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Sdhaf1 (NM_001033140) Mouse Untagged Clone

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SKU
MC210719
Sdhaf1 (untagged) - Mouse succinate dehydrogenase complex assembly factor 1 (Sdhaf1), nuclear gene encoding mitochondrial protein, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $165.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sdhaf1
Synonyms 0610010E21Rik; AI430885; AW490662; Lyrm8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC210719 representing NM_001033140
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCCGGCCCAGCCGGTTGCAGAGGCAAGTTCTGAGCCTGTACCGCGAGCTGCTGCGCGCCGGGCGCG
GGACGCCGGGCGCCGAGGCGCGGGTGCGGGCCGAGTTCCGGCAGCACGCCAGCCTTCCGCGAACCGACGT
GCTGCGTATCGAGTATCTGTATCGCCGGGGTCGGCGCCAGCTACAGCTGCTGCGTTCGGGCCACGCCACG
GCCATGGGTACCTTCGTGCGCCCGCGGGGTCCGGCTGAAGAGCCCGGCGACGCGACAGCCCCGGGGACCA
GGCTGGATGATGGTGGTGCCCCAAAGAATTCTTGTGAAGATACAGGGGCGCGGGAGACGCGATCCGATGG
ACGGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001033140
Insert Size 357 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033140.3, NP_001028312.2
RefSeq Size 987 bp
RefSeq ORF 357 bp
Locus ID 68332
UniProt ID Q3U276
Cytogenetics 7 B1
Summary Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Promotes maturation of the iron-sulfur protein subunit Sdhb of the SDH catalytic dimer, protecting it from the deleterious effects of oxidants. May act together with SDHAF3. Contributes to iron-sulfur cluster incorporation into SDHB by binding to SDHB and recruiting the iron-sulfur transfer complex formed by HSC20, HSPA9 and ISCU through direct binding to HSC20.UniProtKB/Swiss-Prot Function
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"NM_001033140" in other vectors (4)
SKU Description Size Price
MG224575 Sdhaf1 (tGFP-tagged) - Mouse succinate dehydrogenase complex assembly factor 1 (Sdhaf1) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$350.00
MR224575 Sdhaf1 (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex assembly factor 1 (Sdhaf1), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR224575L3 Lenti ORF clone of Sdhaf1 (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex assembly factor 1 (Sdhaf1), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR224575L4 Lenti ORF clone of Sdhaf1 (mGFP-tagged) - Mouse succinate dehydrogenase complex assembly factor 1 (Sdhaf1), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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