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Def8 (NM_054046) Mouse Untagged Clone

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SKU
MC215979
Def8 (untagged) - Mouse differentially expressed in FDCP 8 (Def8), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Def8
Synonyms AI449518; D8Ertd713e; DEF-8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC215979 representing NM_054046
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATATGATGAGAAGCTGGTTCGGTTCCGGCAGGCCCACCTCAACCCCTTCAACAAGCAGCTTGGTC
CGAGGCATCATGAACAGGAACCCAGTGAGAAGGTCACTTCTGAAGACACTTTGCCTGAGCTGCCCGCTGG
GGAGCCTGAATTCCACTACTCGGAGCGCATGATGGATCTCGGCCTGTCTGAGGACCACTTTTCCCGCCCT
GTGGGTCTCTTCCTGGCCTCTGATGTCCAGCAGCTGCGGCAGGCCATCGAAGAATGCAAACAGGTGATCC
TGGAGCTGCCCGAGCAGTCAGAGAAGCAGAAGGACGCTGTGGTGCGGCTGATCCACCTCCGGCTGAAGCT
CCAGGAGCTGAAGGACCCCAATGAGGAGGAACCCAACATCCGGGTTCTCCTGGAACATCGCTTCTACAAG
GAGAAGAGCAAGAGCGTTAAACAGACCTGCGATAAGTGCAACACCATCATCTGGGGGCTCATCCAGACCT
GGTACACCTGCACAGGGTGTTGTTACCGCTGTCACAGCAAGTGCCTGAACCTCATCTCCAAACCATGTGT
CAGCTCCAAGGTCAGTCACCAGGCTGAGTATGAGCTGAACATCTGCCCTGAGACCGGGCTGGACAGCCAG
GACTACCGCTGTGCGGAGTGCCGTGCTCCCATCTCTCTGAGAGGTGTGCCTAGTGAGGCCCGGCAGTGTG
ACTACACGGGCCAATACTACTGCAGCCACTGCCACTGGAACGACCTGGCTGTCATCCCCGCGAGAGTGGT
GCACAACTGGGACTTTGAGCCACGCAAGGTGTCCCGTTGCAGCATGCGCTACCTGGCACTGATGGTGTCT
CGGCCAGTGCTCCGGCTCCGGGAGATTAACCCTTTGCTGTTTAACTACGTGGAAGAGCTGGTGGAGATCC
GGAAACTGCGCCAGGACATCCTGCTCATGAAGCCATACTTCATCACCTGCAAGGAGGCCATGGAGGCGCG
ACTACTGCTGCAGCTCCAAGACAGACAGCATTTTGTGGAGAACGATGAGATGTACTCTATCCAGGACCTC
TTGGAAGTGCACATGGGCCGCCTCAGCTGCTCGCTCACTGAGATCCACACGCTCTTCGCCAAGCACATCA
AGTTGGACTGTGAGCGGTGCCAAGCCAAGGGGTTCGTCTGTGAACTCTGCAAAGAAGGGGACGTGCTCTT
CCCGTTCGACAGCCACACGTCTGTGTGCAATGACTGTTCGGCTGTCTTCCACAGGGACTGTTACTACGAC
AACTCGACCACGTGCCCCAAGTGTGCCCGGCTCACATTGAGGAAGCAGTCACTATTCCAGGAACCTGGCC
TAGACATGGATGCCTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_054046
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_054046.5, NP_473387.1
RefSeq Size 3786 bp
RefSeq ORF 1347 bp
Locus ID 23854
UniProt ID Q99J78
Cytogenetics 8 72.1 cM
Summary Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (PubMed:27777970). Involved in bone resorption (PubMed:27777970).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (3) lacks two alternate exons in the coding region that results in the use of a downstream start codon compared to variant 1. It encodes isoform 3 which has a shorter N-terminus compared to isoform 1. Both variants 3 and 4 encode the same isoform (3).
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Documents
Resources
"NM_054046" in other vectors (4)
SKU Description Size Price
MG207144 Def8 (tGFP-tagged) - Mouse differentially expressed in FDCP 8 (Def8) 10 ug
$657.00
MR207144 Def8 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 8 (Def8) 10 ug
$289.00 $457.00
MR207144L3 Lenti ORF clone of Def8 (Myc-DDK-tagged) - Mouse differentially expressed in FDCP 8 (Def8) 10 ug
$757.00
MR207144L4 Lenti ORF clone of Def8 (mGFP-tagged) - Mouse differentially expressed in FDCP 8 (Def8) 10 ug
$757.00

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