Bco1 (NM_001163028) Mouse Untagged Clone

SKU MC217460

Bcmo1 (untagged) - Mouse beta-carotene 15,15'-monooxygenase (Bcmo1), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Bco1
• Synonyms: Bcd; Bcdo; Bcdo1; Bcmo; Bcmo1; beta-C; beta-CD; betaCM; betaCMOOX; Cm; CMO1; Cmoi
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217460 representing NM_001163028; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGATAATATTTGGCCAGAATAAGAAAGAACAGCTGGAGCCAGTTCAGGCCAAAGTGACAGGCAGCA; TTCCAGCATGGCTGCAGGGGACCCTGCTCCGAAACGGGCCCGGGATGCACACAGTGGGAGAGAGCAAGTA; CAACCATTGGTTTGATGGCCTGGCCCTTCTCCACAGTTTCTCCATCAGAGATGGGGAGGTCTTCTACAGG; AGCAAATACCTGCAGAGTGACACCTACATCGCCAACATTGAGGCCAACAGAATCGTGGTGTCTGAGTTCG; GAACCATGGCCTACCCGGACCCCTGCAAAAACATCTTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT; CCCCGACTTCACAGACAACTGTCTGATCAACATCATGAAATGTGGAGAAGACTTCTATGCAACCACGGAG; ACCAACTACATCAGGAAAATCGACCCCCAGACCCTAGAGACCTTGGAGAAGGTTGATTACCGGAAGTATG; TGGCGGTAAACCTGGCTACCTCGCACCCTCATTATGACGAGGCTGGGAATGTCCTTAACATGGGCACATC; CGTCGTGGACAAAGGGAGGACAAAATACGTGATATTTAAGATCCCTGCCACAGTGCCAGACAGCAAGAAG; AAAGGGAAGAGTCCCGTGAAGCACGCGGAAGTTTTCTGCTCCATTTCCTCCCGCTCGTTGCTCTCTCCCA; GCTACTACCACAGCTTTGGTGTCACGGAGAACTATGTGGTGTTTCTGGAGCAGCCTTTTAAGTTGGATAT; CCTCAAGATGGCCACCGCATACATGAGGGGAGTGAGCTGGGCTTCCTGTATGTCATTCGACAGGGAGGAC; AAGACATACATTCATATCATCGACCAGAGGACCAGGAAGCCTGTGCCTACCAAGTTCTACACAGATCCCA; TGGTGGTCTTCCATCATGTCAATGCCTACGAGGAGGACGGCTGTGTGCTGTTTGATGTGATCGCCTATGA; GGACAGCAGCCTCTATCAGCTCTTCTACCTGGCCAACCTGAACAAGGACTTCGAGGAGAAGTCCAGGCTG; ACCTCAGTGCCTACCCTCAGGAGGTTTGCTGTGCCCCTCCATGTGGACAAGGATGCAGAAGTGGGCTCAA; ATTTAGTCAAGGTGTCATCTACAACTGCAACAGCCCTGAAGGAGAAAGACGGCCATGTCTATTGCCAGCC; CGAGGTCCTCTACGAAGGCCTAGAGCTCCCTCGGATAAATTATGCTTACAACGGGAAGCCATATCGCTAC; ATCTTTGCAGCTGAAGTACAGTGGAGTCCAGTCCCAACCAAGATACTGAAATATGACATTCTCACAAAGT; CCTCCTTAAAGTGGTCTGAGGAGAGCTGCTGGCCAGCAGAGCCTCTGTTTGTTCCCACGCCAGGTGCGAA; GGATGAAGATGATGTCCCTTGCAGGCCAAGCAGAGACTGTGGAGTCCAAGGGCCCCATCGTAAATCACAT; TGTCAGCTGAGCATAGCATGTGTGCCTGAGATCTCAGCATTTGGGAGGCAGACAGAAGACAATCAGGGGT; CTACAGTCATTCTCAGATATACAGAGAATTTGAACTACTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163028
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001163028.1, NP_001156500.1
• RefSeq Size: 2532 bp
• RefSeq ORF: 1581 bp
• Locus ID: 63857
• Cytogenetics: 8 E1
• Summary: Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]; Transcript Variant: This variant (2) includes an alternate exon in the 3' coding region resulting in a frameshift, compared to variant 1. It encodes isoform 2 which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.