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D7Ertd443e (NM_001081331) Mouse Tagged ORF Clone

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SKU
MG215023
D7Ertd443e (tGFP-tagged) - Mouse DNA segment Chr 7 ERATO Doi 443 expressed (D7Ertd443e), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $799.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D7Ertd443e
Synonyms 4933400E14Rik; FATS; Gm497
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215023 representing NM_001081331
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATATCACCTGTGGTCATTTCCCGGCTGATTGATGAAAAGAAATCTATGGAAAATGGAGCAATATTGC
CACAAGCCATTGCTCAGCCTCAGCTGTGTCCCACAAAGCCAGCTTTGGCCAGGCGCGATGGAGTGAGCAT
GCACAGGCGATTTGCTTTATCTCCAGACAGACTGGGAATTTTGACTCCATCAGATGATCAAGGACTGGAG
ACAGAACCGCTGTCTACAGGGGACAATCTAGGCAAAGGTTCCCACAGCGGTTTCTCGTCTATCACCATTA
CTGCCCGACGTGTGGGCCCTCCAGCCAGCAGCTTGGTATGGGACACTTTTAGGGACCCACTGTGTCCCAA
GTGCAAAGCCAAGGATGCTCTGTTCCAAGAGCCCCCAGTTCTGGCTGGTGATGCCCATCTCTGTCAGCAC
AATAGACCCTTCACCTGTACAGAGTCCCCCAGCAATGGCTCCGTAGAGGGGATGAAGGTTTTCCAGGCTC
ACTCAAGGCTGAGTGCAAGGCAGGACTACTGGGTCACCCACACGAATGACAATGAGGACAGTTTTTCTTC
AGACAATTCACCATCGAGAAAAGTCCCACTGGTGTTCAGTTCTTGTGTCCACTTCCGGGTGTCTCAGCAG
TGTCCCAATGCCATTTACTATTTGGACAAGTCTCTTTCTGTTCCCCTTGAGCGACCTCAAATTGCTAGCC
CCAAAATGCACAGGTCTGTCCTGTCTCTCAGCCTCCGCTGTAGTTCCCACCAGTTAACAGCAGATGGAGT
AGACAGCTCAGCTAATGGAGAGCCAATCAGCACAGCTCTATCACAGGAACTCTCGGAGGGAAAGCAGGAC
CTCCTAGGTCCACAATGGGGCCAGCCACAAGGTGGTCACTGGAAGGAGAGCCCAGCATTGGTGCCAGTAC
ATTTGGGGAGTGGCACATGCCCTCGGACTGGCTCCCCTCCACTGGAAAATGTCAAATTTGCAGACGTGGG
AAGGAACCAGGTCCCAGTACGAAAGGAGAAAGAGGACCATGCAACATGTACCAGTAGCAGCCACACCAAC
CAACTCTCCATCCATATTCCCGGCTGGAGTTACAGGGCTGAAACAAAAGTACTTTCTGGAAGCAAGAAGC
AGCAGCAAGAAGCACAAAGAACTCTTCCTGCTTTCCCAGTGGGACAGAAGACCATTAAACACTTCCCTCC
TGAAGGGGACAGTTCACCAAGCAGTGATGGTCAACCTAGCATCCTTTCAGAGTCCAATGAGAGGCAACAC
CCATACTTCATGATACCCAGAGTCCCTCTGCCTGGGTTTTATTGCCCTTTACAAACTGGGTGTGCCTCTC
TGCAGGAAGATGGTGCTGTTCAGATAGAAACACACTTCCCCAAAGATTACACGTGCTGTGACTTGGTTGT
AAAACTAAAGGAATGTGAGAAGAACGAAGACCCCACTGTCACCCCTGAGCCCTCACCAGCAACCCCCTCT
CCATCAACCCCAGAGGGAGCACAGAGCTCTGACCCATCAGAAGATAGTTACGAGCCTCTGCTGGCAAGTT
CTATGACTTTGCAGGAAGCACTGGAAGTCCATAGACCTCAGTTCATTTCTCGCTCACAAGAACGGCTGCA
AAAGCTTAAGCGCATGGTACAGCAAAGGAAAACCCAACAAAAGGAGAGCCTGGGGCAGAAGCAGAGCCTA
CTTCCTGTCCGCGCCAACAAGAAGCAGTTCACCATTCCCCATCCTCTTAGTGATAACTTGTTCAAACCCA
AAGAAAGATGCATTTCAGAGAAGGAGATGCATATGAGATCTAAAAGAATCTATAATAATTTGCCGGAAGT
TAAAAAGAAAAAAGAAGAGCAGAAGAAAAGAATGATCTTACAGAGTAACCGGCTCCGAGCAGAAGTCTTC
AAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCAGTT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215023 representing NM_001081331
Red=Cloning site Green=Tags(s)

MISPVVISRLIDEKKSMENGAILPQAIAQPQLCPTKPALARRDGVSMHRRFALSPDRLGILTPSDDQGLE
TEPLSTGDNLGKGSHSGFSSITITARRVGPPASSLVWDTFRDPLCPKCKAKDALFQEPPVLAGDAHLCQH
NRPFTCTESPSNGSVEGMKVFQAHSRLSARQDYWVTHTNDNEDSFSSDNSPSRKVPLVFSSCVHFRVSQQ
CPNAIYYLDKSLSVPLERPQIASPKMHRSVLSLSLRCSSHQLTADGVDSSANGEPISTALSQELSEGKQD
LLGPQWGQPQGGHWKESPALVPVHLGSGTCPRTGSPPLENVKFADVGRNQVPVRKEKEDHATCTSSSHTN
QLSIHIPGWSYRAETKVLSGSKKQQQEAQRTLPAFPVGQKTIKHFPPEGDSSPSSDGQPSILSESNERQH
PYFMIPRVPLPGFYCPLQTGCASLQEDGAVQIETHFPKDYTCCDLVVKLKECEKNEDPTVTPEPSPATPS
PSTPEGAQSSDPSEDSYEPLLASSMTLQEALEVHRPQFISRSQERLQKLKRMVQQRKTQQKESLGQKQSL
LPVRANKKQFTIPHPLSDNLFKPKERCISEKEMHMRSKRIYNNLPEVKKKKEEQKKRMILQSNRLRAEVF
KKQLLDQLLQRNAV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001081331
ORF Size 1932 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001081331.2
RefSeq Size 2425 bp
RefSeq ORF 1935 bp
Locus ID 71007
UniProt ID D2J0Y4
Cytogenetics 7 78.4 cM
Summary Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage (PubMed:20843368, PubMed:20154723, PubMed:24240685). Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response (PubMed:24240685). Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (PubMed:20154723). May have a role in the assembly of primary cilia (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001081331" in other vectors (4)
SKU Description Size Price
MC220009 D7Ertd443e (untagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2, (10ug) 10 ug
$660.00
MR215023 D7Ertd443e (Myc-DDK-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$289.00 $599.00
MR215023L3 Lenti ORF clone of D7Ertd443e (Myc-DDK-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$899.00
MR215023L4 Lenti ORF clone of D7Ertd443e (mGFP-tagged) - Mouse DNA segment, Chr 7, ERATO Doi 443, expressed (D7Ertd443e), transcript variant 2 10 ug
$899.00

file-alt Citations

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