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Dbf4 (NM_013726) Mouse Untagged Clone

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SKU
MC220248
Dbf4 (untagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $680.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Dbf4
Synonyms AA545217; Ask
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220248 representing NM_013726
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTCGAGACCATGAGGATCCACAGCAAAGCACCTCTCCCGGGTGGCATCCAAGACAGAAATGAAA
AGAATCGACCATCCTTGAAATCCCTGAAGGCTGATAATAGACTGGAAAAATCCAAATATAAGCCCCTTTG
GGGAAAAATATTTTACCTTGACTTGCCTTCTATCACCATATGTGAGAAACTACAAAAAGATATAAAGGAA
CTCGGAGGGCGAGTTGAAGAATTTCTCAGCAAAGATATCAGTTATTTTGTTTCAAATAAAAAGGAAGCAA
AGTATGCACAGACATTGGGTCGCGTTTCTCCTGTACCAAGTCCAGAATCTGCATATACTGCAGAAACCAC
CTCTCCTCATCCCAGCCATGATGGAAGTTCCTTTAAGTCTCAAGACAGAGTATGTTTGAGCCGAGGAAAA
TTGTTAGCTGAAAAAGCTGTGAAGGATCATGATTTTATTCCTGCTAATAGTATATTATCAAATGCCTTAT
CATGGGGAGTAAAGATTCTTCATATTGATGACATTCGATACTACATTGAACAGAAGAAGAAAGCACTGAG
TGCACTCAAGAAATCCAGTGCTTCTGGGAAGGATGCGGGGAAAAAAGCTGGTCCTGGAATACAAAAGACA
AGAACAGGGAGACTCAAGAAGCCTTTTTTAAAGGTTGAAGATGTGAACCGGTGCTACAGGCCATTTTACC
TTCAGCTCCCCAGTCTGCCGTGTATAAACTACTTTCTTCAGAAGCCCTGCAGTCCGTTTGATATAGAGAA
GTCATCCAGCGTCCAGAAACAAGCTCAGCCCAAGCTCAGAATTAATATGGACGGCGATAAATGTGGAACC
CCAGTTCAACTCCAGTTGAAGGAAAAGAGAAAAAAAGGATATTGTGAATGTTGCTTACAGAAATACGAAG
ATCTCGAAACTCACCTTCTGAGTGAGAAACACAGGAACTTTGCACAGAGTAACCAGTATCAAGTTGTTGA
TGATATTGTATCTCAGTTAGTTTTCGATTTTGTGGAATATGGAAGAGACACACCTCAAAAGAAAAGAATA
AGATACAGTGTCGGGTCCCTTTCTTCTGTTTCTGCAAATGTCCTGAAAAACACTGCACCGAAGGAGAAGC
CACTCTTGGAGCCCAATTTCCAGAAGGACGTGGGCGAAAGCAGTGGGCACCTGCTCAAGCCGAACTCTCA
GTATGAAGAAACCCAGAAACCTGAAGAAAAGCATGGGTTTGCTTCAGAGCCCACAACCTATTCTTCAGCT
GGATTGAAAGGATGTGACAGGAAACCAGTGAGCATGTTCAATGCAAGTGAGCCTGACCCAGAGCAGGAGT
ATGCACAGCTACCTCTGCGTGACAGCACACCGGAACACCAAGTGACCGAGGGTAGAAATGACGGAGAACA
GAGAGTAGACCCTGCTCCAGGCGTGTCGCAGTCATGTGGACAAGTTTCTCATCTCAGTACAGAAAGTAAT
CTACCCCAACCACAGTTAGCTGCAGACATTACACAGCTTTCAGCAAAAGATCTGCAAGAAAAGGGCTTTC
ATGTTGTGATAGGTCATGCTTCTGACCTGGTGGCATTAAATACATCAAAAGAGCAGCTAACAATGAAGGC
GAGAACTCCACCCTGTAGTCCCCAGGAGCCTCATGAGTGTGACACTGAGAACATGGAGAATTTGCCTTGT
GGTAAAATCCAGCGGAAAGTGAGAATGCTATTAGGGCAACAGAAAGCAAATGCTGAACCGAGTGCTGAAT
TGGATAAAAAGAGAACTGAGTATCTTCCTGCACATGAAGACAGAACTTGTGGGTCACCAGTACAGTCTCT
ATTGGACCTTTTTCAGACAAGCGAGGAGAAATCAGAATTTTTGGGTTTTACAGGCTACACTGAAAACAGT
GGAATATGTGATGTTTTAGATATTTGGGAGGAAGAAAATTCAAGTACCCTGTTGTCAACGTTTTTTTCTT
CCCCTTCAACTTCTGCATTCGTTGGATTTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_013726
Insert Size 1992 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013726.3, NP_038754.1
RefSeq Size 2444 bp
RefSeq ORF 1992 bp
Locus ID 27214
UniProt ID Q9QZ41
Cytogenetics 5 A1
Summary Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role DNA in replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
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Documents
Resources
"NM_013726" in other vectors (4)
SKU Description Size Price
MG222369 Dbf4 (tGFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4) transcript variant 1, (10ug) 10 ug
$817.00
MR222369 Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 10 ug
$289.00 $617.00
MR222369L3 Lenti ORF clone of Dbf4 (Myc-DDK-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 10 ug
$917.00
MR222369L4 Lenti ORF clone of Dbf4 (mGFP-tagged) - Mouse DBF4 homolog (S. cerevisiae) (Dbf4), transcript variant 1 10 ug
$917.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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