Rprm (NM_023396) Mouse Untagged Clone

SKU
MC207643
Rprm (untagged) - Mouse reprimo, TP53 dependent G2 arrest mediator candidate (Rprm), (10ug)
$165.00
2 Weeks*
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Rprm
Synonyms 2410012A13Rik; Reprimo
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207643 representing NM_023396
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTCAGTGCTGGGCAACCAGACCGACGTGGCTGGCCTGTTCCTGGTCAACAGTAGCGAGGCGCTGG
AGCGCGCGGTGCGCTGCTGCACCCAGGCGTCGGTGGTGACCGACGATGGCTTCGCCGAGGGAGGCCCCGA
CGAGCGCAGCCTGTACATCATGCGTGTGGTGCAGATCGCAGTCATGTGTGTGCTCTCGCTCACTGTGGTT
TTTGGCATCTTCTTCCTTGGCTGCAACCTGCTCATCAAGTCCGAAGGCATGATCAACTTCCTAGTGAAGG
ACCGGAGGCCGTCTAAAGAGGTGGAGGCAGTGGTCGTGGGACCCTACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI
ACCN NM_023396
Insert Size 330 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023396.5, NP_075885.1
RefSeq Size 1460 bp
RefSeq ORF 330 bp
Locus ID 67874
UniProt ID Q9JJ72
Cytogenetics 2 C1.1
Summary May be involved in the regulation of p53-dependent G2 arrest of the cell cycle. Seems to induce cell cycle arrest by inhibiting CDK1 activity and nuclear translocation of the CDC2 cyclin B1 complex.[UniProtKB/Swiss-Prot Function]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.