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Wdfy1 (BC025226) Mouse Untagged Clone

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SKU
MC217861
Wdfy1 (untagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Wdfy1
Synonyms 1700120F24Rik, WDF1, FENS-1, Jr1, ZFYVE17
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC025226
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTTTATCAAGACCTATCCAGCCCACCAGAACCGCGTGTCTGCTATCATCTTCAGCCTGTCTGCCG
AGTGGGTGATCAGCACTGGCCACGACAAGTGTGTGAGCTGGATGTGCACGCGCAGCGGGAATATGCTTGG
CCGCCACTTCTTCTCGTCGTGGGCTTCCTGTTTGCAGTATGATCTGGACACTCAGCACGCCTTTGTTGGC
GATTACTCGGGACAGATCACCCTGCTGAAGCTGGAGCAGAACACTTGTTCAGTTATCACGACCCTCAAGG
GACATGAAGGTAGCATCGCCTGCCTTTGGTGGGACCCTATCCAACGGTTACTCTTCTCGGGAGCATCCGA
CAACAGCGTCATCATGTGGGACATCGGGGGACGGAAAGGCCGGACACTCCTCCTTCAAGGCCACCATGAC
AGAGTGCAGTCACTGTGCTACCTGCAGCTCACGAGGCAGCTCGTCTCCTGCTCGGCAGATGGTGGCATTG
CAGTGTGGAACATGGATGTTAGCAGAGAAGAGGCTCCTCAGTGGTTAGAAAGCGACTCCTGTCAGAAGTG
TGAGCAGCCCTTCTTCTGGAATATAAAACAGATGTGGGACACAAAGACCCTGGGCTTGAGGCAGCATCAC
TGCAGGAAGTGTGGGCAGGCCGTCTGTGGGAAGTGCAGCAGCAAGCGGTCCAGTTACCCCGTCATGGGTT
TTGAGTTCCAGGTTCGAGTTTGTGATTCCTGTTACGACTCCATCAAAGATGAGGACCGGACTTCTCTAGC
AACATTTCATGAAGGAAAACATAACATTTCCCATATGTCCATGGACATTGCAAGGGGACTGATGGTGACC
TGTGGGACAGATCGTGTTGTGAAGATATGGGATATGACTCCTGTGGTGGGCTGCAGCCTGGCAACTGGAT
TTTCTCCACACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC025226
Insert Size 924 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC025226, AAH25226
RefSeq Size 1363 bp
RefSeq ORF 923 bp
Locus ID 69368
Cytogenetics 1 C4
Summary Positively regulates TLR3- and TLR4-mediated signaling pathways by bridging the interaction between TLR3 or TLR4 and TICAM1. Promotes TLR3/4 ligand-induced activation of transcription factors IRF3 and NF-kappa-B, as well as the production of IFN-beta and inflammatory cytokines (PubMed:25736436).UniProtKB/Swiss-Prot Function
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"BC025226" in other vectors (4)
SKU Description Size Price
MG204332 Wdfy1 (tGFP-tagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404) 10 ug
$500.00
MR204332 Wdfy1 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404) 10 ug
$289.00 $300.00
MR204332L3 Lenti ORF clone of Wdfy1 (Myc-DDK-tagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404) 10 ug
$600.00
MR204332L4 Lenti ORF clone of Wdfy1 (mGFP-tagged) - Mouse WD repeat and FYVE domain containing 1 (cDNA clone MGC:32262 IMAGE:5011404) 10 ug
$600.00

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