Lsm5 (NM_025520) Mouse Untagged Clone

SKU
MC210352
Lsm5 (untagged) - Mouse LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm5), (10ug)
  $165.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Lsm5
Synonyms 2010208O10Rik; 2310034K10Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC210352 representing NM_025520
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTAACGCGACCACGAACCCGTCCCAACTCCTGCCACTAGAGCTTGTGGACAAATGTATAGGGT
CAAGAATTCACATTGTGATGAAGAGTGATAAAGAAATTGTCGGGACACTTCTAGGATTTGATGACTTTGT
CAATATGGTGTTGGAAGATGTCACAGAATTTGAAATTACACCAGAAGGAAGAAGAATTACAAAATTAGAT
CAAATTCTACTAAATGGAAATAATATAACAATGCTGGTTCCCGGAGGAGAAGGGCCTGAAGTATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_025520
Insert Size 276 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025520.3, NP_079796.1
RefSeq Size 516 bp
RefSeq ORF 276 bp
Locus ID 66373
UniProt ID P62322
Cytogenetics 6 B3
Summary Plays role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). The heptameric LSM2-8 complex binds specifically to the 3'-terminal U-tract of U6 snRNA.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025520" in other vectors (4)
SKU Description Size Price
MG200229 Lsm5 (tGFP-tagged) - Mouse LSM5 homolog, U6 small nuclear RNA associated (S 10 ug
$350.00
MR200229 Lsm5 (Myc-DDK-tagged) - Mouse LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm5) 10 ug
$289.00
MR200229L3 Lenti ORF clone of Lsm5 (Myc-DDK-tagged) - Mouse LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm5) 10 ug
$450.00
MR200229L4 Lenti ORF clone of Lsm5 (mGFP-tagged) - Mouse LSM5 homolog, U6 small nuclear RNA associated (S. cerevisiae) (Lsm5) 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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