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Cgref1 (NM_001160149) Mouse Untagged Clone

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SKU
MC217855
Cgref1 (untagged) - Mouse cell growth regulator with EF hand domain 1 (Cgref1), transcript variant 2, (10ug)
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ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $330.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Cgref1
Synonyms 1110004G24Rik; AW045201; Cgr11
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217855 representing NM_001160149
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCAGTGGCTGATGCAAGCGTTGATGCTGCCACTATTGCTGCTCCCTTTAGGTCGAGCTGCTCCCA
AGGATGGAGTTGCAAGGTTGGACCCTGAGGTACAACAGCAGCTCACACCCAACCCTTTCCAGCCAGGCCC
TGAGCAGCTCCGACATCTGCAGAATTATCTCAAGGGACTAGAGAAGATGGAAGAGGATCCTGAGCACATG
GACCGGGAGCAAGTCCTGCTTTCGCTCTTTGCTCTTCATGACTATGACCAGAATGGACAGCTGGACGGCC
TGGAGCTACTGTCCATGCTGACAGCAGCTCTGGCCCCTGGAGCTGCACACTTTCCCATCAACCCGGTGAT
CCTAGTAGTAGACTCGGTGCTCGAGACTCAGGACCTGGATGGAGACGGGCTCATGACTCCTGCAGAGCTC
ATCAACTTCCCAGAAGTCCCCAAACACACAGAGTCCCTTCCCCCAGCTCTCCAGGAGCCACAACCTGCTG
GAAGTCAGCCACTTTTAGCCAACAGTCCACTGCAGTCAGAAACCCAGCAGTCCCTGGGGACTAAAGAAAT
TAGGAGCCAGGTAGAGGCCAAGAGGGCGTCCTTGGAGCCTGAACAGGAGGCTGGACATCAGACAGAGGGA
AAAGTAGATACCCTAAGCCCTGAAGAGGAGGCTAGGGGACAGGCAGAGTCTGAAGGAGATGTTCCAGGTC
CCAGAGAAGGTGCTGAGGAACAGGTGGAGATCAAGGACAATGAAGGAGAAGCCAAAGAACTGCTGGTGGA
AACACTGGAGAGCCTAAACACTCCAAATGAGGCTGAGGCTCATAGCATCCAATTGGAGAACGATGAGATA
TGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001160149
Insert Size 843 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001160149.1, NP_001153621.1
RefSeq Size 1266 bp
RefSeq ORF 843 bp
Locus ID 68567
UniProt ID Q8R1U2
Cytogenetics 5 B1
Summary Mediates cell-cell adhesion in a calcium-dependent manner. Able to inhibit growth in several cell lines (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream translational start codon, compared to variant 1. The resulting isoform (2) is shorter at the N-terminus, compared to isoform 1.
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Documents
Resources
"NM_001160149" in other vectors (3)
SKU Description Size Price
MR203802 Cgref1 (Myc-DDK-tagged) - Mouse cell growth regulator with EF hand domain 1 (Cgref1), transcript variant 2 10 ug
$289.00 $300.00
MR203802L3 Lenti ORF clone of Cgref1 (Myc-DDK-tagged) - Mouse cell growth regulator with EF hand domain 1 (Cgref1), transcript variant 2 10 ug
$600.00
MR203802L4 Lenti ORF clone of Cgref1 (mGFP-tagged) - Mouse cell growth regulator with EF hand domain 1 (Cgref1), transcript variant 2 10 ug
$600.00

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