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Eefsec (NM_023060) Mouse Untagged Clone

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SKU
MC219303
Eefsec (untagged) - Mouse eukaryotic elongation factor, selenocysteine-tRNA-specific (Eefsec), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $598.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Eefsec
Synonyms mSelB; sec; Selb
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC219303 representing NM_023060
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGGCCGGCGGGTAAATGTGAATGTGGGCGTGCTGGGTCACATCGACAGTGGCAAAACAGCGCTGG
CGCGCGCGCTGAGCACCACGGCCTCCACTGCTGCCTTCGACAAGCAGCCGCAGAGCCGCGAGCGCGGCAT
AACGCTCGACCTGGGCTTCTCCTGTTTTGTCGTGCCGCTGCCGGGGGCAGAACCCGGTTCTAGCGACACG
CTGCTGCAGGTCACACTGGTCGACTGCCCCGGGCACGCCTCCCTCATCCGGACCATCATTGGCGGTGCCC
AGATCATTGATTTGATGATGCTCGTGATTGATGTGACCAAGGGAATGCAGACACAGTCCGCAGAGTGCCT
TGTGATTGGCCAGATCGCCTGCCAGAAGCTGGTTGTGGTGCTGAACAAAATAGACCTGTTAGCAGAAGGG
AAGAGACAGGCAGCGATTGACAAAATGACCAAGAAAATGCAGAAGACTCTTGAGAACACCAAATTCCGGG
GTGCACCGATTATTCCCGTGGCAGCCAAACCTGGGGGACCAGAAGCCCCTGAAACTGAAGCTCCACAGGG
CATCTCAGAACTCATTGAGCTCCTGAAGTCCCAGATTTCCATCCCAACGAGAGACCCCTCTGGACCATTC
CTTATGTCTGTGGACCACTGCTTCTCCATCAAGGGTCAAGGCACCGTGATGACTGGGACTATCCTCTCAG
GCACTATCAGCCTTGGGGACAGTGTGGAGATCCCTGCCCTCAAGGTGGTGAAGAAAGTGAAGTCCATGCA
GATGTTCCATACACCCGTCACCTCTGCCATGCAGGGGGACCGACTGGGCATCTGTGTCACCCAGTTTGAC
CCCAAGCTGTTGGAGCGTGGGCTGGTATGTGCACCCGAGTCTCTGCACACTGTTCACGCAGCCCTCATCT
CTGTGGAGAAGATCCCTTACTTCCGAGGACCCCTGCAGACCAAGGCCAAGTTCCATATCACCGTTGGCCA
TGAGACAGTCATGGGTCGCACGCTGTTCTTCAGCCCTGCTCCTGACAGCTTTGACTTGGAGCCCGTGCTA
GATTCCTTCGACCTTTCCCGAGAGTACCTCTTCCAAGAGCAGTACCTGTGCAAGGATTCCATGCCCACAG
CCACAGAAGGTGATGACGAGGCGGACCCCAAAGCCGGCCACGCCCCAGGGGGCCACTGCCCCCGGCAGCA
GTGGGCCCTGGTAGAGTTTGAGAAGCCAGTCACCTGCCCCCGACTCTGCTTGGTGATTGGCTCCAGACTA
GATGCCGACATTCACACCAACACATGCCGGCTGGCCTTCCATGGTGTCTTGCTGCAAGGGCTGGAGGACA
AGAACTACATTGAGAGCTTCCTGCCAGCACTGAGGGTTTACAAGCTGAAGCACAAGCATGGCCTTGTGGA
GCGGGTAATGGATGACTACAGCGTGATTGGACGTTCCCTGTTCAAAAAGGAGACCAACATCCAGCTCTTT
GTGGGGCTTAAGGTGCAGCTGTCCACAGGGGAACAGGGTATCATCGACAGTGCCTTCGGCCAAAGTGGCA
AGTTCAAGATCCACATCCCTGGTGGCCTCAGCCCTGAGTCCAAGAAGATCCTGACACCTACACTCAAGAA
GCGGAGCCGAGCCGGCCGTGGAGAGACCACCAAGCCAGAGGAGGGCACTGAACGGCCAGAGCCCATCCAG
CCTGTGACACTCAACCTGTCTTTCAAGCGCTATGTCTTTGACACCCAGAAGCGCATGGTACAGACTCCCT
GA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_023060
Insert Size 1752 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023060.3, NP_075547.1
RefSeq Size 2679 bp
RefSeq ORF 1752 bp
Locus ID 65967
UniProt ID Q9JHW4
Cytogenetics 6 D1
Summary Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.UniProtKB/Swiss-Prot Function
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"NM_023060" in other vectors (4)
SKU Description Size Price
MG216743 Eefsec (tGFP-tagged) - Mouse eukaryotic elongation factor selenocysteine-tRNA-specific (Eefsec), (10ug) 10 ug
$743.00
MR216743 Eefsec (Myc-DDK-tagged) - Mouse eukaryotic elongation factor, selenocysteine-tRNA-specific (Eefsec) 10 ug
$289.00 $543.00
MR216743L3 Lenti ORF clone of Eefsec (Myc-DDK-tagged) - Mouse eukaryotic elongation factor, selenocysteine-tRNA-specific (Eefsec) 10 ug
$843.00
MR216743L4 Lenti ORF clone of Eefsec (mGFP-tagged) - Mouse eukaryotic elongation factor, selenocysteine-tRNA-specific (Eefsec) 10 ug
$843.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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