Cpne5 (NM_153166) Mouse Untagged Clone

SKU MC219372

Cpne5 (untagged) - Mouse copine V (Cpne5), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Cpne5
• Synonyms: A830083G22Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC219372 representing NM_153166; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGCAACCCGAGGACATGGCGTCGCTCAGCGAGTTCGACTCCTTGGCGGGCAGCATCCCGGCTACCA; AGGTGGAGATCACGGTGTCATGCAGGAACCTCTTGGACAAAGACATGTTTTCCAAGTCTGACCCACTGTG; TGTCATGTACACTCAAGGGATGGAGAACAAGCAATGGCGAGAGTTCGGACGCACCGAGGTCATAGATAAC; ACGCTCAACCCGGACTTCGTGCGCAAGTTCATTGTGGATTACTTCTTTGAGGAGAAGCAGAACCTCCGCT; TTGATCTCTACGATGTTGACTCCAAGAGCCCGGATCTATCTAAACACGACTTCCTGGGCCAGGCCTTCTG; TACCCTTGGAGAAATTGTAGGGTCCTCTGGGAGCCGTCTGGAGAAGCCTCTCACGATAGGAACATTCAGC; CTCAACTCCCGGACAGGCAAGCCTATGCCCGCCGTGTCTAACGGTGGTGTCCCAGGGAAGAAATGTGGTA; CCATCATCCTGTCTGCAGAGGAACTGAGCAACTGCAGGGATGTGGCCACAATGCAGTTCTGTGCCAACAA; GCTGGATAAGAAAGATTTCTTCGGGAAATCTGACCCCTTCTTGGTGTTCTACAGGAGCAATGAAGACGGA; ACGTTCACCATCTGTCACAAGACGGAGGTCATGAAGAACACCCTCAACCCGGTGTGGCAAACCTTCTCCA; TCCCCGTGAGGGCTCTGTGCAACGGAGACTATGACAGGACCATCAAGGTGGAGGTGTATGACTGGGATCG; TGACGGCAGCCATGACTTCATTGGGGAGTTCACCACCAGCTACCGGGAGCTGGCACGTGGCCAGAGTCAG; TTCAACATCTATGAGGTGATAAATCCAAAAAAGAAAATGAAGAAAAAGAAATACGTGAATTCTGGCACGG; TCACCCTGCTTTCCTTTGCGGTAGAGTCAGAGAGTACCTTCCTGGATTACATCAAAGGAGGGACGCAGAT; CAACTTCACCGTGGCCATTGACTTCACTGCCTCTAATGGGAATCCCTCACAGTCCACGTCCCTCCACTAC; ATGAGTCCCTACCAGCTGAACGCCTACGCGCTGGCGCTGACCGCCGTCGGAGAGATCATTCAGCATTATG; ACAGTGACAAGATGTTCCCTGCCCTGGGCTTCGGGGCCAAGCTGCCCCCCGACGGCAGGGTGTCCCATGA; GTTTCCCCTGAATGGCAACCAGGAGAACCCCTCCTGCTGTGGCATCGATGGCATCCTGGAGGCCTACCAC; AGCAGCCTGCGCACCGTGCAGCTTTACGGCCCCACTAACTTTGCTCCTGTTGTCACTCACGTAGCCAGGA; ACGCCGCAGCTGTGCAGGATGGCTCCCAGTATTCTGTGCTGCTCATCATCACTGATGGTGTCATCTCGGA; CATGGCACAGACCAAGGAGGCAATTGTAAATGCTGCCAAACTCCCTATGTCGATCATCATTGTTGGCGTG; GGCCAGGCCGAGTTCGATGCCATGGTGGAGCTGGACGGTGATGACGTGCGGATCTCCTCCAGGGGGAAGC; TGGCTGAGCGAGACATTGTCCAGTTTGTGCCCTTCAGGGACTATGTGGACCGCACCGGCAACCACGTACT; GAGCATGGCCCGCCTGGCCCGGGATGTTCTGGCTGAGATTCCGGACCAACTGGTGTCCTACATGAAGGCA; CAGGGCATCCGCCCACGCCCGCCACCTGCTGCACCCGCTCAGTCACCCCCACAGTCCCCAGCCCACTCAC; CTCCTGGATCCCCCGTGCACACGCATATCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153166
• Insert Size: 1782 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153166.2, NP_694806.1
• RefSeq Size: 4366 bp
• RefSeq ORF: 1782 bp
• Locus ID: 240058
• UniProt ID: Q8JZW4
• Cytogenetics: 17 A3.3
• Summary: Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene is one of several genes that encodes a calcium-dependent protein containing two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. It encodes isoform 2 which is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.