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Trim23 (BC059017) Mouse Untagged Clone

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SKU
MC217929
Trim23 (untagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $589.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trim23
Synonyms 6330516O20Rik; AI450195; Arfd1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC059017
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCCCTCGCTGTAAACAAGCCCGGAGCCGGAGTAGACAGTGGGCGGCAGGGCAGCCGGGGGACGG
CTGTGGTGAAGGTTCTGGAGTGTGGGGTTTGTGAAGATGTCTTCTCTTTGCAAGGAGATAAAGTTCCTCG
CCTCCTCCTCTGTGGCCACACAGTCTGTCATGACTGTCTTACTCGCTTGCCTCTGCATGGAAGAGCAATA
CGTTGCCCATTTGATCGACAAGTAACAGACCTAGGAGACTCAGGTGTATGGGGTTTGAAAAAGAACTTTG
CTTTACTGGAGCTTTTAGAACGTTTGCAAAATGGGCACATTGGTCAGTATGGAGCTGCAGAAGAGGCCAT
TGGGACATCTGGAGAGAGCATCATTCGCTGTGATGAAGATGAAGCTCACGTTGCATCTGTATATTGCACT
GTGTGTGCAACTCACTTGTGCTCAGACTGTTCTCAAGTTACTCATTCTACAAAGACATTAGCAAAGCATA
GGCGTGTGCCTCTAGCTGATAAACCTCATGAGAAAACCATGTGCTGTCAGCACCAGGTGCATGCCATTGA
GTTTGTTTGCTTGGAAGAAGGTTGTCAAACTAGTCCACTCATGTGCTGTGTCTGCAAAGAATATGGAAAG
CACCAAGGTCACAAGCATTCAGTACTGGAACCAGAAGCTAATCAGATCCGAGCATCAATTTTAGACATGG
CTCACTGCATACGGACCTTCACTGAGGAAATCTCAGATTATTCCAGAAAATTAGTTGGGATTGTTCAGCA
CATTGAAGGAGGAGAACAAATAGTAGAAGATGGAATTGGAATGGCTCACACAGAACATGTCCCAGGTACT
GCAGAGAATGCCCGATCATGTGTCAGAGCTTATTTTTCTGATTTACATGAAACTCTTTGTCGTCAAGAAG
AAATGGCTCTAAGTGTTGTTGATGCCCATGTTCGAGAAAAACTGATTTGGCTTAGGCAGCAACAAGAGGA
TATGACTATTCTCTTGTCCCAGGTCTCAACAGCCTGTCTCCACTGTGAAAAAACTCTGCAGCAGGATGAT
TGCAGAGTTGTCTTGGCAAAACAAGAAATCACAAGATTACTAGAAACACTGCAGAAACAGCAGCAGCAGT
TTACAGAGGTTGCAGATCATATTCAGTTGGATGCCAGTATTCCAGTCACTTTTACAAAGGACAATAGAGT
TCATATTGGACCCAAAATGGAAATCCGAGTAGTCACATTAGGATTAGATGGTGCTGGAAAAACTACCATT
TTGTTCAAGTTAAAACAAGATGAATTTATGCAACCTATTCCAACAATTGGTTTTAATGTGGAAACTGTGG
AATACAAAAATCTAAAATTCACCATTTGGGATGTGGGTGGAAAACACAAATTAAGACCATTGTGGAAACA
TTATTACCTCAATACACAAGCTGTTGTATTTGTTGTTGATAGTAGTCATAGAGACAGAATTAGTGAAGCA
CACAGTGAACTTGCAAAGTTGTTAACGGAAAAAGAACTCCGAGATGCCTTACTCTTGATTTTTGCTAACA
AACAGGATGTAGCTGGAGCACTTTCAGTAGAAGAAATCACTGAACTTCTCAGTCTCCATAAACTATGCTG
TGGAAGGAGTTGGTATATTCAGGGCTGTGATGCTCGAAGTGGAATGGGGCTCTACGAAGGGTTGGACTGG
CTGTCCCGGCAACTTGTGGCTGCTGGAGTGTTGGATGTTGCTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC059017
Insert Size 1725 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC059017, AAH59017
RefSeq Size 3385 bp
RefSeq ORF 1724 bp
Locus ID 81003
Cytogenetics 13 D1
Summary Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.UniProtKB/Swiss-Prot Function
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"BC059017" in other vectors (4)
SKU Description Size Price
MG209027 Trim23 (tGFP-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) 10 ug
$734.00
MR209027 Trim23 (Myc-DDK-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) 10 ug
$289.00 $534.00
MR209027L3 Lenti ORF clone of Trim23 (Myc-DDK-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) 10 ug
$834.00
MR209027L4 Lenti ORF clone of Trim23 (mGFP-tagged) - Mouse tripartite motif protein 23 (cDNA clone MGC:69677 IMAGE:6848690) 10 ug
$834.00

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