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Gja10 (NM_010289) Mouse Untagged Clone

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SKU
MC217137
Gja10 (untagged) - Mouse gap junction protein, alpha 10 (Gja10), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $518.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gja10
Synonyms Cx-57; cx57; Cx59
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC217137 representing NM_010289
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGATTGGAATTTACTGGGTGGCATCCTAGAGGAAGTCCACTCCCACTCCACTATAGTGGGGAAGA
TCTGGCTGACCATCCTCTTCATCTTCCGAATGCTGGTACTTGGTGTCGCTGCTGAGGACGTCTGGGATGA
TGAGCAGTCCGCCTTTGCCTGCAACACCCAGCAGCCCGGTTGCAACAATATCTGTTACGATGATGCTTTC
CCCATCTCTTTGATCAGATTCTGGGTTTTGCAGATCATCTTTGTGTCTTCCCCTTCTTTGGTGTATATGG
GCCATGCCCTTTATAGACTCAGGGACTTTGAGAAGCAGAGGCAGAAGAAGAAGTTATACCTTAGAGCCCA
GATGGAGAATCCAGAGCTCGACCTGGAGGAGCAACAAAGGGTAGATAAAGAGCTGAGGAGACTCGAGGAG
CAGAAGAGGATTCATAAAGTCCCTCTGAAAGGATGTCTGCTGCGCACCTATGTCTTACACATCCTGACCA
GATCAGTGCTAGAAGTAGGGTTCATGATAGGCCAATATATTCTCTATGGGTTTCAAATGCACCCCATTTA
CAAGTGCACCCAAGCCCCCTGCCCCAATTCAGTGGACTGCTTTGTTTCCAGGCCCACAGAGAAGACCATT
TTCATGCTTTTCATGCACAGCATTGCAGCCATCTCCTTGTTACTCAATATCCTGGAAATATTTCATCTCG
GCATCAGGAAAATCATGAGGGCACTCGATGGCAAATCCAGCAGTGGGAACACTGAGAACGAAACAGGCCC
TCCATTCCATTCAACAAACTACTCAGGGACCCAGCAGTGTATGATCTGTTCTTCTTTACCTGAAAGAATC
TCACTACTTCAAGCCAACAATAAACAGCAAGTCATCCGAGTCAATATACCACGGTCTAAAAGCATGTGGC
AAATTCCACACCCCAGGCAACTTGAGGTAGATGTATCCTGTGGCAAAAGAGACTGGGCTGAGAAAATTGA
GAGCTGTGCACAGCTCCACGTCCACAGCCCCTGCCCACATGACCGCAGTGCCAGAATTCAGCACCCTGGA
CAGCAACCGTGCCATTCTGTCTTTGGCCCCAAGAATGCAATGTCTCAGTCTTGGTTCGGTACAATGACGG
CTTCTCAACACCGTCCATCATCTGCGTTAGAAACCTGGGAGCGATCCCAGGGCCCAGAAGCTTCAGGGAG
ATCTCTCACAGATCGCCAGAGTCACTTCCAAGGCAGTGACGGCAGTGCAAGAGAGAGTGGGGTTTGGACA
GACAGATTAGGCCCAGGAAGTCGCAAGGCCAGCTTTCTATCGAGGCTGATGTCAGAAAAGGGACAACGGC
ATAGTGACTCAGGAAGCTCACGGTCTCTGAATAGTTCCTGCTTGGATTTTTCACACGGAGAAAATAGCCC
ATCACCTCTGCCGTCTGCCACTGGGCACAGAGCATCGATGGTAAGTAAAAGCAGCCATGTTGATTCACCT
CCTCACTCTTCTTTCATCATACATGAGACATATGTATATGTGTATTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010289
Insert Size 1518 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010289.2, NP_034419.2
RefSeq Size 1518 bp
RefSeq ORF 1518 bp
Locus ID 14610
UniProt ID Q9WUS4
Cytogenetics 4 14.25 cM
Summary One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Involved in tracer coupling between horizontal cells of the retina. May play a role in the regulation of horizontal cell patterning.UniProtKB/Swiss-Prot Function
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Documents
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"NM_010289" in other vectors (4)
SKU Description Size Price
MG223519 Gja10 (tGFP-tagged) - Mouse gap junction protein alpha 10 (Gja10), (10ug) 10 ug
$670.00
MR223519 Gja10 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 10 (Gja10) 10 ug
$289.00 $470.00
MR223519L3 Lenti ORF clone of Gja10 (Myc-DDK-tagged) - Mouse gap junction protein, alpha 10 (Gja10) 10 ug
$770.00
MR223519L4 Lenti ORF clone of Gja10 (mGFP-tagged) - Mouse gap junction protein, alpha 10 (Gja10) 10 ug
$770.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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