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Oaz2 (NM_010952) Mouse Untagged Clone

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SKU
MC209068
Oaz2 (untagged) - Mouse ornithine decarboxylase antizyme 2, pseudogene (Oaz2-ps), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $165.00
4 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Oaz2
Synonyms AZ; AZ-; AZ-2; AZ2; Oaz; Oaz2-ps; Sez1; Sez15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC209068 representing NM_010952
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATAAACACCCAGGACAGTAGTATTTTGCCGTTGAGTAAGTGTCCCCAGCTCCAGTGCTGCAGGCACA
TTGTTCCAGGGCCTCTGTGGTGCTCCATGATAAACACCCAGGACAGTAGTATTTTGCCGTTGAGTAAGTG
TCCCCAGCTCCAGTGCTGCAGGCACATTGTTCCAGGGCCTCTGTGGTGCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010952
Insert Size 192 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010952.3, NP_035082.1
RefSeq Size 1861 bp
RefSeq ORF 571 bp
Locus ID 18247
Cytogenetics 9 C
Summary The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 2, the second member of the antizyme family. Like antizyme 1, antizyme 2 has broad tissue distribution, inhibits ODC activity and polyamine uptake, and stimulates ODC degradation in vivo; however, it fails to promote ODC degradation in vitro. Antizyme 2 is expressed at lower levels than antizyme 1, but is evolutionary more conserved, suggesting it likely has an important biological role. Studies also show different subcellular localization of antizymes 1 and 2, indicating specific function for each antizyme in discrete compartments of the cell. Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2014
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
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"NM_010952" in other vectors (4)
SKU Description Size Price
MG221992 Oaz2 (tGFP-tagged) - Mouse ornithine decarboxylase antizyme 2 pseudogene (Oaz2-ps), (10ug) 10 ug
$350.00
MR221992 Oaz2 (Myc-DDK-tagged) - Mouse ornithine decarboxylase antizyme 2, pseudogene (Oaz2-ps) 10 ug
$289.00
MR221992L3 Lenti ORF clone of Oaz2 (Myc-DDK-tagged) - Mouse ornithine decarboxylase antizyme 2, pseudogene (Oaz2-ps) 10 ug
$450.00
MR221992L4 Lenti ORF clone of Oaz2 (mGFP-tagged) - Mouse ornithine decarboxylase antizyme 2, pseudogene (Oaz2-ps) 10 ug
$450.00

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