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March3 (NM_177115) Mouse Untagged Clone

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SKU
MC214450
41336 (untagged) - Mouse membrane-associated ring finger (C3HC4) 3 (March3), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol March3
Synonyms 6330411I15Rik; A530081L18Rik; AI843639; MARCH-III
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214450 representing NM_177115
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAACCAGTCGCTGCAGTCACCTGCCCGAAGTCTTGCCAGACTGCACCAGCTCAGCCGCGCCTGTGG
TGAAGACGGTGGAGGATTGTGGCAGCCTAGTGAATGGGCAGCCACAGTATGTCATGCAAGTTTCAGCCAA
GGACGGGCAGCTGCTGTCAACAGTAGTGCGGACTCTTGCCACCCAGAGCCCCTTCAATGACCGGCCAATG
TGCAGGATCTGCCATGAGGGAAGCAGCCAAGAGGACTTACTCTCTCCGTGTGAATGTACTGGGACCTTGG
GGACCATCCATCGGAGCTGCCTGGAGCACTGGCTGTCATCCTCAAATACCAGCTACTGTGAACTCTGCCA
CTTCAGGTTTGCAGTGGAGCGCAAACCCAGGCCGTTAGTGGAGTGGCTTAGAAACCCTGGCCCCCAGCAT
GAGAAGCGGACTCTGTTTGGAGATATGGTGTGCTTCTTGTTTATAACGCCCCTGGCTACCATCTCAGGCT
GGCTGTGCCTGCGGGGGGCCGTGGATCACCTGCACTTTAGTAGCCGGCTGGAGGCCGTCGGACTCATTGC
CCTCACTGTGGCACTCTTCACCATTTACCTCTTCTGGACACTAAGGCGTTATGGACACCAAAGTAAACCA
TTTTGGAATCAGTCGAGCAGAAGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_177115
Insert Size 657 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177115.2, NP_796089.1
RefSeq Size 1754 bp
RefSeq ORF 657 bp
Locus ID 320253
UniProt ID Q8BRX9
Cytogenetics 18
Summary E3 ubiquitin-protein ligase which may be involved in endosomal trafficking. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.UniProtKB/Swiss-Prot Function
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"NM_177115" in other vectors (4)
SKU Description Size Price
MG216254 41336 (tGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 3 (March3), (10ug) 10 ug
$650.00
MR216254 41336 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 3 (March3) 10 ug
$450.00
MR216254L3 Lenti ORF clone of 41336 (Myc-DDK-tagged) - Mouse membrane-associated ring finger (C3HC4) 3 (March3) 10 ug
$750.00
MR216254L4 Lenti ORF clone of 41336 (mGFP-tagged) - Mouse membrane-associated ring finger (C3HC4) 3 (March3) 10 ug
$750.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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