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Bco1 (NM_001163028) Mouse Untagged Clone

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SKU
MC217460
Bcmo1 (untagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $540.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Bco1
Synonyms Bcd; Bcdo; Bcdo1; Bcmo; Bcmo1; beta-C; beta-CD; betaCM; betaCMOOX; Cm; CMO1; Cmoi
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217460 representing NM_001163028
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGATAATATTTGGCCAGAATAAGAAAGAACAGCTGGAGCCAGTTCAGGCCAAAGTGACAGGCAGCA
TTCCAGCATGGCTGCAGGGGACCCTGCTCCGAAACGGGCCCGGGATGCACACAGTGGGAGAGAGCAAGTA
CAACCATTGGTTTGATGGCCTGGCCCTTCTCCACAGTTTCTCCATCAGAGATGGGGAGGTCTTCTACAGG
AGCAAATACCTGCAGAGTGACACCTACATCGCCAACATTGAGGCCAACAGAATCGTGGTGTCTGAGTTCG
GAACCATGGCCTACCCGGACCCCTGCAAAAACATCTTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT
CCCCGACTTCACAGACAACTGTCTGATCAACATCATGAAATGTGGAGAAGACTTCTATGCAACCACGGAG
ACCAACTACATCAGGAAAATCGACCCCCAGACCCTAGAGACCTTGGAGAAGGTTGATTACCGGAAGTATG
TGGCGGTAAACCTGGCTACCTCGCACCCTCATTATGACGAGGCTGGGAATGTCCTTAACATGGGCACATC
CGTCGTGGACAAAGGGAGGACAAAATACGTGATATTTAAGATCCCTGCCACAGTGCCAGACAGCAAGAAG
AAAGGGAAGAGTCCCGTGAAGCACGCGGAAGTTTTCTGCTCCATTTCCTCCCGCTCGTTGCTCTCTCCCA
GCTACTACCACAGCTTTGGTGTCACGGAGAACTATGTGGTGTTTCTGGAGCAGCCTTTTAAGTTGGATAT
CCTCAAGATGGCCACCGCATACATGAGGGGAGTGAGCTGGGCTTCCTGTATGTCATTCGACAGGGAGGAC
AAGACATACATTCATATCATCGACCAGAGGACCAGGAAGCCTGTGCCTACCAAGTTCTACACAGATCCCA
TGGTGGTCTTCCATCATGTCAATGCCTACGAGGAGGACGGCTGTGTGCTGTTTGATGTGATCGCCTATGA
GGACAGCAGCCTCTATCAGCTCTTCTACCTGGCCAACCTGAACAAGGACTTCGAGGAGAAGTCCAGGCTG
ACCTCAGTGCCTACCCTCAGGAGGTTTGCTGTGCCCCTCCATGTGGACAAGGATGCAGAAGTGGGCTCAA
ATTTAGTCAAGGTGTCATCTACAACTGCAACAGCCCTGAAGGAGAAAGACGGCCATGTCTATTGCCAGCC
CGAGGTCCTCTACGAAGGCCTAGAGCTCCCTCGGATAAATTATGCTTACAACGGGAAGCCATATCGCTAC
ATCTTTGCAGCTGAAGTACAGTGGAGTCCAGTCCCAACCAAGATACTGAAATATGACATTCTCACAAAGT
CCTCCTTAAAGTGGTCTGAGGAGAGCTGCTGGCCAGCAGAGCCTCTGTTTGTTCCCACGCCAGGTGCGAA
GGATGAAGATGATGTCCCTTGCAGGCCAAGCAGAGACTGTGGAGTCCAAGGGCCCCATCGTAAATCACAT
TGTCAGCTGAGCATAGCATGTGTGCCTGAGATCTCAGCATTTGGGAGGCAGACAGAAGACAATCAGGGGT
CTACAGTCATTCTCAGATATACAGAGAATTTGAACTACTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001163028
Insert Size 1581 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001163028.1, NP_001156500.1
RefSeq Size 2532 bp
RefSeq ORF 1581 bp
Locus ID 63857
Cytogenetics 8 E1
Summary Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2009
Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_001163028" in other vectors (4)
SKU Description Size Price
MG227449 Bcmo1 (tGFP-tagged) - Mouse beta-carotene 1515'-monooxygenase (Bcmo1) transcript variant 2, (10ug) 10 ug
$690.00
MR227449 Bcmo1 (Myc-DDK-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2 10 ug
$289.00 $490.00
MR227449L3 Lenti ORF clone of Bcmo1 (Myc-DDK-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2 10 ug
$790.00
MR227449L4 Lenti ORF clone of Bcmo1 (mGFP-tagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2 10 ug
$790.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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