Sdhaf2 (NM_025333) Mouse Untagged Clone

SKU
MC207558
Sdhaf2 (untagged) - Mouse succinate dehydrogenase complex assembly factor 2 (Sdhaf2), nuclear gene encoding mitochondrial protein, (10ug)
  $165.00
2 Weeks*
Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Sdhaf2
Synonyms 0610038F07Rik; AA407634; AW049997
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC207558 representing NM_025333
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGGTCACCTTGATCCCGACGCTGGCGCGGGCACTGTCAAAGCACAGTCTGCTGTCTCCCTTGC
CTAGTGTGACATCATTCAGACGCTTCTACAGAGGTGACAGCCCAACAGACTCTCAAAAAGACATGATTGA
AATCCCTTTGCCTCCGTGGCAGGAGAGAACTGATGAATCCATAGAAACCAAAAGAGCCCGCTTACTCTAT
GAGAGCAGAAAGAGGGGGATGCTGGAGAACTGCATTCTCCTTAGCCTGTTTGCTAAAGAGTATCTGCACA
ACATGACAGAGAAGCAGCTGAACCTCTATGATCGCCTGATTAACGAGCCTAGCAATGACTGGGATATTTA
CTACTGGGCCACAGAAGCAAAACCAGCCCCAGAAATATTTGAAAATGAAGTCATGGAATTGCTGAGAGAA
TTCGCCAAAAACAAAAACAAGGAGCAGAGACTGCGTGCCCCAGATCTGGAGTATCTCTTTGAAAAGCCTC
ATTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_025333
Insert Size 495 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025333.4, NP_079609.2
RefSeq Size 3116 bp
RefSeq ORF 495 bp
Locus ID 66072
UniProt ID Q8C6I2
Cytogenetics 19 A
Summary Plays an essential role in the assembly of succinate dehydrogenase (SDH), an enzyme complex (also referred to as respiratory complex II) that is a component of both the tricarboxylic acid (TCA) cycle and the mitochondrial electron transport chain, and which couples the oxidation of succinate to fumarate with the reduction of ubiquinone (coenzyme Q) to ubiquinol. Required for flavinylation (covalent attachment of FAD) of the flavoprotein subunit SDHA of the SDH catalytic dimer.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_025333" in other vectors (4)
SKU Description Size Price
MG201318 Sdhaf2 (tGFP-tagged) - Mouse RIKEN cDNA 0610038F07 gene (0610038F07Rik) 10 ug
$350.00
MR201318 Sdhaf2 (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex assembly factor 2 (Sdhaf2), nuclear gene encoding mitochondrial protein 10 ug
$289.00
MR201318L3 Lenti ORF clone of Sdhaf2 (Myc-DDK-tagged) - Mouse succinate dehydrogenase complex assembly factor 2 (Sdhaf2), nuclear gene encoding mitochondrial protein 10 ug
$450.00
MR201318L4 Lenti ORF clone of Sdhaf2 (mGFP-tagged) - Mouse succinate dehydrogenase complex assembly factor 2 (Sdhaf2), nuclear gene encoding mitochondrial protein 10 ug
$450.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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