H2ac8 (NM_178187) Mouse Untagged Clone

SKU
MC214375
Hist1h2ae (untagged) - Mouse histone cluster 1, H2ae (Hist1h2ae), (10ug)
  $165.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol H2ac8
Synonyms 1190022L06; Hist1h; Hist1h2ae
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC214375 representing NM_178187
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGACGTGGCAAACAGGGTGGCAAGGCTCGCGCCAAGGCCAAGACCCGCTCCTCCCGGGCCGGCC
TGCAGTTTCCCGTGGGCCGCGTGCACCGGCTGCTCCGCAAAGGCAACTACTCGGAGCGCGTGGGCGCCGG
CGCCCCGGTGTACCTGGCGGCCGTGCTGGAGTACCTGACGGCCGAGATCCTGGAGCTGGCGGGCAACGCG
GCCCGCGACAACAAGAAGACGCGCATCATCCCGCGCCACCTGCAGCTGGCCATCCGCAACGACGAGGAGC
TCAACAAGCTGCTGGGCCGCGTGACCATCGCGCAGGGCGGCGTCCTGCCCAACATCCAGGCCGTGCTGCT
GCCCAAGAAGACCGAGAGCCACCACAAGGCCAAGGGGAAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_178187
Insert Size 393 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178187.4, NP_835494.1
RefSeq Size 558 bp
RefSeq ORF 393 bp
Locus ID 319166
UniProt ID C0HKE9
Cytogenetics 13 A3.1
Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. provided by RefSeq, Aug 2015
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Documents
Resources
"NM_178187" in other vectors (4)
SKU Description Size Price
MG218068 Hist1h2ae (tGFP-tagged) - Mouse histone cluster 1 H2ae (Hist1h2ae), (10ug) 10 ug
$350.00
MR218068 Hist1h2ae (Myc-DDK-tagged) - Mouse histone cluster 1, H2ae (Hist1h2ae) 10 ug
$289.00
MR218068L3 Lenti ORF clone of Hist1h2ae (Myc-DDK-tagged) - Mouse histone cluster 1, H2ae (Hist1h2ae) 10 ug
$450.00
MR218068L4 Lenti ORF clone of Hist1h2ae (mGFP-tagged) - Mouse histone cluster 1, H2ae (Hist1h2ae) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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