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Nck2 (NM_010879) Mouse Untagged Clone

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SKU
MC209015
Nck2 (untagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Nck2
Synonyms 4833426I10Rik; Grb4; Nck-2; NCKbeta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC209015 representing NM_010879
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGAAGAAGTCATTGTCATAGCCAAGTGGGACTACACTGCCCAGCAGGACCAGGAGCTCGACATCA
GGAAGAACGAGCGGTTGTGGCTGCTAGATGACTCCAAGACGTGGTGGCGGGTGAGGAATGCTGCCAACAG
GACAGGCTACGTGCCTTCCAACTATGTGGAGCGTAAGAACAGCCTGAAGAAGGGCTCCCTGGTGAAGAAC
CTCAAGGACACACTAGGCCTCGGAAAGACGCGCAGGAAGCCAAGCGCTCGGGATGCTTCCCCAACGCCAA
GCACTGATGCGGAGTACCCAGCCAATGGCAGCGGTGCTGACCGCATCTATGACCTCAACATCCCAGCCTT
TGTCAAGTTCGCCTACGTAGCAGAACGAGAGGATGAGCTGTCCCTGGTGAAGGGCTCCCGTGTCACAGTT
ATGGAGAAATGCAGTGATGGCTGGTGGCGTGGCAGCTTCAACGGGCAGATCGGCTGGTTCCCCTCCAACT
ACGTTTTGGAGGAAGCGGACGAAGCTGCGGCTGAGGCCCCCAGCTTCCTGAGCCTACGTCGGGGCACTGC
ACTGAGCAATGGGCAGGGTGCACGGGTGCTGCACGTGGTGCAGACGCTGTACCCCTTCAGCTCGGTCACT
GAGGAGGAGCTTAGTTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAATGACCCTGAAT
GGTGGAAATGCAAAAATGCCCGAGGCCAAGTGGGCCTGGTCCCCAAAAACTACGTGGTTGTTCTCAGTGA
TGGGCCTGCTCTGCACCCCGCTCACACCCCCCAGATCAGCTACACCGGGCCTTCAGCCAGCGGGCGCTTT
GCTGGTCGGGAGTGGTACTATGGCAACGTGACACGGCACCAGGCCGAGTGTGCGCTCAATGAGCGGGGCG
TCGAGGGCGACTTCCTCATTAGGGACAGCGAGTCCTCGCCCAGTGACTTCTCCGTGTCTCTCAAAGCGTC
AGGGAGAAACAAGCACTTCAAGGTGCAGCTGGTGGACAGCGTCTACTGCATTGGGCAGCGGCGGTTCCAC
AGCATGGACGAGCTTGTGGAGCACTACAAGAAGGCCCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCT
ACCTTGTCCGAGCCCTACAGTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_010879
Insert Size 1143 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010879.3, NP_035009.3
RefSeq Size 2789 bp
RefSeq ORF 1143 bp
Locus ID 17974
UniProt ID O55033
Cytogenetics 1 C1.1
Summary Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling (By similarity).UniProtKB/Swiss-Prot Function
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"NM_010879" in other vectors (4)
SKU Description Size Price
MG227128 Nck2 (tGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2), (10ug) 10 ug
$657.00
MR227128 Nck2 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) 10 ug
$289.00 $457.00
MR227128L3 Lenti ORF clone of Nck2 (Myc-DDK-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) 10 ug
$757.00
MR227128L4 Lenti ORF clone of Nck2 (mGFP-tagged) - Mouse non-catalytic region of tyrosine kinase adaptor protein 2 (Nck2) 10 ug
$757.00

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