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Trmt10b (NM_027266) Mouse Untagged Clone

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SKU
MC207691
Trmt10b (untagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 3 (Rg9mtd3), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $330.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Trmt10b
Synonyms 2610042J10Rik; Rg9mtd3; Rnmtd3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC019428
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACTGTAAGTCGGAAGAGAGCGCCCAGAGAACAGACTCACAGGCCTTTCAGGAACCAGACGGACTCC
CAGAGGCCGGAGGTGAAGATGGACTCTCTGAAAGCTTCCAGCTTCTGCAGGTGGATGTGGAGTACGAGCG
CCCAGAAGAGACCAGCCCTGCAAACAGTGCCGTGTGGAGCTCGAAAAACATGCAAAGAAAACAGAGACAC
TGGGAAAGGATAGTCTCCTCAAAGAAGAGCAAAAGGAAGCAGGAAAGAGAACGAAGGAAAGCCAAGCGTG
CAGAAGACCCCGGTAACGGCACCTGCCCACAGCACAGCAAACGCTTTTTGAAAGCCTTAACCAAAGAGAA
ACTTTTGGAAGCCAAACACTCAGGACCAAGACTATGTGTTGACTTGAGCATGACCCAGCACATGTCAAAG
AAGGAATTGAGTAGGTTGGCGGGACAGATTCGAAGGTTGTATGGTTCGAACAAAAAAGCCAGCAGGCCCT
TTTGGATCTGCCTTACTGGATTCTCAACTGCCAGCCCCCTCTATGAGGAGTGTCTGAGGATGAACGACGG
GTTTTCTGCTTACTTGCTGGATGTGACAGAAGAAGACTGCTTTAGCCTTTTTCCTCTGGAAACCCTTGTG
TACCTGACTCCTGACTCCGAACACTCCCTTGAAGACATTGATCAGAGCACAGTTTACGTTATTGGTGGAC
TTGTAGATGAGAGCATTCAGAAGAAGGTGACATTTCAAAAAGCCCGAGAATACTCTGTCAAGACAGCCCG
CTTGCCAATCCAGGAATACATGATCAAGCGCCAGAATGAGAAAAACTACCATTCGGAAATATTGGCCATC
AACCAAGTGTTCGATATCCTGTCTACTTACTTTGAAACTCGTAACTGGCCTGAAGCACTGAAGAAAGGAG
TTTCTCCAGGAAAAGGCTACGTTCTTCAGAATTCAGCAGAAGGATGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_027266
Insert Size 957 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC019428, AAH19428
RefSeq Size 1329 bp
RefSeq ORF 957 bp
Locus ID 69934
UniProt ID Q9D075
Cytogenetics 4 B1
Summary S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs. Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs.UniProtKB/Swiss-Prot Function
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Documents
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"NM_027266" in other vectors (4)
SKU Description Size Price
MG204579 Trmt10b (tGFP-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 3 (Rg9mtd3) 10 ug
$500.00
MR204579 Trmt10b (Myc-DDK-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 3 (Rg9mtd3) 10 ug
$289.00 $300.00
MR204579L3 Lenti ORF clone of Trmt10b (Myc-DDK-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 3 (Rg9mtd3) 10 ug
$600.00
MR204579L4 Lenti ORF clone of Trmt10b (mGFP-tagged) - Mouse RNA (guanine-9-) methyltransferase domain containing 3 (Rg9mtd3) 10 ug
$600.00

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