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D10Ertd610e (BC054833) Mouse Untagged Clone

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SKU
MC218309
D10Ertd610e (untagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol D10Ertd610e
Synonyms 2410008H17Rik; D10Ertd610e; GEFT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC054833
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTATGTGGTGTACTGCCAGAATAAACCCAAGTCAGAGCATGTGCTGTCAGAGTTTGGGGACAGCTACT
TTGAGGAGCTGAGGCAGCAGCTGGGACACCGACTGCAGCTCAATGACCTCCTCATTAAACCAGTGCAGCG
AATCATGAAATACCAGTTGCTGCTGAAGGATTTTCTAAAGTATTACAGAAGAGCTGGGAAGGATACTGAA
GAGCTGGAGCAAGCTGTGGAGGTCATGTGTTTCGTACCTAAGCGATGCAATGATATGATGTCCCTGGGGA
GACTTCGGGGATTTGAGGGAAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACATTCTTGGTTAC
AGAGCCTGAAGCTGGGGGTCTGCTTTCTTCCCGGGGTCGGGAGAGGCGTGTCTTCCTGTTCGAGCAAATC
ATCATCTTCAGTGAGGCCCTGGGAGGAGGAGGCCGAGGTGGCGCGCAGCCTGGCTACGTGTACAAGAACA
GCATCAAGGTGAGCTGCCTAGGGCTGGAGGGGAATCTCCAAGGCAACCCTTGCCGGTTTGCGCTGACCTC
TAGAGGGCCAGAAGGTGGGATCCAGCGCTACGTTCTGCAGGCTTCAGACCCTGCTGTCAGTCAAGCCTGG
ATAAAGCAAGTGGCCCAGATCCTGGAAAGCCAGCGTGACTTTCTCAACGGTGAAGCTCCCATCCTCTCCC
TTCAGAGGCTCCCAAAGCTATCTGACCTCCTGAACCCCTTCCCCAGCCACGCAGTCTCCAACCCTTGCTT
CTTTGAGCCTAACTTCTCTCCATCCTACACCCACCCGAAACCCTCTAAAGTTCCAAGCACCTTACATTGG
CCAAATGAGGAAGAAGGTGAGGCAGGGAAAGTGATCGGTCTCTTCTGTAATACATCTCTGTTCCTTCCCT
TGGCCCAGCATTACAGTCACCCATTGAATACCAGAGACGTGAGAGCCAGACCAATAGCCTGGGGCGACCA
GGAGGGCCTTGGGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC054833
Insert Size 996 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC054833, AAH54833
RefSeq Size 1732 bp
RefSeq ORF 995 bp
Locus ID 52666
Cytogenetics 10 74.5 cM
Summary May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation (By similarity). May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.UniProtKB/Swiss-Prot Function
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"BC054833" in other vectors (4)
SKU Description Size Price
MG204889 D10Ertd610e (tGFP-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) 10 ug
$500.00
MR204889 D10Ertd610e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) 10 ug
$289.00 $300.00
MR204889L3 Lenti ORF clone of D10Ertd610e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) 10 ug
$600.00
MR204889L4 Lenti ORF clone of D10Ertd610e (mGFP-tagged) - Mouse DNA segment, Chr 10, ERATO Doi 610, expressed (cDNA clone MGC:62781 IMAGE:6439728) 10 ug
$600.00

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