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Sema5a (BC065137) Mouse Untagged Clone

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SKU
MC217745
Sema5a (untagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $524.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sema5a
Synonyms 5930434A13, semF
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217745 representing BC065137
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGGAGCCTGCATCCTTGCATGGCTGTTCTCAAGCCTGGGAGTGTGGAGACTTGCTAGGCCCGAGA
CCCAGGACCCTGCCAAGTGCCAGAGAGCTGAGCACCCTGTCGTCTCTTACAAAGAAATTGGCCCCTGGTT
ACGGGAGTTCAGAGCCGAGAATGCTGTGGATTTCTCGAGGTTAACATTTGACCCAGGACAGAAAGAACTT
GTCGTAGGAGCGAGAAACTATCTCTTCAGATTAGAGCTTGAGGATCTGTCTCTCATCCAGGCTGTGGAGT
GGGAGTGTGACGAAGCCACCAAGAAGGCCTGTTACAGCAAGGGCAAATCGAAGGAGGAATGTCAGAACTA
CATACGAGTGCTCTTAGTTGGTGGGGACCGGCTATTCACCTGTGGGACCAATGCCTTCACACCTGTCTGT
ACCATCCGCTCGTTAAGTAACCTGACTGAGATCCATGATCAGATCAGTGGCATGGCCCGCTGTCCCTACA
GTCCCCAGCACAATTCTACCGCCCTACTCACTGCCAGTGGTGAGCTCTATGCTGCAACAGCCATGGATTT
CCCAGGGCGAGATCCAGCCATTTACCGAAGTCTGGGCACTTTGCCTCCTCTTCGAACTGCACAGTACAAC
TCCAAATGGCTCAATGAACCAAACTTTGTGTCTTCCTACGACATCGGAAATTTCACCTACTTCTTCTTCC
GAGAAAACGCCGTGGAACATGACTGTGGGAAGACGGTGTTCTCCAGGGCTGCCCGGGTCTGTAAAAATGA
CATTGGAGGGCGATTTCTTCTGGAGGACACCTGGACTACCTTCATGAAGGCTCGCCTCAACTGCTCCCGG
CCTGGTGAGGTGCCCTTCTACTACAATGAGCTGCAGGGTACTTTCTTCCTGCCGGAGCTGGATCTGATCT
ATGGTATCTTCACCACCAATGTAAACAGCATCGCTGCCTCTGCTGTCTGTGTCTTCAACCTGAGCGCCAT
CTCACAGGCCTTCAATGGACCCTTCAAGTACCAAGAAAACTCTCGCTCAGCCTGGCTGCCTTATCCCAAC
CCCAACCCCAATTTTCAGTGCGGCACCATGGACCAGGGCCTGTACGTAAACCTGACGGAAAGAAACCTAC
AGGATGCTCAGAAGTTCATTCTGATGCATGAAGTGGTGCAGCCAGTGACCACAGTGCCCTCTTTCATGGA
GGACAACAGCCGCTTCTCCCACTTAGCTGTTGATGTTGTGCAAGGCAGGGAGACTCTGGTCCACATCATT
TATCTGGCCACAGATTATGGCACCATTAAGAAAGTACGAGCTCCCCTGAGTCAGAGCTCAGGCAGCTGTT
TGCTGGAAGAGATTGAGCTTTTCCCAGAGAGGAGGAGTGAGCCCATCAGGAGCCTGCAGATCCTCCACAG
CCAGAGTGTCCTATTTGTGGGACTACAGGAGCATGTGGCCAAGATCCCCCTGAAGAGGTGCCACTTCCAT
CAAACACGCAGTGCCTGCATTGGCGCTCAGGACCCTTACTGTGGCTGGGATGCGGTGATGAAGAAATGCA
CCAGCCTGGAGGAGAGTCTGAGCATGACCCAGTGGGATCAGAGCATCCCCACCTGTCCGACCAGAAATCT
CACTGTAGATGGGAGCTTTGGCCCATGGTCACCGTGGACACCCTGTACACACACTGATGGCATCTGGGCT
GGTGTGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI |
ACCN BC065137
Insert Size 1689 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC065137, AAH65137
RefSeq Size 4259 bp
RefSeq ORF 1688 bp
Locus ID 20356
Cytogenetics 15 13.02 cM
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Documents
Resources
"BC065137" in other vectors (4)
SKU Description Size Price
MG208910 Sema5a (tGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic 10 ug
$723.00
MR208910 Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic 10 ug
$289.00 $523.00
MR208910L3 Lenti ORF clone of Sema5a (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic 10 ug
$823.00
MR208910L4 Lenti ORF clone of Sema5a (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic 10 ug
$823.00

file-alt Citations

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