Ikzf3 (NM_011771) Mouse Untagged Clone

SKU
MC217154
Ikzf3 (untagged) - Mouse IKAROS family zinc finger 3 (Ikzf3), (10ug)
$709.00
In Stock*
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ikzf3
Synonyms 5830411O07Rik; Aiolos; Zfpn1a3; Znfn1a3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217154 representing NM_011771
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGATATACAACCGACTGTGGAGCTGAAAAGCACGGAGGAGCAGCCTCTGCCCACAGAGAGCCCAG
ACGCTCTGAATGACTACAGCTTGCCCAAACCTCATGAGATAGAAAACGTGGACAGTAGAGAAGCCCCAGC
CAATGAAGACGAAGATGCAGGAGAAGATTCGATGAAAGTGAAAGATGAATACAGCGACAGAGATGAGAAC
ATTATGAAGCCGGAGCCCATGGGAGATGCAGAAGAGAGTGAAATGCCTTACAGCTATGCAAGAGAATACA
GCGACTATGAAAGCATTAAGCTGGAGAGACACGTGCCCTATGACAACAGCAGACCAACCGGTGGGAAGAT
GAACTGCGACGTGTGCGGGTTATCCTGCATTAGCTTCAACGTCTTGATGGTTCATAAGCGAAGCCATACC
GGCGAACGCCCGTTCCAGTGTAATCAGTGCGGGGCATCTTTTACTCAGAAAGGTAACCTCCTCCGTCATA
TTAAACTGCACACGGGGGAAAAACCTTTTAAGTGTCACCTCTGCAACTACGCATGCCAAAGGAGAGATGC
GCTCACGGGACACCTTAGGACACATTCTGTGGAGAAGCCGTACAAGTGTGAGTTCTGCGGAAGAAGCTAC
AAGCAGAGAAGCTCCCTGGAGGAGCACAAGGAACGCTGCCGAGCTTTTCTTCAGAACCCTGACCTGGGGG
ACGCTGCAAGTGTGGAGGCAAGACACATCAAAGCCGAGATGGGAAGTGAGAGAGCTCTCGTCCTGGACAG
ATTAGCAAGCAATGTGGCTAAGCGAAAAAGCTCGATGCCTCAGAAATTCATCGGTGAGAAGCGGCACTGC
TTCGATGCCAACTACAATCCCGGCTACATGTACGAGAAGGAGAACGAGATGATGCAGACCCGGATGATGG
ACCAAGCCATCAATAACGCCATCAGCTATCTAGGGGCTGAAGCCCTTCGCCCCTTAGTCCAGACTCCGCC
TGCTCCCACCTCTGAGATGGTCCCAGTCATCAGCAGTGTGTACCCCATAGCACTTACTCGGGCCGATATG
CCCAATGGGGCCCCGCAGGAGATGGAAAAGAAACGGATCCTCCTGCCAGAGAAGATCTTGCCTTCTGAAC
GAGGTCTGTCCCCCAATAACAGTGCCCAGGACTCCACAGACACCGACAGCAACCACGAGGATCGCCAACA
TCTCTACCAGCAAAGCCACGTGGTCCTCCCCCAGGCCCGCAATGGGATGCCTCTTCTGAAGGAGGTCCCT
CGCTCTTTTGAACTCCTCAAGCCCCCTCCCATCTGCCTGAGGGACTCCATCAAAGTGATCAACAAAGAAG
GGGAGGTGATGGATGTGTTTCGATGTGACCACTGCCACGTCCTCTTCCTAGATTATGTGATGTTCACCAT
CCACATGGGGTGCCATGGTTTCCGTGATCCCTTTGAGTGTAACATGTGTGGCTATCGAAGCCACGATCGC
TATGAGTTCTCCTCTCACATCGCCAGAGGAGAGCACAGAGCCATGTTGAAGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI
ACCN NM_011771
Insert Size 1524 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011771.1, NP_035901.1
RefSeq Size 4098 bp
RefSeq ORF 1524 bp
Locus ID 22780
UniProt ID O08900
Cytogenetics 11 D
Summary Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Binds to GGGAA. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.