Srf (NM_020493) Mouse Untagged Clone

SKU MC217127

Srf (untagged) - Mouse serum response factor (Srf), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Srf
• Synonyms: AW049942; AW240594
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC217127 representing NM_020493; Red=Cloning site Blue=ORF; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTACCGAGCCAAGCTGGGGCCGCGGCGGCTCTGGGCCGGGGCTCGGCCCTGGGGGGCAACCTGAACC; GGACCCCGACGGGGCGGCCGGGCGGCGGCGGCGGGACTCGCGGGGCGAACGGGGGCCGGGTCCCCGGGAA; CGGCGCGGGGCTCGGCCAGAGTCGTCTGGAGCGGGAGGCTGCAGCGGCAGCGGCGCCCACCGCCGGGGCC; CTCTACAGCGGCAGCGAGGGCGACTCCGAGTCCGGCGAGGAGGAGGAGCTGGGCGCCGAGCGGCGCGGCC; TCAAGCGGAGCCTGAGCGAGATGGAGCTCGGCGTGGTGGTCGGTGGGCCTGAGGCGGCGGCGGCGGCCGC; CGGGGGCTACGGGCCGGTGAGCGGCGCGGTGAGCGGGGCCAAGCCGGGGAAGAAGACCCGGGGCCGCGTG; AAGATCAAGATGGAGTTCATCGACAACAAGCTGCGGCGCTACACGACCTTCAGCAAGAGGAAGACGGGCA; TCATGAAGAAGGCCTATGAGCTGTCCACGCTGACAGGGACACAGGTGCTGTTGCTGGTGGCCAGTGAGAC; AGGCCATGTGTATACCTTTGCCACCCGCAAACTGCAGCCCATGATCACCAGTGAGACCGGCAAGGCGCTG; ATTCAGACCTGCCTCAACTCGCCAGACTCTCCGCCCCGCTCAGACCCCACCACAGACCAGAGAATGAGTG; CCACTGGCTTTGAAGAGCCAGATCTCACCTACCAGGTGTCGGAATCTGACAGCAGTGGGGAAACCAAGGA; CACACTGAAGCCAGCATTCACAGTCACCAACCTGCCGGGTACCACCTCCACAATCCAGACAGCACCCAGC; ACCTCTACCACCATGCAAGTCAGCAGCGGCCCCTCCTTCCCCATCACCAACTACCTGGCACCAGTGTCTG; CTAGTGTCAGCCCCAGCGCTGTCAGCAGTGCCAACGGGACTGTGCTCAAGAGTACAGGCAGCGGCCCTGT; CTCCTCTGGGGGCCTTATGCAGCTGCCTACCAGCTTCACCCTCATGCCTGGTGGGGCAGTGGCCCAGCAG; GTCCCTGTGCAGGCCATTCATGTGCACCAGGCCCCACAGCAAGCGTCTCCCTCTCGTGACAGCAGCACAG; ACCTCACGCAGACCTCCTCCAGCGGGACAGTGACGTTGCCCGCCACCATCATGACGTCGTCTGTACCCAC; AACTGTGGGTGGCCACATGATGTACCCTAGTCCCCATGCAGTGATGTATGCCCCCACCTCAGGCCTGGCT; GATGGCAGCCTCACCGTGCTCAATGCCTTCTCTCAGGCACCATCCACCATGCAGGTGTCCCACAGCCAGG; TCCAGGAGCCAGGTGGTGTCCCTCAGGTGTTCCTGACAGCACCGTCTGGGACCGTGCAGATCCCTGTCTC; TGCAGTTCAGCTTCACCAGATGGCTGTGATAGGGCAGCAAGCTGGGAGCAGCAGCAACCTCACCGAGCTA; CAGGTGGTGAACCTGGATGCCACCCACAGCACCAAGAGTGAATGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_020493
• Insert Size: 1515 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC051950, AAH51950
• RefSeq Size: 2634 bp
• RefSeq ORF: 1515 bp
• Locus ID: 20807
• UniProt ID: Q9JM73
• Cytogenetics: 17 C
• Summary: SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (PubMed:24732378). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration (PubMed:12732141, PubMed:19350017, PubMed:24732378). The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics (PubMed:24732378). Required for cardiac differentiation and maturation (PubMed:15169892).[UniProtKB/Swiss-Prot Function]