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Gfra2 (NM_008115) Mouse Untagged Clone

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SKU
MC216255
Gfra2 (untagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 2 (Gfra2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Gfra2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216255 representing NM_008115
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATCTTGGCAAACGCCTTCTGCCTCTTCTTCTTTTTAGACGAAACCCTCCGCTCTTTGGCCAGCCCTT
CCTCTCCGCAGGGCTCTGAGCTCCACGGCTGGCGCCCCCAAGTGGACTGTGTCCGGGCCAATGAGCTGTG
TGCGGCTGAATCCAACTGCAGCTCCAGGTACCGCACCCTTCGGCAGTGCCTGGCAGGCCGGGATCGCAAT
ACCATGCTGGCCAATAAGGAGTGCCAGGCGGCCCTGGAGGTCTTGCAGGAAAGCCCATTGTATGACTGCC
GCTGCAAGCGGGGCATGAAGAAGGAGCTGCAGTGTCTGCAGATCTATTGGAGCATCCATCTGGGGCTGAC
GGAGGGTGAGGAGTTCTACGAAGCTTCGCCCTATGAGCCTGTGACCTCCCGCCTCTCGGACATCTTCAGG
CTCGCTTCAATCTTCTCAGGGACAGGGGCAGACCCGGTGGTCAGTGCCAAGAGCAACCACTGCCTGGATG
CCGCCAAGGCCTGCAACCTGAACGACAACTGCAAGAAGCTCCGCTCCTCCTACATCTCCATCTGCAACCG
CGAGATCTCTCCCACCGAACGCTGCAACCGCCGCAAGTGCCACAAGGCCCTGCGCCAGTTCTTCGACCGT
GTGCCCAGCGAGTATACCTACCGCATGCTCTTCTGCTCCTGTCAGGACCAGGCATGCGCCGAGCGTCGCC
GGCAAACCATCCTGCCCAGCTGTTCCTATGAGGACAAAGAGAAGCCCAATTGCTTGGACCTGCGCAGCCT
GTGTCGTACAGACCACTTGTGCCGGTCCCGCCTGGCAGACTTCCACGCCAACTGTCGAGCCTCCTACCGG
ACAATCACCAGCTGCCCTGCGGACAACTACCAGGCATGTCTGGGCTCCTATGCTGGCATGATTGGGTTTG
ATATGACACCGAACTATGTGGACTCCAACCCCACGGGCATCGTGGTGTCTCCCTGGTGCAATTGTCGTGG
CAGTGGGAACATGGAAGAAGAGTGTGAGAAGTTCCTCAAGGACTTCACAGAAAACCCATGCCTCCGGAAT
GCCATTCAAGCCTTTGGCAATGGCACAGATGTGAACATGTCTCCCAAAGGCCCCACATTTTCAGCTACCC
AGGCCCCTCGGGTAGAGAAAACTCCTTCACTGCCAGATGACCTCAGTGATAGCACCAGCCTGGGGACCAG
TGTCATCACCACCTGCACATCTATCCAGGAGCAAGGGCTGAAGGCCAACAACTCCAAAGAGTTAAGCATG
TGTTTCACAGAGCTCACGACAAATATCAGCCCAGGGAGTAAAAAGGTGATCAAACTTTACTCAGGCTCCT
GCAGAGCCAGACTGTCGACTGCCTTGACTGCCCTCCCACTCCTGATGGTGACCTTGGCCCAGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_008115
Insert Size 1395 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008115.3, NP_032141.2
RefSeq Size 3487 bp
RefSeq ORF 1395 bp
Locus ID 14586
UniProt ID O08842
Cytogenetics 14 D2
Summary The protein encoded by this gene is part of the receptor complex that transduces glial cell-derived neurotrophic factor and neurturin signals by mediating autophosphorylation and activation of the RET receptor. Mice lacking this protein are viable and fertile but display growth retardation attributed to impaired salivary and pancreatic secretion and innervation deficits in the intestinal tract. In addition, knockout mice display neural defects including a failure to initiate outgrowth of dorsal ganglion root neurons, demonstrating a requirement in neuronal differentiation of these cells. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Oct 2014
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).
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Documents
Resources
"NM_008115" in other vectors (4)
SKU Description Size Price
MG225280 Gfra2 (tGFP-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 2 (Gfra2), (10ug) 10 ug
$657.00
MR225280 Gfra2 (Myc-DDK-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 2 (Gfra2) 10 ug
$289.00 $457.00
MR225280L3 Lenti ORF clone of Gfra2 (Myc-DDK-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 2 (Gfra2) 10 ug
$757.00
MR225280L4 Lenti ORF clone of Gfra2 (mGFP-tagged) - Mouse glial cell line derived neurotrophic factor family receptor alpha 2 (Gfra2) 10 ug
$757.00

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