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Edem1 (NM_138677) Mouse Untagged Clone

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SKU
MC220111
Edem1 (untagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $669.00
5 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Edem1
Synonyms A130059K23Rik; EDEM; mKIAA0212
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220111 representing NM_138677
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAATGGCGAGCGCTTGTCCTGGGGCTGGTGCTGCTGCGCCTCGGCCTCCACGCGGTGCTCTGGTTGG
TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGGTTTCCGCTCAGCTTCGGCTTCCAGCGCTTGAG
GGACCCCGACGGCTCGGGGCCAGTTGGTCCGCCCGGAGGCCCGGCCTGGCTTCATCGGCCAAGGCGCGGA
ACGGAGGGGCGGCTTGAGACCCCGCCAGAGCCGGGACCGACGCCGGGACCGGGCGTGTGCGGCCCGGCGC
ACTGGGGCTATGCCCTGGGCGGCGGTGGCTGCGGCCCGGACGAGTACGAGCGGCGCTACAGCGGCGCCTT
CCCGCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCGCGGGGCATGTTCGTCTTCGGCTATGACAACTAC
ATGGCGCACGCCTTTCCGCAGGACGAGCTCAACCCCATCTACTGCCGCGGCCGGGGGCCTGACCGCGGAG
ACCCTTCCAATCTGAACATCAATGATGTCCTAGGGAATTACTCTCTGACTCTGGTTGATGCCTTGGATAC
ATTGGCAATAATGGGAAATTCATCCGAGTTCCAGAAGGCAGTCAAGTTAGTGATCAACACTGTTTCATTT
GACAAAGATTCCACAGTCCAGGTCTTCGAAGCTACGATAAGGGTTCTGGGAAGCCTCCTTTCTGCTCACA
GAATAATAACTGATTCCAAACAGCCCTTTGGTGACATGACAATTGAGGATTATGATAATGAATTGTTGTA
CATGGCCCATGACTTGGCTGTGCGGCTCCTTCCAGCCTTTGAAAACACCAAGACAGGGATCCCCTATCCT
CGGGTGAATCTGAAGACAGGTGTTCCTCCTGACAGCAATAATGAGACCTGCACAGCGGGGGCCGGTTCTC
TCCTGGTGGAATTTGGGATTCTGAGCCGACTGCTGGGGGATTCCACTTTTGAGTGGGTTGCCAGACGAGC
TGTGAAAGCCCTCTGGAACTTGCGGAGCAACGATACAGGATTATTAGGCAATGTTGTGAACATCCAGACA
GGCCATTGGGTTGGAAAGCAGAGTGGCTTGGGTGCTGGGCTGGATTCCTTCTATGAGTACCTCTTGAAAT
CTTACATTCTTTTTGGAGAAAAAGAAGATCTAGAGATGTTTAATGCTGCGTATCAGAGCATCCAGAGCTA
CCTGCGAAGAGGCCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCACCGCTCTACGTCAACGTGAACATG
TTCAGTGGGCAGCTCATGAACACCTGGATTGACTCGCTGCAGGCTTTCTTCCCTGGACTGCAGGTTCTGA
TAGGGGATGTGGAAGATGCCATCTGCCTACACGCCTTCTACTATGCCATATGGAAGCGGTACGGGGCCCT
CCCTGAGCGCTATAACTGGCAACTCCAGGCCCCTGATGTTCTCTTCTACCCTCTGAGACCAGAGCTAGTG
GAGTCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGACATTCTGC
AGAGTCTTGAAAAATACACAAAAGTCAAATGTGGATATGCTACGCTGCATCACGTCATAGACAAGTCTAA
AGAAGACCGGATGGAAAGCTTCTTTCTCAGCGAGACTTGCAAATACTTGTATCTGCTATTTGATGAAGAG
AATCCAGTACACAAATCTGGAACCAGATACATGTTTACAACTGAGGGCCATATCATATCTGTGGACAAAC
GTCTTCGGGAATTGCCTTGGAAGGAATTCTTCTCTGAAGATGGGGAGCGGGACCAAGAGGAAAAGTTTGT
ACACAGACCTAAGTCTCAGGAGCTCAGAGTCATTAACTCCAGTTCTAATTGTAATCGTGTTCCTGATGAG
AGGAGATACTCCTTGCCCTTAAAGAGCATCTACATGCGCCAGATCGACCAGATGGTTGGCTTGATTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_138677
Insert Size 1959 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_138677.2, NP_619618.1
RefSeq Size 5858 bp
RefSeq ORF 1959 bp
Locus ID 192193
UniProt ID Q925U4
Cytogenetics 6 E2
Summary Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.UniProtKB/Swiss-Prot Function
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"NM_138677" in other vectors (4)
SKU Description Size Price
MG209777 Edem1 (tGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) 10 ug
$807.00
MR209777 Edem1 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) 10 ug
$289.00 $607.00
MR209777L3 Lenti ORF clone of Edem1 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) 10 ug
$907.00
MR209777L4 Lenti ORF clone of Edem1 (mGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) 10 ug
$907.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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