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Net1 (NM_001047159) Mouse Untagged Clone

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SKU
MC217799
Net1 (untagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $555.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Net1
Synonyms mNET1; Net1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217799 representing NM_001047159
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCGCATGATGAGATCGGAGGTCTCCTGCCCATTAAAAGGACGATCCGAGTCTTAGATGTTAATA
ACCAGCCCTTCCGGGAGCAGGAGGAGCCAAGCAATAAGAGAGTTCGGCCTCTAGCTCGGGTCACATCTTT
GGCAAACTTAATCTCTCCCGTAAGAAATGGAGCAGTCAGGCGCTTCGGTCAGACCATACAGTCGTTTACC
CTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTCATTCAGCAGGTCCACCGTCCCCACACCCA
CCAAGAGAAGAAGCAGTGCACTATGGTCGGAAATGCTAGACATCAATATGAAGGAGTCCTTGACCACTCG
AGAGATCAAACGTCAGGAGGCCATATATGAATTGTCCCGAGGGGAACAGGACTTGATTGAGGATCTCAAG
CTCGCTAGAAAGGCCTACCATGACCCCATGTTAAAGCTGTCTATCATGTCAGAAGAGGAACTCACGCACA
TATTTGGAGACTTGGATGCTTATATACCTCTGCACGAAGATCTGTTAGCAAGAATTGGAGAAGCAACCAA
GCCTGATGGGACTGTGGAGCAGATCGGTCACATTCTCGTGAACTGGTTACCAGGCCTGAATGCCTACAGA
GGCTACTGCAGTAACCAGCTGGCGGCCAAGGCTCTTCTGGATCAGAAGAAACAAGACCCAAGAGTCCAAG
ACTTCCTCCAGCGCTGTCTGGAGTCTCCTTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCC
TCGAAGTCGCCTCGTCAAGTACCCCTTACTGTTAAAAGAAATCCTTAGACACACTCCCAAAGACCACCGT
GATGTTCAGCTTCTAGAAGAGGCAATATTGATAATACAAGGAGTTCTTTCTGATATCAACTTGAAGAAAG
GCGAATCTGAGTGTCAGTATTACATCAACAAGCTGGAGTACCTGGACGAGAAGCAGAAGGACCCTAGGAT
CGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGAAGAATAAAAGCGGCCATAAACTGTACATTTTC
CTATTTCAAGACATCTTGGTTTTGACTCGGCCTGTCACCCGAAATGAGCGCCACTTGTACCAGGTTTACC
GGCAGCCCATCCCAGTCCAGGAGCTGGTGCTGGAAGACCTGCAGGACGGGGATGTGCGCATGGGTGGCTC
CTTCCGAGGGGCCTTCGGCAACTCAGACAAAGCTAAAAATATCTTTAGGGTCCGCTTCCAAGACCCTTCT
CCAGGCCACTCCCACACACTGCAAGCCAATGATGTGTTCCATAAGCAGCAGTGGTTCAACTGTATTCGCG
CGGCCATTGCCCCCTTCCAGCGGGCCGCCAGCCCCTTAGAGCTTCAGGGCTTGCCAGACCTCCACGAAGA
GTGTGAAGAGAACAACCCTTCTGCTGGGAACCTCAGAGCCCAGCGACGGTCATGCGTGGTTCCTGGTGTC
ATGCAGATAGACGAGGAAAGCGCCCTGGATTGTGGCTCCAGTGTACAGACAGTGGAAGACACCAGGAACA
TGAAGGCACAGCGGCCTCAGCCTGGCTTACGAAGAGCAAGGGACAAAGCACAGTCAGGTGGCAAAAAGAA
AGAGACTTTGGTGTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001047159
Insert Size 1626 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001047159.2, NP_001040624.1
RefSeq Size 3874 bp
RefSeq ORF 1626 bp
Locus ID 56349
UniProt ID Q9Z206
Cytogenetics 13 A1
Summary Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway. Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (2) lacks part of the 5' coding region, uses an alternate 5'-terminal exon, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.
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"NM_001047159" in other vectors (3)
SKU Description Size Price
MR208638 Net1 (Myc-DDK-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2 10 ug
$289.00 $505.00
MR208638L3 Lenti ORF clone of Net1 (Myc-DDK-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2 10 ug
$805.00
MR208638L4 Lenti ORF clone of Net1 (mGFP-tagged) - Mouse neuroepithelial cell transforming gene 1 (Net1), transcript variant 2 10 ug
$805.00

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