCGTTGATGCTTCGGGGG
TTACATTGGGTCCTGGAGGTGCTTCAGGACACGTGCCAGCAGACACTGGAGGTCACACGGACAGCCCTCC
TCTACCTCGGCAGCAGCCTGGGCACTGAAAGGTTCAGCAGTGGATCGGGGATGCCTGACGTCCAGGAACG
AAAGGAGGCCACAGAGCTAAGAACCAGGCTGCAGACTCTCTCAGAGATCCTGTCTAAATGTTCCCACAAT
GTCACAGAAACCCAAAGGAGCCTGAGCTGTCTGGGTGAAGAGCTTTTAAAGAACCGGGACCAGATTCATG
AGGATAACAAAAGTATCCAGAAGATTCAGTGTTGTTTGGACAAGATGCACTTCATCTACAAACAGTTCAA
GAAATCCAGGATGAGGCCAGGGCTCAGCTACAATGAGGAGCAGATCCACAAGCTGGATAAGGTAAATTTC
AGTCATCTAGCCAAGAGGCTGCTGCAGGTGTTCCAGGAGGAGTGTGTGCAGACGTATCAGGTGTCGCTGG
TCACACACGGCAAGCGGATGAGGCAGGTGCAGAGGGCCCAGAACCACCTGCATCTCATTGGCCACTCTGT
GGCCACCTGTAACTCGGAAGCCCGGGGAGCCCAGGAGAGTCTGAACAAGATCTTTGATCAGCTCCTTCTG
GACAGAGCTTCCGAACAGGGAGCTGAGGTGTCACCGCAACCTATGGCTCCTCATCCCGGCCCTGATCCGA
AGGACCTGGTCTTCCACATGCAGGAGCTTTGTAATGATATGAAGCTATTGGCCTTTGATCTCCAGGACAA
CAACCGACTCATCGAACGGTTACATAGAGTTCCATCGGCACCAGATGTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC037446
Insert Size 2082 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC037446, AAH37446
RefSeq Size 2931 bp
RefSeq ORF 2081 bp
Locus ID 56489
Cytogenetics 1 E4
Summary Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1.UniProtKB/Swiss-Prot Function
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"BC037446" in other vectors (4)
SKU Description Size Price
MG210080 Ikbke (tGFP-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145) 10 ug
$789.00 $835.00
MR210080 Ikbke (Myc-DDK-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145) 10 ug
$589.00 $635.00
MR210080L3 Lenti ORF clone of Ikbke (Myc-DDK-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145) 10 ug
$935.00
MR210080L4 Lenti ORF clone of Ikbke (mGFP-tagged) - Mouse inhibitor of kappaB kinase epsilon (cDNA clone MGC:46849 IMAGE:3980145) 10 ug
$935.00

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