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Sesn3 (NM_030261) Mouse Untagged Clone

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SKU
MC216865
Sesn3 (untagged) - Mouse sestrin 3 (Sesn3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sesn3
Synonyms 5630400E15Rik; BC003348; SEST3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216865 representing NM_030261
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGCGGTGGCAGCAGCGCGTCGGCCTCGGCCAACTACCTGCTCTGCACCAACTGCCGGAAAGTGC
TGCGGAAGGACAAAAGAATCCGAGTGTCTCAACCCCTGACAAGAGGACCAAGTGCCTTTATTCCAGAGAA
GGAAGTTGTCCAAGCAAATACGGCGGATGAACGAACAAACTTTCTTGTGGAAGAGTACTCTACATCCGGT
CGCCTGGACAACATCACACAGGTGATGAGCTTGCACACTCAGTACCTGGAATCTTTCTTGCGGAGCCAGT
TCTACATGCTGCGTATGGACGGCCCCCTTCCCCTCCCAGACAGGCACTACATTGCCATCATGGCTGCAGC
CAGACACCAGTGTTCCTACCTAATAAACATGCATGTGGATGAATTTTTAAAGACTGGAGGGATCGCTGAG
TGGCTGAATGGCTTGGAATATGTACCACAAAGACTGAGAAATCTTAATGAAATTAACAAGCTGCTGGCAC
ACCGGCCCTGGCTGATCACAAAAGAGCACATTCAGAAACTTGTCAAAACTGGAGAAAATAATTGGTCCCT
GCCTGAGCTAGTGCACGCCGTGGTCCTCTTGGCTCACTATCACGCTTTGGCCAGCTTTGTTTTTGGTAGT
GGCATCAATCCAGAGAGAGACCCAGGAATCGCCAATGGGTTCAGACTAATCTCTGTGAGCAGCTTCTGTG
TGTGTGACCTGGCCAATGACAACAGCATCGAGAACACCTCCCTGGCGGGCAGCAACTTTGGGATTGTGGA
TTCCCTAGGCGAGCTGGAAGCCTTAATGGAAAGGATGAAAAGGCTTCAGGAAGACAGGGAAGATGACGAG
ACCACTCGGGAAGAAATGACCACGCGTTTTGAGAAGGAAAAGAAAGAAAGTCTCTTTGTGGTCCCTGGAG
AAACTTTACATGCGTTTCCTCACTCAGATTTTGAAGATGATGTGATTGTAACAGCCGATGTCTCTCGATA
CATTGAAGACCCAAGCTTTGGGTACGAAGACTTTGCCAGGCGAGGAGAAGAACATTTGCCAACATTCCGA
GCCCAGGACTACACCTGGGAAAACCATGGGTTCTCACTGGTGAACAGGCTTTACTCTGACATTGGACATC
TCCTGGATGAGAAGTTTCGAATGGTCTACAATCTCACCTACAACACAATGGCCACCCATGAGGATGTTGA
CACGACCACACTGCGCAGAGCTTTGTTTAACTATGTTCACTGTATGTTTGGAATCAGGTATGATGACTAT
GACTATGGAGAAGTTAACCAGTTACTTGAACGGAGCCTGAAGGTTTACATCAAGACAGTGACCTGCTACC
CTGAGAGAACCACGAAGCGCATGTATGACAGCTACTGGCGCCAGTTCACTCACTCAGAGAAGGTCCACGT
GAATCTGCTTCTGATGGAAGCACGGATGCAGGCCGAGCTGCTTTATGCTCTGCGAGCCATAACTCGGCAT
CTGACCTGA

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII |
ACCN NM_030261
Insert Size 1479 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030261.4, NP_084537.2
RefSeq Size 2158 bp
RefSeq ORF 1479 bp
Locus ID 75747
UniProt ID Q9CYP7
Cytogenetics 9 A1
Summary May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25259925). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (PubMed:25377878). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_030261" in other vectors (4)
SKU Description Size Price
MG220914 Sesn3 (tGFP-tagged) - Mouse sestrin 3 (Sesn3), (10ug) 10 ug
$657.00
MR220914 Sesn3 (Myc-DDK-tagged) - Mouse sestrin 3 (Sesn3) 10 ug
$289.00 $457.00
MR220914L3 Lenti ORF clone of Sesn3 (Myc-DDK-tagged) - Mouse sestrin 3 (Sesn3) 10 ug
$757.00
MR220914L4 Lenti ORF clone of Sesn3 (mGFP-tagged) - Mouse sestrin 3 (Sesn3) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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