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Trmt6 (NM_175113) Mouse Untagged Clone

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SKU
MC216936
Trmt6 (untagged) - Mouse tRNA methyltransferase 6 homolog (S. cerevisiae) (Trmt6), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trmt6
Synonyms 3300001M20Rik; AU016412; AW492787; CGI-09; mKIAA1153
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216936 representing NM_175113
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCATCAGCTGCGGAGCAGCCGAGCTCACCGCCCCCGCCTCTCGGGGACCACTGCATCCATGACG
GTGACTTCGTGGTGCTGAAGCGAGAAGATGTGTTCAAAGCAGTGCAAGTCCAGCGGAGAAAAAAAGTAAC
TTTTGAAAAGCAGTGGTTCTACCTGGATAACGCCATCGGCCATAGTTACGGCTCAGCGTTTGACGTGAGC
AGTGGAGGCAGCCTTCAGCTCAGGAAGAAGCTGGAGGAGCCCGCGTCAGAGACCAAAGAAGCAGGCACTG
ATAATCGAAATATAGTTGATGATGGAAAGTCCCAGAAACTTACTCAAGATGATATAAAAGCTCTGAAAGA
CAAGGGCATTAAAGGAGAGGAAATAGTTCAGCAGCTAATTGAAAATAGTACAACATTCCGTGACAAGACA
GAATTTGCCCAAGATAAATATATTAAAAAGAAGAAGAAAAAATATGAAGCCATCGTTACTATCTTGAAGC
CATCTACCCGTATTCTTTCAATTATGTATTATGCAAGAGAACCTGGAAAAATTAACCACATGAGATATGA
TACACTAGCCCAGATGTTGACGTTGGGAAATATCCGTGCCGGCAATAAAATGATTGTCATGGAAACGTGC
TCGGGCTTGGTGCTAGGTGCCATGATGGAACGAATGGGAGGCTTTGGCTCCATTATTCAGCTGTATCCTG
GAGATGGACCCGTTCGGGCGGCAACAGCATGTTTTGGATTTCCCAAATCTTTCCTCAGTGGTCTTTACGA
GTTCCCTCTCAACAAAGTAAACAGTCTCCTCAATGGGACATTTTCTGCTGAGATGCTGTCCTCAGAGCCT
AAAGACAGCACTCCAGTTGAAGAAAGTAACGGTGAGCTTGAGGAGAAAGAGATTGCTGAACAAGCGGATG
AAGACAACATTGTGGATGCCGCAGAAAACAACTCAGGAGAACAGAGACCAATGGAGATTGTTCCTGGGGA
CCCAGAGAATAAGGAGCCCAAAGAAAAAAGAAGCAAAAGAGATTATATTCAGGAAAAGCAAAGGAGACAA
GAAGAGCAGAGGAAAAGACATCTGGAGGCTGCTGCTCTGCTGGGAGAAAGAAATGCAGATGGTTTGATTG
TGGCCAGTCGTTTCCACCCCACGCCCCTGCTGCTGTCTTTGCTGGACTTTGTAGCCCCGTCAAGGCCGTT
TGTGGTCTACTGTCAGTATAAAGAGCCTTTGTTGGAATGCTACACAAAACTTCGGGAGAGGGGAGGAGTC
ATTAACCTGAGGTTGTCTGAAACCTGGCTCAGAAATTACCAGGTTCTGCCAGATCGGAGTCATCCCAAAT
TATTGATGAGTGGCGGTGGAGGGTACCTTCTGTCAGGGTTCACTGTTGTCTCAGACAGCCTTCGGGCAGA
CCCCAGCCTCAAGTCCTGCACAGGCGCTTTAGACCCACACAAGGCTGAGGAGCCAGCAGCTAAAAAACAG
AAATGCATGGAATCTGCCTCTTAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_175113
Insert Size 1494 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_175113.3, NP_780322.2
RefSeq Size 2820 bp
RefSeq ORF 1494 bp
Locus ID 66926
UniProt ID Q8CE96
Cytogenetics 2 F2
Summary Substrate-binding subunit of tRNA (adenine-N(1)-)-methyltransferase, which catalyzes the formation of N(1)-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Together with the TRMT61A catalytic subunit, part of a mRNA N(1)-methyltransferase complex that mediates methylation of adenosine residues at the N(1) position of a small subset of mRNAs: N(1) methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longest isoform (1).
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Documents
Resources
"NM_175113" in other vectors (3)
SKU Description Size Price
MR207979 Trmt6 (Myc-DDK-tagged) - Mouse tRNA methyltransferase 6 homolog (S. cerevisiae) (Trmt6) 10 ug
$289.00 $457.00
MR207979L3 Lenti ORF clone of Trmt6 (Myc-DDK-tagged) - Mouse tRNA methyltransferase 6 homolog (S. cerevisiae) (Trmt6) 10 ug
$757.00
MR207979L4 Lenti ORF clone of Trmt6 (mGFP-tagged) - Mouse tRNA methyltransferase 6 homolog (S. cerevisiae) (Trmt6) 10 ug
$757.00

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