Pramel7 (NM_178250) Mouse Untagged Clone

SKU
MC216751
Pramel7 (untagged) - Mouse preferentially expressed antigen in melanoma like 7 (Pramel7), (10ug)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Pramel7
Synonyms AA675346; C81180
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216751 representing NM_178250
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCAGTGCCCCACCCACACTCCAGGATCAGGCTTTCAGGAGCCTGGTGAGGAATGAAGTATTGACCG
TCTCTGATGCGGAGTGCCTAATAAGGGAATTCTTCCCACCTCTGTTCAAGGAGGCCAGCACTCAAAAAAA
ACCAAAGACAATTATGATCCTGGTGGAGCACTGGCCCTACCCTTGCCTTCATGTTGGGTTGTTGATTGAT
AAACCCAACTTTCAGATCTTCCAGGCTATACTGGATGGAGTAGACACATGGCTGAAACGGAAGTATCGCC
CCAGGATGGGAAGGCTTAAAAAGGTTGACTTCAGGGATGCACAGCATCATGCTTCCTTAGATATGCAGGA
TGAGAGAGAGGGTAGAGACTACTTAGTAGGAACTTTGCCTAAGAAGCAAATAGTGGAGGACCACTCAAGA
ACAAGGAAAGAGCGTTTGAAGCTGTTCCATGACCTTTCCTTTATGTCTTCTCTGCATGAGGATAAACACC
AAACACTGTTATTGGAGTGGGCAAAGGAAAGGACGAGTTTCCTGCATCTGTGCTGTGAGAAGTTGGAGAT
AGGAGCAGTAGAAGTGTCTAAGGTCAGGAATGTATTAAAATTTCTACAGCCAGAGCTCATCAAGGAGTTG
AAACTGAATACAGTGGGGAATCTGTCCAAACTGGCAAAATTCGTACCTTTCATTAGAAAGATGAGAAACC
TTCAGAAACTCATGCTAGTACGCACCTTTGGAACAAGGACTTTCACTCAAGAGGAGAAGCAGAACATCAG
CAAGATCATTTCTCTGTTCTGCAAACTCAGCTGTCTCCGGCATCTCACTATTGATGATGTCTATTTTCTC
ACAGATCAGATGAAAGAGTTGCTCAGGTGTCTGGAGGCCCCATTGGTGTCCCTCAAGATCACCCTGTGCC
AGCTCTCTCAGTCAGACTTGGAGTCATTTGCCCAGCGCTGGAACTACAGTCAGCTTAAACATCTATGCTT
GAGAGGTGTCACTTTAACTAACCTAGATGTCACGCCTCTAAGAGATTTCTTAAAGCGTGTTGCAGCTAAC
CTGCAGACTCTGGACTTAGAAGACTGTAGGATGGATGATTCTCACTTCAGGACCCTTCTTCCTGCCCTGA
TCAAATGCACCCAGCTCACTAGCATCAATTTGTATGACAATGACATCTCCGAGGATGTCCTGGAGAACTT
TTTGCACCGCACTACCAATCTGAGCCAGCTGACCACAGAAATGTATCCTGCTCCATCAGAGGTCTATAAT
GAGTCCAACTATGTTATAGTAGAGATATTTATTCAGATTTGTTCTGAACTTATGAACAAACTCATGGAAG
TCAGGCAGGCCAATTCAGTGTGCTTTGGTTCCTCTTCCTGTTATGACTGTGATAATCGTTATTTATATGA
AGATGATGGGGATGTCACACTTTGTTTGTGCCAGGAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_178250
Insert Size 1440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178250.2, NP_839981.1
RefSeq Size 1860 bp
RefSeq ORF 1440 bp
Locus ID 347712
UniProt ID Q810Y8
Cytogenetics 2 D
Summary Promotes maintenance and self-renewal of pluripotent embryonic stem cells (ESCs), downstream of LIF/STAT3 (PubMed:21425410). Maintains the pluripotency state of ESCs by repressing DNA methylation through the regulation of UHRF1 stability. Mediates the proteasomal degradation of UHRF1. Is required for the establishment of the blastocyst (PubMed:28604677).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_178250" in other vectors (4)
SKU Description Size Price
MG213311 Pramel7 (tGFP-tagged) - Mouse preferentially expressed antigen in melanoma like 7 (Pramel7), (10ug) 10 ug
$932.00
MR213311 Pramel7 (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma like 7 (Pramel7) 10 ug
$732.00
MR213311L3 Lenti ORF clone of Pramel7 (Myc-DDK-tagged) - Mouse preferentially expressed antigen in melanoma like 7 (Pramel7) 10 ug
$1,032.00
MR213311L4 Lenti ORF clone of Pramel7 (mGFP-tagged) - Mouse preferentially expressed antigen in melanoma like 7 (Pramel7) 10 ug
$1,032.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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