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Mrps27 (BC049149) Mouse Untagged Clone

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SKU
MC217989
Mrps27 (untagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mrps27
Synonyms 2610028H14Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC049149
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGCAAGAAGCCTTTGACGTGCCGTCCACCCAGTTTCTCTCCCTCTATGTTTTATACCGTTGCCTAG
CAGAGAAGACGGAGCTCACATGGGAAGAAGAAAGAGACTTTGGGGCGTCCCTCTTGCTTTCTGGCCTGAA
ACAAAGGAACACAGTGGGCCTGAGTTCCCAGCTGTATGGCTATGCACTTCTCGGGAAGGTGGAACTGCAG
CGTGGGGTGCGGGCTGTATACCACGGCATGCCTCTGATGTGGACACCAGGCTACCTCGACAGAGCCCTCC
AAGTGATGGAGAGGGTGGCCTCCTCCCCTGAAGACCTCAAGCTGGGTAGAGAAGTGCTCGATGTGCTGGA
TGGAGTCCTGAAGGTGGTGACGTCTCCAGATGTCCAGACTTCAGAGGCACAGCCTCAGGAAGGTGAAGAT
GGCTTGGGCTCAGCAAACCTGGTGGAGCAGCTAGACACTGAGGAACCAGAACAGTCCAAGCTTCCCCGAT
ACCTCGAGCGCTTTCAGGCCTCTCGGTCTAAACTGCAGGAGCTGAACAGAGTTGAGTCTGAAAGTCTTTT
GACTCTGACCACCCAGCTTGTAAAAGAAAAACTCCCCGCCTGTGAGGCCGAGGACCTGGCCACCTACGAG
CAGAAACTCCGTGAGTGGCACCTGGAGCGGGTGCAGTTGATCCAGAGGGAGCAGGAACAGAGGGAGAAGG
CCAAGCAAGAGTACCAGGCGCTGAGTGCGGCAGAGAAAGCAGCCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC049149
Insert Size 747 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC049149, AAH49149
RefSeq Size 1833 bp
RefSeq ORF 746 bp
Locus ID 218506
Cytogenetics 13 D1
Summary RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues.UniProtKB/Swiss-Prot Function
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"BC049149" in other vectors (4)
SKU Description Size Price
MG203136 Mrps27 (tGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) 10 ug
$500.00
MR203136 Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) 10 ug
$289.00 $300.00
MR203136L3 Lenti ORF clone of Mrps27 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) 10 ug
$600.00
MR203136L4 Lenti ORF clone of Mrps27 (mGFP-tagged) - Mouse mitochondrial ribosomal protein S27 (cDNA clone MGC:56872 IMAGE:6309524) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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