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Ankrd16 (NM_177268) Mouse Untagged Clone

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SKU
MC214483
Ankrd16 (untagged) - Mouse ankyrin repeat domain 16 (Ankrd16), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Ankrd16
Synonyms 2810455F06Rik; AI646698; D430029B21Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214483 representing NM_177268
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCTGCCTGGGGATCCGCGGCGCCTCTGCAGGCTGGTGCAAGAGGGCCGACTGCGTGACCTTCAGG
AGGAACTGGCGGTAGCTAGAGGTTGCCGGGGGCCAGCCGGAGACACCCTTCTCCACTGTGCAGCACGCCA
CGGACGCCAGGATATCCTAGCGTACCTAGTGGAGGCTTGGAGTATGGACATCGAGGCTACCAACCGAGAC
TACAAGCGGCCTCTGCACGAAGCTGCCTCTATGGGCCACCGGGACTGCGTGCGCTACCTCCTGGGCCGAG
GTGCAGTCGTGGACTCCTTGAAGAAGGCGGACTGGACTCCTCTGATGATGGCGTGCACAAGGAAGAACCT
TGATGTGATCCAGGACCTTGTAGAACACGGTGCCAATCCACTCCTGAAGAACAAGGATGGCTGGAACAGT
TTCCACATTGCCAGTAGAGAAGGCCACCCTGTGATCCTCCGGTACTTGCTCACTGTCTGCCCTGATGCTT
GGAAAACAGAGAGCAACATTAGAAGAACCCCTTTACACACTGCAGCAATGCACGGCTGTTTGGAAGCAGT
CCAGGTGCTTCTTGAAAGGTGTCACTATGAACCAGACTGTCGAGACAACTGTGGTGTCACGCCCTTCATG
GATGCAATTCAGTGTGGCCATGTTAGTATAGCCAAGCTGCTCCTTGAACAGCATAAGGCTTGCTCTTCAG
CTGCAGATAGCATGGGGGCCCAGGCTCTACACCGCGCAGCAGTCACTGGGCAGGATGAAGCCATACGGTT
CCTGGTATGCGGTCTTGGCATCGATGTAGATGTAAGAGCAAAGTCAAGCCAGCTCACAGCACTTCACTAT
GCAGCAAAGGAAGGACAGACGAATACAGTTCAAACTCTGTTGTCCTTGGGTGCCGACATCAACTCTACAG
ATGAAAGAAATCGCTCAGTCCTGCATCTGGCCTGCGCAGGTCAGCATGTGGCTTGCACCAGGCTCCTCCT
ACAGTCGGGACTGAAGGATTCCGAAGACCTCACAGGCACCTTGGCCCAGCAGCTCACGAGAAGCGTAGAT
ATCCTTCAGGACTTTGACCATGACGTGAAATCGTAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_177268
Insert Size 1086 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177268.4, NP_796242.2
RefSeq Size 2356 bp
RefSeq ORF 1086 bp
Locus ID 320816
UniProt ID A2AS55
Cytogenetics 2 A1
Summary Required to prevent the misactivation of serine (Ser) with tRNA(Ala) by promoting the hydrolysis of Ser-mischarged tRNA(Ala), thereby playing a role in translational fidelity (PubMed:29769718). Binds directly to the catalytic domain of AARS/AlaRS and captures Ser that is misactivated by AARS/AlaRS, preventing the charging of Ser adenylates to tRNA(Ala) and precluding Ser misincorporation in nascent peptides (PubMed:29769718).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
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Documents
Resources
"NM_177268" in other vectors (4)
SKU Description Size Price
MG219138 Ankrd16 (tGFP-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16), (10ug) 10 ug
$657.00
MR219138 Ankrd16 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) 10 ug
$289.00 $457.00
MR219138L3 Lenti ORF clone of Ankrd16 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) 10 ug
$757.00
MR219138L4 Lenti ORF clone of Ankrd16 (mGFP-tagged) - Mouse ankyrin repeat domain 16 (Ankrd16) 10 ug
$757.00

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