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Smo (BC048091) Mouse Untagged Clone

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SKU
MC218049
Smo (untagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Smo
Synonyms D13Mgi8, Smoh
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC048091
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTGGCCGCCCCGTGCGTGGGCCCGAGCTGGCGCCCCGGAGGCTGCTGCAGTTGCTGCTGCTGG
TACTGCTGGGGGGCCCGGGCCGGGGGGCGGCCTTGAGCGGGAACGTGACCGGGCCTGGGCCTCACAGCGC
CAGCGGGAGCTCGAGGAGGAACGTGCCGGTGACCAGCCCTCCGCCGCCGCTGCTGAGCCACTGCGGCCGG
GCCGCCCACTGCGAGCCTTTGCGCTACAACGTGTGCCTGGGCTCGGCGCTGCCCTACGGAGCCACCACCA
CGCTGCTGGCTGGGGACTCGGACTCGCAGGAGGAAGCGCACGGCAAGCTCGTGCTCTGGTCCGGCCTCCG
GAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGTGCTGTCTACATGCCCAAGTGTGAGAAT
GACCGAGTGGAGTTGCCCAGCCGTACCCTCTGCCAGGCCACCCGAGGCCCCTGTGCCATTGTGGAGCGGG
AGCGAGGGTGGCCTGACTTTCTGCGTTGCACACCGGACCACTTCCCTGAAGGCTGCCCAAACGAGGTACA
AAACATCAAGTTCAACAGCTCAGGCCAATGTGAAGCACCCTTGGTGCGAACAGACAACCCCAAGAGCTGG
TATGAGGACGTGGAGGGCTGTGGGATTCAGTGTCAGAACCCGCTGTTCACCGAGGCCGAGCACCAGGACA
TGCACAGCTACATCGCAGCCTTCGGGGCGGTCACTGGTCTCTGCACGCTCTTCACACTGGCCACCTTTGT
GGCTGACTGGCGGAACTCCAATCGCTACCCTGCGGTTATTCTCTTCTATGTCAATGCGTGTTTCTTCGTG
GGCAGCATTGGCTGGCTGGCCCAGTTCATGGATGGTGCCCGCCGAGAGATTGTTTGCCGAGCAGATGGCA
CCATGAGATTTGGGGAGCCCACCTCCAGTGAGACCCTGTCCTGTGTCATCATCTTTGTCATTGTGTACTA
TGCCTTGATGGCTGGAGTAGTCTGGTTCGTGGTCCTCACCTATGCCTGGCACACCTCCTTCAAAGCCCTG
GGCACCACCTACCAGCCTCTCTCGGGCAAGACATCCTATTTCCACCTGCTCACGTGGTCACTCCCCTTTG
TCCTCACGGTGGCAATCCTGGCTGTGGCTCAGGTAGATGGAGACTCCGTGAGTGGCATCTGTTTTGTAGG
CTACAAGAACTATCGGTACCGTGCTGGCTTTGTCCTGGCCCCAATTGGCCTGGTGCTTATTGTGGGAGGC
TACTTCCTCATCACAGGGGTCATGACTCTGTTCTCCATCAAGAGCAACCACCCCTGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC048091
Insert Size 1320 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC048091, AAH48091
RefSeq Size 1747 bp
RefSeq ORF 1319 bp
Locus ID 319757
Cytogenetics 6 12.36 cM
Summary G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO) (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).UniProtKB/Swiss-Prot Function
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"BC048091" in other vectors (4)
SKU Description Size Price
MG206991 Smo (tGFP-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) 10 ug
$657.00
MR206991 Smo (Myc-DDK-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) 10 ug
$289.00 $457.00
MR206991L3 Lenti ORF clone of Smo (Myc-DDK-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) 10 ug
$757.00
MR206991L4 Lenti ORF clone of Smo (mGFP-tagged) - Mouse smoothened homolog (Drosophila) (cDNA clone MGC:57078 IMAGE:6486595) 10 ug
$757.00

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