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Ehd2 (NM_153068) Mouse Untagged Clone

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SKU
MC218658
Ehd2 (untagged) - Mouse EH-domain containing 2 (Ehd2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $557.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ehd2
Synonyms BC027084; C130052H20Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC218658 representing NM_153068
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCAGCTGGCTGAAGAAGGGCGGGGCCCGGGGCCAGCGACCTGAGGCCATCCGCACCGTGACCTCAT
CCCTCAAGGAGCTGTATCGCACGAAACTTCTTCCCCTGGAGGAACACTACCGTTTTGGGTCTTTCCATTC
ACCGGCCCTTGAAGATGCTGACTTCGACGGCAAACCCATGGTGCTGGTGGCCGGCCAGTATAGCACCGGC
AAAACCAGCTTCATCCAGTACCTTCTGGAGCAGGAAGTCCCTGGCTCCCGCGTGGGTCCCGAGCCCACCA
CCGACTGCTTCGTGGCTGTCATGCATGGCGAGACAGAGGGCACCGTGCCCGGAAATGCTCTCGTTGTGGA
CCCAGAGAAGCCCTTCCGCAAACTCAACCCTTTTGGAAACACTTTTCTCAACAGGTTTATGTGCGCCCAA
CTCCCCAACCAGGTCCTGGAGAGCATCAGCATCATCGACACGCCTGGCATCCTATCAGGTGCAAAACAGA
GAGTGAGCCGTGGCTACGACTTCCCAGCTGTCCTGCGCTGGTTTGCTGAACGCGTGGACCTCATCATCTT
GCTCTTTGATGCCCACAAGCTGGAGATTTCCGATGAATTCTCGGAGGCCATCGGTGCCTTGCGTGGCCAC
GAGGATAAGATCCGCGTGGTACTCAACAAGGCAGACATGGTGGAGACACAGCAGCTGATGCGCGTCTACG
GGGCGCTCATGTGGGCGCTGGGCAAGGTGGTGGGCACGCCTGAGGTCCTTCGCGTCTACATTGGCTCCTT
CTGGTCCCAGCCGCTCCTTGTGCCGGACAACCGGCGTCTTTTCGAGCTGGAGGAACAGGACCTCTTCCGT
GATATCCAAGGCCTACCGCGCCATGCGGCGCTGCGCAAGCTCAACGACCTAGTGAAACGGGCGCGGCTGG
TGCGGGTACACGCTTACATCATCAGCTACCTGAAGAAAGAGATGCCCACGGTGTTCGGGAAGGAAAACAA
GAAGAAGCAGCTGATCCTGAAGCTGCCTGTCATCTTTGCGAAGATCCAGCTGGAGCACCACATCTCCCCT
GGAGACTTTCCTGACTGCCAGAAGATGCAGGAGCTGTTGATGGCACATGACTTCACCAAGTTCCACTCGC
TAAAGCCGAAGCTGCTGGAGGCGCTAGATGACATGCTGGCCCAAGACATTGCCAAGCTTATGCCGCTGCT
TCGGCAGGAGGAGCTGGAGAGTGTCGAGGCTGGAGTGCAGGGCGGTGCGTTTGAGGGCACCCGCATGGGC
CCCTTCGTGGAGCGGGGCCCTGATGAAGCAATAGAGGATGGTGAGGAGGGCTCAGAAGATGATGCCGAAT
GGGTGGTGACCAAGGACAAGTCCAAATACGATGAAATCTTCTACAACTTAGCTCCTGCTGATGGCAAGCT
GAGTGGCTCTAAGGCCAAAACCTGGATGGTGGGCACAAAGCTACCCAACTCTGTGCTGGGGAGAATCTGG
AAGCTGAGCGATGTCGACCGAGACGGCATGCTGGATGATGAGGAGTTTGCCTTGGCCAGCCACCTCATTG
AGGCTAAGCTCGAAGGCCATGGTCTACCCACCAACCTACCCCGCCGCCTGGTGCCGCCTTCTAAGCGACG
ACAGAAGGGCTCTGCTGAATGA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_153068
Insert Size 1632 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153068.3, NP_694708.2
RefSeq Size 3058 bp
RefSeq ORF 1632 bp
Locus ID 259300
UniProt ID Q8BH64
Cytogenetics 7 8.65 cM
Summary ATP- and membrane-binding protein that controls membrane reorganization/tubulation upon ATP hydrolysis (PubMed:24508342). Plays a role in membrane trafficking between the plasma membrane and endosomes. Important for the internalization of GLUT4 (PubMed:14676205). Required for fusion of myoblasts to skeletal muscle myotubes. Required for normal translocation of FER1L5 to the plasma membrane (PubMed:18502764, PubMed:21177873). Regulates the equilibrium between cell surface-associated and cell surface-dissociated caveolae by constraining caveolae at the cell membrane (By similarity).UniProtKB/Swiss-Prot Function
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"NM_153068" in other vectors (4)
SKU Description Size Price
MG220542 Ehd2 (tGFP-tagged) - Mouse EH-domain containing 2 (Ehd2), (10ug) 10 ug
$706.00
MR220542 Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (Ehd2) 10 ug
$289.00 $506.00
MR220542L3 Lenti ORF clone of Ehd2 (Myc-DDK-tagged) - Mouse EH-domain containing 2 (Ehd2) 10 ug
$806.00
MR220542L4 Lenti ORF clone of Ehd2 (mGFP-tagged) - Mouse EH-domain containing 2 (Ehd2) 10 ug
$806.00

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