Prrt1 (NM_030890) Mouse Untagged Clone

SKU MC214247

Prrt1 (untagged) - Mouse proline-rich transmembrane protein 1 (Prrt1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Prrt1
• Synonyms: Bat-4; Bat4; D17H6S54E; DSPD1; G5b; NG5; ORF31
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC214247 representing NM_030890; Red=Cloning site Blue=ORF; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTCGTCAGAAAAGTCAGGCCTTCCAGACTCGGTTCCCCACACTTCACCGCCGCCCTACAATGCCCCCC; AGCCACCGGCCGAGCCCCCCATCCCGCCCCCACAAACGGCCCCATCCTCACATCACCACCACCACCACCA; TTACCACCAGTCTGGCACCGCCACCCTCCCGCGCTTAGGAGCAGGTGGCCTGGCCTCTGCTGCGGCCAGT; GCTCAACGCGGTCCTTCGTCTTCCGCCACGCTACCGCGGCCCCCCCACCATGCCCCGCCCGGCCCCGCCG; CTGGGGCTCCCCCACCCGGCTGTGCTACCTTACCCCGCATGCCACCCGACCCTTATCTGCAGGAGACTCG; CTTCGAGGGTCCACTTCCCCCACCACCGCCTGCGGCCGCCGCCCCGCCCCCACCAGCGCCTGCCCCGACG; GCCCAAGCCCCGGGCTTCGTGGTTCCCACGCACGCGGGGGCGGTGGGCACGTTGCCGCTGGGGGGCTACG; TGGCGCCGGGCTACCCGCTGCAGCTGCAGCCGTGCACTGCTTATGTCCCTGTGTATCCGGTGGGCACGCC; CTACGCAGGCGGGACCCCCGGGGGTCCTGGAGTGACCTCCACTCTGCCCCCGCCGCCCCAGGGCCCAGGA; TTGGCTCTGTTGGAGCCCAGGCGTCCGCCACACGACTACATGCCCATCGCTGTGCTGACCACCATCTGCT; GCTTCTGGCCCACAGGCATCATCGCCATCTTCAAGGCCGTGCAGGTGCGCACGGCTTTGGCCCGCGGAGA; TTTGGTGTCTGCCGAGATCGCTTCACGCGAAGCCCGGAATTTCTCCTTCATCTCCCTGGCCGTGGGCATC; GCAGCCATGGTGCTTTGTACCATCCTCACTGTAGTCATCATCATCGCCGCCCAGCACCACGAAAACTACT; GGGATCCCTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI
• ACCN: NM_030890
• Insert Size: 921 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030890.1, NP_112152.1
• RefSeq Size: 921 bp
• RefSeq ORF: 921 bp
• Locus ID: 260297
• UniProt ID: O35449
• Cytogenetics: 17 B1
• Summary: Required to maintain a pool of extrasynaptic AMPA-regulated glutamate receptors (AMPAR) which is necessary for synapse development and function (PubMed:29490264). Regulates AMPAR function and synaptic transmission during development but is dispensable at mature hippocampal synapses (PubMed:29490264, PubMed:31216424). Plays a role in regulating basal phosphorylation levels of glutamate receptor GRIA1 and promotes GRIA1 and GRIA2 cell surface expression (PubMed:31216424).[UniProtKB/Swiss-Prot Function]