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Mid1 (BC053704) Mouse Untagged Clone

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SKU
MC217722
Mid1 (untagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Mid1
Synonyms Fxy, 61B3-R, Trim18, MGC60591
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC053704
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAACACTGGAGTCGGAGCTGACCTGTCCTATTTGTCTGGAGCTTTTTGAGGACCCTCTCCTGCTTC
CCTGCGCACATAGCCTCTGCTTCAACTGTGCCCACCGAATCCTGGTCTCTCACTGTGCCACCAACGAGCC
TGTGGAGTCCATCAACGCCTTCCAGTGCCCCACCTGCCGGCATGTCATCACGCCCAGCCAGCGAGGTCTA
GACGGGCTCAAGCGCAACGTCACCCTCCAAAACATTATTGACAGATTTCAGAAAGCATCTGTGAGCGGGC
CCAACTCTCCCAGTGAGACCCGCAGGGAACGGGCCTTTGACGCCGACACCATGTCCTCTGCCGAGAAGGT
TCTCTGCCAGTTCTGTGACCAGGATCCTGCCCAGGATGCTGTGAAGACTTGCGTTACTTGTGAAGTGTCC
TACTGTGATGAGTGCCTGAAAGCCACTCATCCGAACAAGAAGCCCTTTACAGGCCATCGTCTGATTGAGC
CAATCCCGGACTCGCACATCCGGGGGCTGATGTGCCTGGAGCACGAGGATGAGAAGCAAAACTTGGAGAG
TAATCTCACCAATCTTATTAAGAGAAACACAGAACTGGAGACTCTTTTGGCTAAACTCATCCAAACTTGT
CAACATGTTGAAGTCAATGCATCCCGTCAAGAAGCCAAACTGACAGAAGAATGTGATCTTCTCATTGAAA
TCATTCAGCAACGAAGACAAATTATTGGAACAAAGATTAAAGAAGGCAAGGTGATCAGGCTCCGCAAGTT
AGCTCAGCAGATTGCAAACTGTAAACAGTGCCTTGAGAGGTCTGCATCGCTCATCTCGCAAGCGGAGCAC
TCGCTGAAGGAAAATGACCACGCCCGTTTTCTACAGACAGCAAAGAATATCACTGAGAGAGTCTCCATGG
CAACTGCATCCTCCCAGGTCCTAATTCCCGAAATCAACCTCAATGACACGTTTGACACTTTTGCCTTGGA
TTTTTCCCGGGAGAAGAAACTGCTAGAATGTCTGGATTACCTAACAGCTCCCAACCCTCCCGCGATTAGA
GAAGAGCTCTGCACCGCTTCCTACGACACCATCACCGTCCACTGGACCTCAGAGGACGAGTTCAGCGTGG
TCTCCTACGAGCTCCAGTACACCATATTCACCGGACAAGCCAATGTTGTCAGTCTGTGTAACTCGGCGGA
CAGCTGGATGATCGTGCCCAACATCAAGCAGAACCACTACACCGTGTACGGCCTGCAAAGTGGCACCAAG
TATATCTTCACGGTGAAGGCCATCAACCAGGCGGGCAGCCGTAGCAGCGAGCCCGGAAAGCTGAAGACCA
ACAGTTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN BC053704
Insert Size 1338 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC053704, AAH53704
RefSeq Size 3457 bp
RefSeq ORF 1337 bp
Locus ID 17318
Cytogenetics X 79.19 cM
Summary Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.UniProtKB/Swiss-Prot Function
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"BC053704" in other vectors (4)
SKU Description Size Price
MG207113 Mid1 (tGFP-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) 10 ug
$657.00
MR207113 Mid1 (Myc-DDK-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) 10 ug
$289.00 $457.00
MR207113L3 Lenti ORF clone of Mid1 (Myc-DDK-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) 10 ug
$757.00
MR207113L4 Lenti ORF clone of Mid1 (mGFP-tagged) - Mouse midline 1 (cDNA clone MGC:60591 IMAGE:30058736) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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