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Tead2 (BC050217) Mouse Untagged Clone

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SKU
MC217755
Tead2 (untagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Tead2
Synonyms ETF, TEF4, TEAD-2, TEF-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC050217
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGATCCCCGGACTGGGGCCCCCCTGGATGATGGCGGGGGCTGGACAGGTAGCGAGGAAGGCAGCG
AAGAGGGCACCGGAGGCAGCGAGGGCGTTGGAGGTGATGGCAGCCCTGATGCAGAAGGCCGGAACGAACT
CATTGCCCGTTACATCAAGCTGAGGACAGGGAAGACGAGAACGCGAAAGCAGGTCTCCAGCCATATTCAG
GTTTTGGCTCGAAGGAAATCGAGAGAAATTCAGTCCAAGCTGAAGGACCAAGTCTCCAAGGACAAGGCCT
TCCAGACGATGGCCACCATGTCTTCGGCACAGCTCATCTCCGCCCCTTCCCTCCAGGCCAAGCTGGGCCC
TTCTGGCCCTCAGGCCACTGAGCTTTTCCAGTTCTGGTCAGGGAGCTCTGGGCCACCATGGAATGTTCCA
GACGTGAAGCCCTTCTCACAGGCACCGTTCTCCGTGTCACTGACGCCCCCAGCCTCTGACCTACCAGGGT
ACGAGCCGCCCCCAGCCCTCTCACCCCTGCCCCCACCCGCTCCGTCTCCCCCAGCCTGGCAGGCTCGGGC
CCTGGGCACTGCCCGCCTGCAGCTGATAGAGTTCTCAGCGTTTGTGGAACCGCCAGACGCAGTTGACTCG
TTCCAGAGGCATCTGTTTGTCCACATCAGTCAGCAGTGTCCCAGCCCTGGAGCACCACCCCTAGAGAGTG
TGGACGTGCGGCAGATCTACGACAAATTCCCTGAGAAGAAGGGCGGCCTCCGCGAGCTGTATGACCGAGG
GCCACCACATGCCTTCTTCCTCGTCAAGTTCTGGGCGGACCTGAACTGGGGCCCCAGTGCCGAGGAGGCA
GGGAGCAGCGGAGGTGGCGGTGGCTTCTATGGAGTGAGCAGCCAGTATGAGAGCCGGGAGCTCATGACAC
TCACCTGCTCCTCCAAGGTCTGCTCCTTTGGCAAGCAAGTGGTAGAGAAGGTGGAGACGGAACGGGCCCA
GCTGGAGGACGGGCGCTTTGTGTACCGTCTGCTGCGCTCTCCCATGTGTGAGTACCTGGTTAATTTCCTG
CACAAGCTCCGTCAGCTGCCTGAACGCTACATGATGAACAGTGTCCTGGAGAACTTCACCATCCTCCAGG
TTGTGACAAACAGGGACACTCAGGAACTGCTGCTGTGTACTGCCTACGTCTTTGAAGTCTCCACCAGTGA
ACGAGGAGCCCAGTACCACATCTACCGCCTGGTCAGGGACTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC050217
Insert Size 1233 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC050217, AAH50217
RefSeq Size 1951 bp
RefSeq ORF 1232 bp
Locus ID 21677
Cytogenetics 7 29.19 cM
Summary Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction (By similarity). Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif.UniProtKB/Swiss-Prot Function
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"BC050217" in other vectors (4)
SKU Description Size Price
MG206438 Tead2 (tGFP-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393) 10 ug
$657.00
MR206438 Tead2 (Myc-DDK-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393) 10 ug
$289.00 $457.00
MR206438L3 Lenti ORF clone of Tead2 (Myc-DDK-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393) 10 ug
$757.00
MR206438L4 Lenti ORF clone of Tead2 (mGFP-tagged) - Mouse TEA domain family member 2 (cDNA clone MGC:60551 IMAGE:30021393) 10 ug
$757.00

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