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D7Ertd443e (NM_001081331) Mouse Untagged Clone

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SKU
MC220009
D7Ertd443e (untagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $660.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol D7Ertd443e
Synonyms 4933400E14Rik; FATS; Gm497
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC220009 representing NM_001081331
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATATCACCTGTGGTCATTTCCCGGCTGATTGATGAAAAGAAATCTATGGAAAATGGAGCAATATTGC
CACAAGCCATTGCTCAGCCTCAGCTGTGTCCCACAAAGCCAGCTTTGGCCAGGCGCGATGGAGTGAGCAT
GCACAGGCGATTTGCTTTATCTCCAGACAGACTGGGAATTTTGACTCCATCAGATGATCAAGGACTGGAG
ACAGAACCGCTGTCTACAGGGGACAATCTAGGCAAAGGTTCCCACAGCGGTTTCTCGTCTATCACCATTA
CTGCCCGACGTGTGGGCCCTCCAGCCAGCAGCTTGGTATGGGACACTTTTAGGGACCCACTGTGTCCCAA
GTGCAAAGCCAAGGATGCTCTGTTCCAAGAGCCCCCAGTTCTGGCTGGTGATGCCCATCTCTGTCAGCAC
AATAGACCCTTCACCTGTACAGAGTCCCCCAGCAATGGCTCCGTAGAGGGGATGAAGGTTTTCCAGGCTC
ACTCAAGGCTGAGTGCAAGGCAGGACTACTGGGTCACCCACACGAATGACAATGAGGACAGTTTTTCTTC
AGACAATTCACCATCGAGAAAAGTCCCACTGGTGTTCAGTTCTTGTGTCCACTTCCGGGTGTCTCAGCAG
TGTCCCAATGCCATTTACTATTTGGACAAGTCTCTTTCTGTTCCCCTTGAGCGACCTCAAATTGCTAGCC
CCAAAATGCACAGGTCTGTCCTGTCTCTCAGCCTCCGCTGTAGTTCCCACCAGTTAACAGCAGATGGAGT
AGACAGCTCAGCTAATGGAGAGCCAATCAGCACAGCTCTATCACAGGAACTCTCGGAGGGAAAGCAGGAC
CTCCTAGGTCCACAATGGGGCCAGCCACAAGGTGGTCACTGGAAGGAGAGCCCAGCATTGGTGCCAGTAC
ATTTGGGGAGTGGCACATGCCCTCGGACTGGCTCCCCTCCACTGGAAAATGTCAAATTTGCAGACGTGGG
AAGGAACCAGGTCCCAGTACGAAAGGAGAAAGAGGACCATGCAACATGTACCAGTAGCAGCCACACCAAC
CAACTCTCCATCCATATTCCCGGCTGGAGTTACAGGGCTGAAACAAAAGTACTTTCTGGAAGCAAGAAGC
AGCAGCAAGAAGCACAAAGAACTCTTCCTGCTTTCCCAGTGGGACAGAAGACCATTAAACACTTCCCTCC
TGAAGGGGACAGTTCACCAAGCAGTGATGGTCAACCTAGCATCCTTTCAGAGTCCAATGAGAGGCAACAC
CCATACTTCATGATACCCAGAGTCCCTCTGCCTGGGTTTTATTGCCCTTTACAAACTGGGTGTGCCTCTC
TGCAGGAAGATGGTGCTGTTCAGATAGAAACACACTTCCCCAAAGATTACACGTGCTGTGACTTGGTTGT
AAAACTAAAGGAATGTGAGAAGAACGAAGACCCCACTGTCACCCCTGAGCCCTCACCAGCAACCCCCTCT
CCATCAACCCCAGAGGGAGCACAGAGCTCTGACCCATCAGAAGATAGTTACGAGCCTCTGCTGGCAAGTT
CTATGACTTTGCAGGAAGCACTGGAAGTCCATAGACCTCAGTTCATTTCTCGCTCACAAGAACGGCTGCA
AAAGCTTAAGCGCATGGTACAGCAAAGGAAAACCCAACAAAAGGAGAGCCTGGGGCAGAAGCAGAGCCTA
CTTCCTGTCCGCGCCAACAAGAAGCAGTTCACCATTCCCCATCCTCTTAGTGATAACTTGTTCAAACCCA
AAGAAAGATGCATTTCAGAGAAGGAGATGCATATGAGATCTAAAAGAATCTATAATAATTTGCCGGAAGT
TAAAAAGAAAAAAGAAGAGCAGAAGAAAAGAATGATCTTACAGAGTAACCGGCTCCGAGCAGAAGTCTTC
AAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCAGTTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001081331
Insert Size 1935 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081331.1, NP_001074800.1
RefSeq Size 2425 bp
RefSeq ORF 1935 bp
Locus ID 71007
UniProt ID D2J0Y4
Cytogenetics 7 78.4 cM
Summary Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage (PubMed:20843368, PubMed:20154723, PubMed:24240685). Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response (PubMed:24240685). Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (PubMed:20154723). May have a role in the assembly of primary cilia (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001081331" in other vectors (4)
SKU Description Size Price
MG215023 D7Ertd443e (tGFP-tagged) - Mouse DNA segment Chr 7 ERATO Doi 443 expressed (D7Ertd443e), (10ug) 10 ug
$799.00
MR215023 D7Ertd443e (Myc-DDK-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$289.00 $599.00
MR215023L3 Lenti ORF clone of D7Ertd443e (Myc-DDK-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$899.00
MR215023L4 Lenti ORF clone of D7Ertd443e (mGFP-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$899.00

file-alt Citations

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