Fbxl2 (NM_178624) Mouse Untagged Clone

SKU MC207719

Fbxl2 (untagged) - Mouse F-box and leucine-rich repeat protein 2 (Fbxl2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Fbxl2
• Synonyms: 2810423A21Rik; Fbl3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207719 representing NM_178624; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGTTTTCTCAAACAGTGATGATGGCCTTATCAACAAGAAGCTACCCAAGGAGCTCCTCTTGAGAATAT; TCTCCTTCTTGGACATCGTAACTCTATGCCGATGTGCACAGATCTCCAAGGCCTGGAACATCTTAGCCCT; GGATGGCAGCAACTGGCAACGGGTGGATCTTTTTAACTTCCAGACAGATGTAGAGGGCCGAGTGGTGGAA; AACATCTCCAAGAGGTGCGGTGGCTTCCTTAGAAAGCTCAGCCTGCGTGGCTGCATCGGAGTCGGGGACT; CCTCTTTGAAGACCTTTGCACAGAACTGCCGGAACATTGAACATTTAAACCTCAATGGCTGCACGAAAAT; CACTGACAGCACGTGTTACAGCCTTAGCAGATTCTGTTCCAAGCTGAAACACCTGGATCTCACGTCCTGC; GTGTCTGTTACCAACAGCTCTTTAAAGGGCATCAGCGAGGGCTGCCGGAACCTGGAATATCTGAACCTCT; CCTGGTGTGACCAGATCACAAAGGAAGGCATTGAGGCGCTGGTGCGGGGGTGCCGGGGTTTGAAAGCCCT; GCTCCTGAGGGGTTGTACACAGTTAGAGGACGAAGCTCTGAAACACATTCAGAACCACTGCCACGAGCTG; GTGAGCCTCAACCTGCAGTCCTGCTCACGCATCACTGATGATGGCGTGGTGCAGATCTGCAGGGGCTGCC; ACCGGCTACAGGCTCTGTGCCTCTCGGGTTGTAGCAACCTTACGGATGCATCTCTCACAGCCTTGGGCCT; GAACTGCCCCAGACTACAAGTTTTGGAGGCTGCCCGGTGCTCCCATCTGACCGACGCAGGCTTTACACTG; CTAGCTCGGAATTGCCATGAGCTGGAGAAGATGGACCTTGAAGAATGTGTCCTGATTACCGACAGCACCC; TCGTCCAGCTCTCCATCCACTGTCCCAAGCTGCAAGCCCTGAGCTTGTCCCACTGTGAGCTCATCACAGA; TGAGGGGATCCTGCACCTGAGCAGCAGCACCTGTGGGCACGAGAGACTCCGGGTGCTGGAGCTGGACAAC; TGCCTTCTTGTCACGGACGCCTCGCTGGAGCACCTGGAGAACTGCCGAGGCCTGGAGCGACTGGAGCTTT; ACGACTGCCAGCAGGTCACCCGTGCAGGCATCAAGCGCATGCGGGCTCAGCTTCCTCATGTCAAAGTCCA; TGCCTACTTTGCTCCAGTCACCCCTCCACCAGCAGTGGCAGGAAGTGGACATCGACTGTGCAGATGCTGT; GTCATACTCTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_178624
• Insert Size: 1272 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_178624.6, NP_848739.1
• RefSeq Size: 3203 bp
• RefSeq ORF: 1272 bp
• Locus ID: 72179
• UniProt ID: Q8BH16
• Cytogenetics: 9 F3
• Summary: Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF(FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G(0). SCF(FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (By similarity). PCYT1A monoubiquitination by SCF(FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (PubMed:21343341).[UniProtKB/Swiss-Prot Function]