Limk1 (NM_010717) Mouse Untagged Clone

SKU MC220064

Limk1 (untagged) - Mouse LIM-domain containing, protein kinase (Limk1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Limk1
• Synonyms: KIZ-1; Limk; LIMK-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC220064 representing NM_010717; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGTTGACGCTACTTTGTTGCACCTGGAGGGAAGAACGTATGGGAGAGGAAGGAAGCGAGTTGCCTG; TGTGTGCGAGCTGTGGCCAGAGGATCTATGACGGCCAGTACCTCCAGGCCCTGAATGCTGACTGGCATGC; AGACTGCTTCAGGTGCTGCGAGTGTAGCGTCTCCCTTTCACACCAGTACTACGAGAAGGATGGACAGCTC; TTCTGCAAGAAGGACTACTGGGCCCGCTATGGCGAGTCTTGCCACGGGTGCTCCGAGCACATCACCAAAG; GGCTGGTCATGGTGGCTGGGGAGCTGAAGTACCACCCTGAGTGTTTCATCTGCCTCGCCTGTGGAAACTT; CATTGGCGATGGGGACACCTACACACTGGTGGAGCACTCCAAGCTGTACTGTGGCCAGTGCTACTACCAG; ACCGTGGTAACTCCAGTCATCGAACAGATCCTACCTGACTCACCCGGCTCCCACCTGCCCCACACAGTCA; CCCTCGTGTCTATCCCAGCCTCTGCCCATGGCAAACGAGGCCTGTCTGTCTCCATCGACCCCCCTCACGG; CCCACCAGGATGTGGCACGGAGCATTCGCACACTGTCCGAGTCCAAGGAGTGGACCCAGGCTGCATGAGC; CCAGATGTGAAGAATTCCATCCACGTTGGAGACCGGATCTTGGAAATCAATGGCACGCCCATCCGGAATG; TGCCGCTGGACGAGATCGACTTGCTGATCCAGGAGACCAGCCGCCTGCTCCAGCTGACCCTCGAGCACGA; CCCCCATGACTCACTGGGCCATGGGCCCGTGTCGGATCCCAGCCCCCTGAGCTCCCCGGTGCACACTCCC; AGCGGACAGGCAGCCAGCTCTGCCCGGCAGAAACCTGTCTTGAGGAGCTGCAGTATTGACACGTCCCCGG; GTACCAGCTCACTAGCCTCTCCAGCCTCCCAGCGCAAGGACCTGGGTCGCTCCGAATCCCTCCGAGTGGT; CTGCCGACCACACCGAATCTTCCGGCCATCTGATCTCATCCATGGGGAAGTGCTGGGCAAGGGCTGTTTT; GGCCAGGCCATCAAGGTGACACACCGAGAAACAGGCGAGGTGATGGTGATGAAGGAACTTATCCGGTTTG; ATGAGGAGACCCAGAGGACATTCCTCAAGGAGGTAAAGGTCATGCGCTGCCTGGAGCACCCTAACGTGCT; CAAGTTCATCGGAGTGCTCTACAAGGACAAGCGGCTAAACTTCATCACAGAGTACATCAAGGGCGGCACC; CTGCGGGGCATCATCAAGAACATGGACAGCCAGTACCCGTGGAGTCAGAGGGTCAGCTTTGCCAAGGACA; TTGCATCAGGGATGGCCTACCTCCATTCGATGAACATCATCCACCGAGACCTCAACTCCCACAACTGTCT; GGTCCGTGAGAATAGGAACGTGGTGGTGGCCGACTTTGGGCTGGCTCGACTCATGATCGATGAAAAGAAT; CAGTCTGAAGACTTACGCAGCCTTAAGAAGCCGGACCGCAAGAAGCGGTACACAGTGGTGGGCAACCCCT; ACTGGATGGCGCCAGAGATGATCAATGGTCGTAGTTATGATGAAAAGGTGGACGTGTTTTCCTTTGGAAT; TGTCCTCTGTGAGATTATCGGGCGTGTGAATGCAGACCCTGACTATCTGCCCCGCACTATGGACTTTGGC; CTCAATGTAAGGGGTTTCCTGGACCGCTACTGTCCACCAAACTGTCCCCCAAGTTTCTTCCCCATCACTG; TGCGCTGCTGTGACCTGGATCCTGAAAAGAGACCCTCTTTTGTAAAGCTGGAACAATGGCTAGAAACACT; TCGCATGCACTTGTCTGGTCACCTGCCACTGGGCCCACAACTGGAACAACTGGAGCGGGGCTTCTGGGAG; ACCTACCGGCGGGGCGAGAGCTCACTGCCTGCCCACCCCGAGGTCCCTGACTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_010717
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_010717.3, NP_034847.1
• RefSeq Size: 3337 bp
• RefSeq ORF: 1944 bp
• Locus ID: 16885
• UniProt ID: P53668
• Cytogenetics: 5 G2
• Summary: This gene encodes a member of the LIM kinase family of proteins. This protein is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein also stimulates axon growth and may play a role in brain development. Homozygous knockout mice for this gene exhibit reduced bone mass, abnormal neuronal morphology and altered synaptic function. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]; Transcript Variant: This variant (1) encodes the longer isoform (1).