Sgca (NM_001136080) Mouse Untagged Clone

SKU
MC209387
Sgca (untagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 1, (10ug)
  $503.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sgca
Synonyms 50DAG; 59kDa; adhalin; Asg
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC209387 representing NM_001136080
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCAGCAGTAACTTGGATACCTCTCCTGGCAGGTCTCCTGGCAGGACTGAGGGACACCAAGGCCC
AGCAGACAACTTTACACCTACTTGTGGGTCGTGTGTTTGTGCATCCTTTGGAACATGCCACCTTCCTGCG
CCTTCCAGAACACGTTGCGGTGCCACCCACTGTCCGACTCACCTACCACGCTCACCTCCAGGGACATCCA
GACCTGCCCAGGTGGCTGCACTACACACAGCGCAGTCCCTATAACCCTGGCTTCCTCTACGGCTCCCCCA
CTCCAGAAGATCGTGGGTACCAAGTCATCGAGGTCACAGCCTACAATCGAGACAGTTTTGACACCACTAG
ACAGAGGCTGCTGCTGCTGATTGGGGACCCCGAAGGTCCCCGGTTGCCATACCAAGCTGAGTTCCTGGTG
CGCAGCCATGATGTGGAGGAGGTGCTGCCCACCACACCTGCCAACCGCTTCCTCACCGCCTTGGGGGGAC
TGTGGGAGCCAGGAGAGCTTCAGCTGCTCAACATCACTTCCGCCTTGGACCGGGGAGGCCGAGTCCCTCT
TCCTATTGAGGGACGGAAGGAAGGGGTATACATTAAGGTAGGCTCTGCCACACCCTTCTCCACCTGCCTG
AAGATGGTGGCGTCGCCCGACAGCTATGCCCGTTGTGCCCAGGGACAGCCTCCACTACTGTCCTGCTACG
ACACTTTGGCACCCCACTTCCGCGTTGACTGGTGCAATGTGTCTCTGGTAGACAAGTCAGTACCCGAGCC
CCTGGATGAGGTACCTACTCCAGGCGATGGGATCTTGGAGCACGACCCGTTCTTCTGCCCACCCACTGAA
GCCACAGACCGAGACTTCCTGACAGATGCCTTGGTGACCCTCTTGGTGCCTTTGTTGGTGGCTCTGCTGC
TTACTCTGTTGCTGGCTTACATCATGTGCTTTCGGCGTGAAGGACGGCTGAAGAGAGACATGGCCACCTC
TGACATCCAGATGTTTCACCACTGTTCCATCCATGGGAATACAGAAGAGCTTCGGCAGATGGCAGCCAGC
CGAGAGGTGCCCCGGCCTCTTTCCACCTTGCCCATGTTTAATGTTCGTACAGGAGAGCGGTTACCTCCCC
GAGTAGACAGCGCACAGATGCCTCTTATCCTGGACCAGCACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001136080
Insert Size 1164 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001136080.1, NP_001129552.1
RefSeq Size 1465 bp
RefSeq ORF 1164 bp
Locus ID 20391
Cytogenetics 11 D
Summary This gene encodes a member of the sarcoglycan alpha/epsilon family of transmembrane proteins. The encoded protein is part of the dystrophin-glycoprotein complex which links the extracellular matrix to the cytoskeleton in muscle fibers. Disruption of this gene results in progressive muscular dystrophy and is associated with the development of embryonal rhabdomysarcoma. provided by RefSeq, Dec 2012
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Documents
Resources
"NM_001136080" in other vectors (4)
SKU Description Size Price
MG223392 Sgca (tGFP-tagged) - Mouse sarcoglycan alpha (dystrophin-associated glycoprotein) (Sgca) transcript variant 1, (10ug) 10 ug
$886.00
MR223392 Sgca (Myc-DDK-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 1 10 ug
$686.00
MR223392L3 Lenti ORF clone of Sgca (Myc-DDK-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 1 10 ug
$986.00
MR223392L4 Lenti ORF clone of Sgca (mGFP-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 1 10 ug
$986.00

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