Sftpb (NM_147779) Mouse Untagged Clone

SKU MC209384

Sftpb (untagged) - Mouse surfactant associated protein B (Sftpb), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sftpb
• Synonyms: AI562151; SF-B; Sftp-3; Sftp3; SP-B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209384 representing NM_147779; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCAAGTCGCACCTACTGCAGTGGCTACTGCTGCTTCCTACCCTCTGCTGCCCAGGTGCAGCTATCA; CGTCGGCCTCATCCCTGGAGTGTGCACAAGGCCCTCAATTCTGGTGCCAAAGCCTGGAGCATGCAGTGCA; GTGCAGAGCCCTGGGGCACTGCCTGCAGGAAGTCTGGGGGCATGCAGGAGCTAATGACCTGTGCCAAGAG; TGTGAGGATATTGTCCACCTCCTCACAAAGATGACCAAGGAAGATGCTTTCCAGGAAGCAATCCGGAAGT; TCCTGGAACAAGAATGTGATATCCTTCCCTTGAAGCTGCTTGTGCCCCGGTGTCGCCAAGTGCTTGATGT; CTACCTGCCCCTGGTTATTGACTACTTCCAGAGCCAGATTAACCCCAAAGCCATCTGCAATCATGTAGGC; CTGTGCCCACGTGGGCAGGCTAAGCCAGAACAGAATCCAGGGATGCCGGATGCCGTTCCAAACCCTCTGC; TGGACAAGCTGGTCCTCCCTGTGCTGCCAGGAGCCCTCTTGGCAAGGCCTGGGCCTCACACTCAGGACTT; CTCTGAGCAACAGCTCCCCATTCCCCTGCCCTTCTGCTGGCTTTGCAGAACTCTGATCAAGCGGGTTCAA; GCCGTGATCCCCAAGGGTGTGCTGGCTGTGGCTGTGTCCCAGGTGTGCCACGTGGTACCCCTGGTGGTGG; GTGGCATCTGCCAGTGCCTGGCTGAGCGCTACACAGTTCTCCTGCTAGACGCACTGCTGGGCCGTGTGGT; GCCCCAGCTAGTCTGTGGCCTTGTCCTCCGATGTTCCACTGAGGATGCCATGGGCCCTGCCCTCCCTGCT; GTGGAGCCTCTGATAGAAGAATGGCCACTACAGGACACTGAGTGCCATTTCTGCAAGTCTGTGATCAACC; AGGCCTGGAACACCAGTGAACAGGCTATGCCACAGGCAATGCACCAGGCCTGCCTTCGCTTCTGGCTAGA; CAGGCAAAAGTGTGAACAGTTTGTGGAACAGCACATGCCCCAGCTGCTGGCCCTGGTGCCTAGGAGCCAG; GATGCCCACATCACCTGCCAGGCCCTTGGCGTATGTGAGGCCCCGGCTAGCCCTCTGCAGTGCTTCCAAA; CCCCACACCTCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI
• ACCN: NM_147779
• Insert Size: 1134 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_147779.2, NP_680088.1
• RefSeq Size: 1564 bp
• RefSeq ORF: 1134 bp
• Locus ID: 20388
• UniProt ID: P50405
• Cytogenetics: 6 32.27 cM
• Summary: Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were based on transcript alignments.